FastQCFastQC Report
Wed 27 Sep 2017
H2W33BGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W33BGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363053206
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTGTCGGTCTCGTAGGACGCACTATTTACCATTCGATCGATGAATTCGAGCTCGTATCTCGTATGCCGTC3727810.10267944032423722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG148411150.068.8266146
TGTCGGT148438000.068.8152547
GTCGGTC148451300.068.7769248
TCGGTCT148533700.068.747689
CGGTCTC148618450.068.7204510
CCTCGAG2239900.068.70988
CTCGTAG135652000.068.7096714
ACGAGGT107446050.068.7080310
GGTCTCG146981900.068.69044511
AGGTCTC106233350.068.642613
GTCTCGT139864250.068.615312
CCGGGTG971100.068.612012
CACGAGG107721050.068.603749
CCACGAG116968050.068.5971458
GAGGTCT106636300.068.5905812
TTCGTGT3916800.068.569813
TCGTAGG34942750.068.56335415
TCCACGA117579550.068.467917
TCGTAGC33191250.068.4413715
CGTAGGG7448900.068.38499516
Produced by FastQC (version 0.11.2)