FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n01_3022_d3_reseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n01_3022_d3_reseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17696736
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG547160.3091869596743716No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC336460.1901254559032807No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA282090.1594022762163599No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT240370.13582730736334656No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC211580.11955877061171055No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT200970.1135633147265123No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA178820.10104688231773362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT109550.049.014681
AACCGTG120050.044.3580062
ACCGTGT154900.034.4078333
CCGTGTT173700.030.629674
CGTGTTC186600.028.6129345
ACAACCG48600.027.8522662
TGTTCAC242550.022.5552377
TTCACTA239850.022.3780429
GTTCACT250200.021.6401828
GTGTTCA259050.021.2637446
AACTCAC192350.019.6453425
ACTCACC191150.019.3752636
AGACTCA135500.017.3430884
GAACTCA231350.016.9430754
AGAACTC234900.016.4469173
ACAGACT139400.015.9141532
CACAACC101550.014.8626951
ATTAGGC61550.014.2030165
TCACCAT280550.013.8042458
CACAGAC183100.013.4046221
Produced by FastQC (version 0.11.2)