FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n01_3025_d5_reseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n01_3025_d5_reseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15068708
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG658610.43707131361228846No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC438700.29113312169829025No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA311870.20696532177808474No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC283470.18811831777482182No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT265430.17614648847134073No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC237920.1578901124104336No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT232290.15415389295485718No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA208080.13808748566897705No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC202030.13407254291476084No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC191080.12680582834308024No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC185710.12324215188189988No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG184140.12220025764650826No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG179270.11896839463608956No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG177900.1180592257810026No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA170290.11300902506040995No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC160770.10669129695790773No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC160310.106386028583207No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA158780.10537067942387628No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT154990.10285553346710281No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA152400.10113673979215737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT126050.051.996031
AACCGTG133650.048.7408562
ACCGTGT170150.038.3125043
ACAACCG52000.036.6058542
CCGTGTT186100.035.0036134
CGTGTTC202550.032.2536285
TGTTCAC251550.026.3258677
TTCACTA255250.025.5944069
GTGTTCA259400.025.4385976
GTTCACT261950.024.9218258
ACAGACT117000.022.1744142
AGACTCA124600.021.5007114
AACTCAC222550.021.4988985
CACAACC93700.021.3255181
ACTCACC226650.021.2136756
GAACTCA255250.019.315494
AGAACTC257650.019.0990853
CACAGAC159900.017.9362491
CAGACTC164200.017.3458463
TCACCAT296350.016.4622218
Produced by FastQC (version 0.11.2)