FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n01_3021_d5_reseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n01_3021_d5_reseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18292936
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG496660.2715037105033331No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC313240.17123549768063476No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA232810.12726770596037726No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT211680.11571679909665676No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC189190.10342243585174081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT111100.044.651
AACCGTG115250.042.6579972
ACCGTGT151550.032.656463
CCGTGTT170200.029.2713664
ACAACCG52100.029.1389922
CGTGTTC188500.026.304975
TTCACTA227850.021.8651439
TGTTCAC237800.021.4246677
GTTCACT237600.021.1261468
GTGTTCA258150.019.8996186
AACTCAC189500.018.7999485
ACTCACC190750.018.3564386
GAACTCA234300.015.6866844
AGACTCA138050.015.5247674
CACAACC108500.014.8644031
AGAACTC242250.014.7450573
ATTAGGC53700.014.1787315
ACAGACT137900.013.8720252
CACTAGC211100.013.34739310-11
CTTTATT242350.012.805131
Produced by FastQC (version 0.11.2)