FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n02_3021_d5_reseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n02_3021_d5_reseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18292936
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG482890.26397621464372917No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC265740.14526919024917595No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA221610.12114512399759121No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC184940.10109913466050502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT108500.045.0077481
AACCGTG112550.042.760392
ACCGTGT143050.033.676213
CCGTGTT160800.030.01734
CGTGTTC176600.027.597855
ACAACCG53400.025.6997452
TTCACTA220350.022.203869
TGTTCAC229100.021.6430347
GTTCACT228150.021.5065548
GTGTTCA244700.020.724056
AACTCAC176150.018.303275
ACTCACC179100.017.4507166
GAACTCA219250.014.9410244
AGACTCA134850.014.533574
AGAACTC229650.013.7527663
CACTAGC199900.013.70720410-11
CACAACC109850.013.5631531
ACAGACT133050.013.4231962
TCACTAG201600.012.30938910-11
CTAGCAA229350.012.0296112-13
Produced by FastQC (version 0.11.2)