FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n02_3022_d3_reseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n02_3022_d3_reseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17696736
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG523190.29564208902703865No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC283090.1599673521716095No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA271770.15357069235818402No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT210420.11890328250362102No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC207860.11745668805818203No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT204750.11569930183735577No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA188440.10648291300723478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT114450.046.0776671
AACCGTG117250.044.975022
ACCGTGT149450.035.3477443
CCGTGTT167150.031.604674
ACAACCG52450.031.1835162
CGTGTTC184900.028.6977845
TTCACTA233300.022.8449219
TGTTCAC240500.022.4150497
GTTCACT242800.022.1648
GTGTTCA253500.021.413886
AACTCAC178500.017.5885315
ACTCACC180150.017.349176
ATTAGGC56950.016.2578755
CACAACC109450.015.9320461
AGACTCA127800.015.7399834
GAACTCA226200.014.6067654
TATTAGG66450.014.14576154
AGAACTC230200.014.0875393
ACAGACT132900.013.8274822
CACTAGC221450.013.13734210-11
Produced by FastQC (version 0.11.2)