FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n01_4054_control.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n01_4054_control.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19648990
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG2869441.4603498704004634No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC1603100.8158689072568106No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC1570620.7993387955309662No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT1054900.5368723786820595No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC1024630.5214670067011078No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC960220.4886866958556139No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG866710.441096463482347No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA782240.39810697649090354No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG781310.39763366972042835No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC733520.37331180890213694No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT727590.3702938420753433No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA683510.3478601190188402No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC674080.34306089015262364No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA669080.3405162300963052No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC638110.32475460570746895No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC632520.32190967576450497No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA613470.3122145209499318No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT589150.299837294435999No Hit
GAAGAATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGC580770.29557244418160933No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA573380.2918114366183707No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA556730.2833377186308304No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC540880.275271146252301No Hit
CAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGG535650.272609431833392No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA531090.2702887018620296No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT523190.26626813897304646No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA522600.26596786908640085No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG500240.25458814931454493No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT499810.2543693085497015No Hit
CTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAG472940.24069430540704637No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA452730.23040878945940732No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC444020.22597599164130064No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG433420.22058131232190561No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC428770.21821477846952947No Hit
CAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGC426920.21727325424869165No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT423500.21553270677016986No Hit
GTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCA420790.2141535010196453No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT414330.2108658002268819No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA407380.2073287227485993No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG407300.2072880081876982No Hit
CTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCT406180.20671800433508286No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC403090.2051454044202781No Hit
CAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCC400010.20357789382558594No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA397210.20215288419404764No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA396940.20201547255100644No Hit
GTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAG390250.19861071739565242No Hit
CGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACC376320.1915212944787493No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC370960.18879341889837595No Hit
CTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGA368950.18777046555573595No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC362220.18434535311993136No Hit
CTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGCGGTGACGG342730.17442626822040216No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA341750.17392751484936375No Hit
CTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGG340360.17322009935370725No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT340110.17309286635089133No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC338390.17221750329151778No Hit
CTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCT335770.17088410142200694No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT335370.17068052861750144No Hit
CCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTG332700.16932168014742743No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG332310.1691231966630346No Hit
CGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGC328410.1671383618191062No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA323830.16480745320751855No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA320980.16335699697541706No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC319340.16252234847694463No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA314850.1602372437463707No Hit
CCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTC313300.15944839912891196No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC312350.15896491371821148No Hit
CGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGA308610.1570615079960853No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC299730.1525421917360638No Hit
AACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGG295890.15058789281281124No Hit
CTGTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGG295040.15015530060323712No Hit
ATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGC293270.1492544909433004No Hit
GTTATGAGCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTC290550.14787019587266317No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT287800.14647063284168804No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA279400.1421956039470731No Hit
CTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCAAGA278570.14177319037772423No Hit
CCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGCG276920.14093345255913917No Hit
CCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGC276090.14051103898979034No Hit
CTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTCGCTA274390.13964585457064205No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG272580.13872468763025478No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG270380.13760503720547468No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC267700.136241099415288No Hit
CAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTAT265800.13527412859388702No Hit
CTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTATCAGAAGGTGG257810.13120776182389018No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT257200.13089731329701934No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA255310.12993543179573097No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC255040.1297980201526898No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC253840.12918730173917337No Hit
GACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTG253280.1289022998128657No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG250410.12744166494053893No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA243320.12383333698067943No Hit
GTGGACTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGT236820.12052527890746548No Hit
CCCACTCAGACTTTATTCAGAGATCGGGAGGTTGAGGTGCGCGAAGCGCA236390.1203064381426221No Hit
GTCGCTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGG235090.11964482652797931No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG232660.11840812174060855No Hit
AGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCAC232300.11822490621655361No Hit
GGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTG230460.11728847131582845No Hit
CTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTA229310.11670319950287521No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA227550.11580747916305112No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC227150.11560390635854566No Hit
GGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTCTCTCCAGAG224670.11434175497061173No Hit
CACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAG224390.1141992540074579No Hit
CCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCC222910.11344603463078763No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA222850.11341549871011182No Hit
GTGCAGTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGA221380.11266736865355421No Hit
CCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGC221310.11263174341276574No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT220870.11240781332780972No Hit
CGGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCG219140.11152736094832355No Hit
CTCAGGAGCTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAG218960.11143575318629609No Hit
CCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTAT217960.11092682117503241No Hit
CGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC215670.10976136686923857No Hit
CAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGC214960.10940002514124136No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC214620.1092269882574117No Hit
GTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCAT211840.10781215726609866No Hit
AAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACAC211570.10767474562305747No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC211270.10752206601967837No Hit
GTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTC210040.10689607964582404No Hit
CAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGCGG209870.10680956120390922No Hit
CCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGAT209090.10641259423512354No Hit
GTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAA207250.10547615933439836No Hit
GGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCT204790.10422418658668971No Hit
GTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCAT204480.10406641766319796No Hit
CACGGCTCCGCCCAGGTCAAGGCCCACGGCAAGAAGGTGGCCGACGCGCT201720.1026617653121102No Hit
GGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCT200270.10192381389577784No Hit
CCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCAC199040.10129782752192351No Hit
AGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGC197780.10065657318773127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCG237650.066.431072
ACAGACT251750.065.753512
CACAGAC257800.064.673151
CACAACC263700.060.105861
CAACCGT547050.053.262641
AACCGTG579900.050.2339742
AGACTCA338750.049.143384
CAGACTC350350.047.583333
ACCGTGT664450.043.77783
CCGTGTT706000.041.247964
CGTGTTC740950.039.1818055
GTGTTCA805950.036.0976036
TGTTCAC806000.036.0545437
CTCAGAA479900.034.5912067
TCAGAAA486200.034.2106558
GTTCACT867800.033.4977658
TTCACTA880350.033.025579
CAGAAAG522800.031.7617059
CCGGATT22600.029.1264671
CGTCGCA8300.028.8908461
Produced by FastQC (version 0.11.2)