FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n01_4053_control.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n01_4053_control.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22262962
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG2755721.2378047449391505No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC1443280.6482875010072784No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC1434750.6444560252135363No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT996240.44748762541120984No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC916050.4114681595378009No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC872230.39178524402997233No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG804980.3615781224439048No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG728510.32722959325897427No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA714830.32108485834005374No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT680270.3055613174922546No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC662900.2977591211807306No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC650640.2922522169332185No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA649540.2917581227511416No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA630420.283169867513586No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC610270.2741189604509948No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC607890.2730499203115919No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA574970.2582630289716166No Hit
GAAGAATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGC558070.2506719456287982No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT551360.24765797111812884No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA530360.2382252640057509No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA515220.23142473135425554No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC514320.2310204724780108No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA506530.22752138731584773No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA502130.22554501058753995No Hit
CAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGG498890.2240896786330588No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT484560.21765297897018374No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT472680.21231676180375278No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG465480.20908269079379463No Hit
CTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAG449750.2020171439900944No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT416620.18713592557899528No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA411560.18486309234144135No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC411470.18482266645381687No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG411460.1848181746885253No Hit
CAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGC407080.18285078149080072No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA400670.17997155993887964No Hit
GTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCA395670.17772567729307537No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA389980.1751698628421501No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC388210.1743748203855354No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC388130.17433888626320254No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG386320.1735258767454214No Hit
CTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCT384220.1725826060341836No Hit
CGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACC382380.1717561212205276No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT377000.16933955149364222No Hit
CAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCC373510.16777192540687083No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA365550.16419648023475042No Hit
GTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAG365050.16397189197017No Hit
CTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGA360960.16213475996590213No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC354790.15936334078097963No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC353750.15889619719065234No Hit
CTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGCGGTGACGG334480.15024056547372266No Hit
CTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGG328430.1475230474722995No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC317320.1425326962333224No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT316200.14202961852066226No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA315470.14170171965437484No Hit
CGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGC313130.14065064657613843No Hit
CTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCT311170.13977026057898315No Hit
CCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTG310810.13960855702848526No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA309970.13923124874399012No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG308740.1386787616131223No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA307710.1382161097880866No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT307430.13809034035992157No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC305530.13723690495451593No Hit
CGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGA291110.1307597794040164No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC287570.12916969449078697No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA284150.12763351076105686No Hit
CCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTC283700.12743138132293447No Hit
ATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGC281660.12651506120344633No Hit
AACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGG280450.1259715576031617No Hit
CTGTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGG276590.12423773620060082No Hit
GTTATGAGCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTC269910.1212372369858063No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG267130.11998852623473912No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC266940.11990318269419856No Hit
CTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCAAGA266740.11981334738836638No Hit
CCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGCG264110.11863201311667333No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA263760.11847480133146704No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT259710.11665563638836557No Hit
CCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGC258730.11621544338978793No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC254110.11414024782506478No Hit
CTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTCGCTA253740.11397405250927527No Hit
GACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTG253700.11395608544810883No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG253620.11392015132577597No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT250520.11252770408537731No Hit
CAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTAT247870.11133738628310105No Hit
CTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTATCAGAAGGTGG245020.11005723317499261No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG241940.10867376946517718No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA241240.10835934589476458No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA239400.10753286108110861No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC238320.1070477504296149No Hit
GTGGACTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGT227070.10199451447655528No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC226610.10178789327314128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCG226250.066.185162
ACAGACT252800.058.564842
CACAACC269500.056.126431
CACAGAC274500.054.7101251
CAACCGT519300.052.7958561
AACCGTG551000.049.619332
AGACTCA327400.045.53554
ACCGTGT629450.043.441833
CAGACTC348900.042.635213
CCGTGTT666250.041.056454
CGTGTTC696900.039.2035565
TGTTCAC769950.035.5512967
GTGTTCA772250.035.5427936
GTTCACT822550.033.2264568
TTCACTA828000.033.1156049
CTCAGAA475450.030.9905517
TCAGAAA486550.030.409128
CAGAAAG532050.027.888099
CCGGATT28000.021.4944861
CGCCCTT37550.019.6591571
Produced by FastQC (version 0.11.2)