FastQCFastQC Report
Fri 3 Apr 2015
H2V3NBCXX l02n02 library 3 mfb.34200000004576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2V3NBCXX l02n02 library 3 mfb.34200000004576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28264950
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT1578130.5583346158404667No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG1569510.5552849023260257No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGG1386950.49069607411299154No Hit
CTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCG1202860.4255659394408976No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC1182130.4182317676132453No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC1017820.36009969945108694No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG960340.339763558753863No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT955560.3380724183131405No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT926980.32796095517593343No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC856500.3030254785520583No Hit
CCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC811960.2872674460772087No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG809720.28647494511753957No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT792820.280495808412893No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT777510.275079205871583No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA768700.2719622712935986No Hit
CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC728630.2577857027873745No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA664960.235259570598922No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA634600.22451835223483504No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT611920.21649428001818505No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT605250.21413446689274174No Hit
GTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG594430.2103064042214828No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC592140.2094962135082496No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG582190.20597595254900503No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT579640.20507377511723884No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA572040.2023849325755043No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC565730.20015248567572205No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA562230.19891420292623904No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG561930.19880806440485477Illumina Single End PCR Primer 1 (100% over 50bp)
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT558460.1975803955075102No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC553140.195698205728296No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG546620.19339146186354478No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG539300.19080168194176886No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC536630.18985704910144896No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG536460.1897969039393312No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA534000.1889265680639803No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG530950.18784749309657367No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC521170.18438737729944685No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT520530.1841609484538271No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC519910.18394159550963296No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG504210.17838701289052342No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG485660.17182411431826342No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC484600.1714490915427057No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC480450.16998084199689015No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA471440.16679314840464957No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG470370.16641458767837905No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGTGGGTC465730.16477297854763587No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA453220.16034700220591225No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC443900.15704963214157464No Hit
GTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCG439070.15534080194728808No Hit
CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGG438960.15530188448944718No Hit
CTTCCGTACGCCACATTTCCCGCGCCGCGCCGCGCGGCGGGGATTCGGCG430090.15216372220718594No Hit
CTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCA426490.15089005995057483No Hit
CTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGT426200.15078745937990337No Hit
CATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCC420300.1487000684593463No Hit
CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGG413830.14641101434815912No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA409060.1447234118581494No Hit
CTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT399120.14120668884961765No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC393040.13905561481623No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA389710.13787747722886473No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCGTCTC388110.13731140511481532No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGGGAGAGC387110.1369576100435345No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG386660.1367984022614581No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT379840.13438551987532263No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG373330.1320823139612842No Hit
CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG372430.13176389839713143No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT371290.13136057201587123No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG370170.13096432153603668No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCG363530.12861512226273175No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG360280.12746528828106896No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC357900.1266232560114205No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC352250.12462431385868364No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC349820.12376459183547114No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT346620.12263244760737238No Hit
CGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT343070.12137647510432532No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA341640.12087054815239369No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC338870.1198905358049457No Hit
CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG338360.11971010031859244No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG335490.11869470846401639No Hit
GTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGC334540.11835860314629958No Hit
CTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGC325020.11499047406770577No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC323250.11436425679153864No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT319780.11313658789419406No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT318070.11253159832230378No Hit
CTCCCGTCCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT317370.11228394177240718No Hit
CGGGTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGA317000.11215303759603325No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA315660.11167895220051689No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT313070.11076262296589945No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA312920.11070955370520734No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT312880.1106954019023561No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA309320.10943589144859624No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA307790.1088945849895365No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATG302910.1071680650416859No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTA302880.10715745118954749No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG296940.10505590846613916No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC295650.10459951282418684No Hit
CCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG295240.10445445684496169No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA293260.10375394260382557No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT293090.10369379744170784No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC289760.10251565985434256No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG289360.10237414182583023No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT288970.1022361617480307No Hit
CTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT288740.10215478888163608No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT287650.10176915225393995No Hit
CTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGG285470.10099787899854767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACCC31700.0112.50872145
CATCTCC26900.0104.01964145
CCTGGCC126400.097.998285
TCCGCTT54950.091.274512
TGGCCCG138600.089.5212867
TGCTCCC22100.086.594826145
CCCTCGT13450.083.525191
ACTTATG80450.083.14976
CTTATGC82350.081.759437
CCTCAAC35600.081.43631
CCAGACG86200.081.050336
GTAGGGG69350.081.00942145
GCCAGTA31350.078.6066743
AGACGTG48000.078.520954
TCCACTC99550.077.320452
TTATGCT89200.075.806238
CTCGATC94200.074.786541
CCAGTAG33300.074.222154
GCTTTTC64250.073.4397665
TCGATCA95800.073.3866962
Produced by FastQC (version 0.11.2)