##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2V3NBCXX l02n02 library 3 mfa.3420000000455d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22401474 Sequences flagged as poor quality 0 Sequence length 151 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.734916193461196 38.0 38.0 38.0 32.0 38.0 2 36.63152674685603 38.0 38.0 38.0 32.0 38.0 3 36.6790148719678 38.0 38.0 38.0 32.0 38.0 4 36.664230710889825 38.0 38.0 38.0 32.0 38.0 5 36.50765913885845 38.0 38.0 38.0 32.0 38.0 6 38.8049043558473 40.0 38.0 40.0 38.0 40.0 7 38.848047990056365 40.0 40.0 40.0 38.0 40.0 8 38.868837514888526 40.0 40.0 40.0 38.0 40.0 9 38.88305546322532 40.0 40.0 40.0 38.0 40.0 10-14 38.851146652224756 40.0 40.0 40.0 38.0 40.0 15-19 38.80032303231475 40.0 38.8 40.0 38.0 40.0 20-24 38.78739519551258 40.0 38.0 40.0 38.0 40.0 25-29 38.728910758283135 40.0 38.0 40.0 38.0 40.0 30-34 38.67026711724416 40.0 38.0 40.0 38.0 40.0 35-39 38.609484215190484 40.0 38.0 40.0 38.0 40.0 40-44 38.46331072678521 40.0 38.0 40.0 38.0 40.0 45-49 38.344483599605994 40.0 38.0 40.0 38.0 40.0 50-54 38.28063598850683 40.0 38.0 40.0 38.0 40.0 55-59 38.22520067206291 40.0 38.0 40.0 38.0 40.0 60-64 38.16617909160799 40.0 38.0 40.0 38.0 40.0 65-69 38.02635281946179 40.0 38.0 40.0 38.0 40.0 70-74 37.77789066023066 40.0 38.0 40.0 32.0 40.0 75-79 37.69790523605723 40.0 38.0 40.0 32.0 40.0 80-84 37.572302063694565 40.0 38.0 40.0 32.0 40.0 85-89 37.43926067543591 40.0 38.0 40.0 32.0 40.0 90-94 37.3033395748869 39.6 38.0 40.0 32.0 40.0 95-99 37.11807565877139 38.0 38.0 40.0 32.0 40.0 100-104 35.648286501147204 38.0 35.8 38.8 29.0 39.2 105-109 37.27340441972702 39.2 38.0 40.0 32.0 40.0 110-114 37.21687848754952 39.6 38.0 40.0 32.0 40.0 115-119 36.94927326657165 38.0 38.0 40.0 32.0 40.0 120-124 36.592093181011215 38.0 38.0 40.0 30.0 40.0 125-129 36.28072350060536 38.0 38.0 40.0 27.0 40.0 130-134 35.83679070404028 38.0 38.0 40.0 25.0 40.0 135-139 35.39733780018226 38.0 38.0 40.0 22.0 40.0 140-144 34.88181540196864 38.0 35.6 40.0 22.0 40.0 145-149 34.25663873725453 38.0 32.0 40.0 13.0 40.0 150-151 29.255915391996083 30.0 22.5 39.0 13.0 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.038591013640619565 2201 2 0.15748846471069555 2201 3 0.17618832284126995 2201 4 0.19629111354379347 2201 5 0.2305111611319859 2201 6 0.1620158417401285 2201 7 0.15745526710776403 2201 8 0.1423191254032119 2201 9 0.09571964028579316 2201 10-14 0.1136176188257636 2201 15-19 0.1391373758996295 2201 20-24 0.12457541684035789 2201 25-29 0.11481296421929699 2201 30-34 0.1311600071516139 2201 35-39 0.0907278918874681 2201 40-44 0.14831677560500367 2201 45-49 0.12011444738842414 2201 50-54 0.11659629568217866 2201 55-59 0.11476249052435605 2201 60-64 0.10834670561424531 2201 65-69 0.08285791041083002 2201 70-74 0.12128324724233153 2201 75-79 0.07197234562576682 2201 80-84 0.09797211989865673 2201 85-89 0.1060425567157921 2201 90-94 0.056400180223384666 2201 95-99 -0.005079950521633236 2201 100-104 0.20091518839046074 2201 105-109 0.21390586968008307 2201 110-114 0.21853008649281236 2201 115-119 0.20876223697235474 2201 120-124 0.25035872029182826 2201 125-129 0.16476544352881461 2201 130-134 0.1974690419344043 2201 135-139 0.2289003323299923 2201 140-144 0.19569970329345665 2201 145-149 0.245474854447977 2201 150-151 0.020540761641374417 1101 1 0.07050023420296725 1101 2 0.09097017146173414 1101 3 0.1143650399577183 1101 4 0.14051283791486924 1101 5 0.13673751186780692 1101 6 0.18834022967793373 1101 7 0.1507331790920361 1101 8 0.15674329340148319 1101 9 0.15030255712566287 1101 10-14 0.14332162457279907 1101 15-19 0.12135315599741148 1101 20-24 0.11483713562883224 1101 25-29 0.151244725618362 1101 30-34 0.15337768566728016 1101 35-39 0.19088173536014352 1101 40-44 0.20394997426645034 1101 45-49 0.17493501734569605 1101 50-54 0.1553999151362575 1101 55-59 0.1505817856160192 1101 60-64 0.1565761580751115 1101 65-69 0.13826308927872333 1101 70-74 0.15932865334477242 1101 75-79 0.1423851545283199 1101 80-84 0.1286971775988377 1101 85-89 0.10247021723676397 1101 90-94 0.13310281510104005 1101 95-99 0.033122910185845456 1101 100-104 0.2786531881697485 1101 105-109 0.2468206122802883 1101 110-114 0.2961225881195304 1101 115-119 0.3137051462497382 1101 120-124 0.3570918087516617 1101 125-129 0.311654375975138 1101 130-134 0.2978388958639471 1101 135-139 0.31391934807666644 1101 140-144 0.3314474052069727 1101 145-149 0.34513702464677465 1101 150-151 0.23329414132418336 2202 1 -0.09397701165566019 2202 2 -0.04103507194366074 2202 3 0.02432023231853009 2202 4 0.08661962149386682 2202 5 0.023284611779942566 2202 6 0.09948585540833932 2202 7 0.0911135431510317 2202 8 0.09161975004084866 2202 9 0.09623174682337776 2202 10-14 0.06103632118047386 2202 15-19 0.06836919477913028 2202 20-24 0.05557985952847844 2202 25-29 0.07218558045416756 2202 30-34 0.07031956796932093 2202 35-39 0.049191615744454964 2202 40-44 0.07754745047926548 2202 45-49 0.04260715838027096 2202 50-54 0.03167113219407014 2202 55-59 -0.002245471022497725 2202 60-64 0.024453228794229176 2202 65-69 0.04309027006678434 2202 70-74 0.04317330247074125 2202 75-79 0.04127654391493962 2202 80-84 0.04048967844217799 2202 85-89 0.05323697370257463 2202 90-94 0.017942498947085994 2202 95-99 0.004245868563565125 2202 100-104 0.15795136015428568 2202 105-109 0.1664161434613476 2202 110-114 0.19223183682873923 2202 115-119 0.23589002179043916 2202 120-124 0.19873038434961643 2202 125-129 0.24949191584162378 2202 130-134 0.21082164289656902 2202 135-139 0.2621262067168857 2202 140-144 0.26992994454273145 2202 145-149 0.28114461670408986 2202 150-151 0.5641628785912793 1102 1 -0.09495698496699845 1102 2 0.00895928480368724 1102 3 0.0061709564271836825 1102 4 0.017945172526012243 1102 5 -0.01934628153474449 1102 6 0.05398494173756774 1102 7 0.037635656684273044 1102 8 0.028374554267557528 1102 9 0.058547895995893384 1102 10-14 0.036157752151723344 1102 15-19 0.017527555408463513 1102 20-24 0.007833241700552662 1102 25-29 0.033636322050668355 1102 30-34 0.0533035063610896 1102 35-39 0.059398659270733845 1102 40-44 0.02562475639000894 1102 45-49 0.009565191021927433 1102 50-54 -0.006212484652451167 1102 55-59 -0.011546732412824667 1102 60-64 -0.0129548418611094 1102 65-69 0.0061589105147135115 1102 70-74 0.05755255694766248 1102 75-79 -0.002259317980197295 1102 80-84 -0.00964445280786208 1102 85-89 0.02357216994276712 1102 90-94 0.02543157061197121 1102 95-99 2.4380531236545266E-5 1102 100-104 0.1848857451000825 1102 105-109 0.10729131723628882 1102 110-114 0.15519029146324215 1102 115-119 0.17178644596108938 1102 120-124 0.27531507612133765 1102 125-129 0.18821389861897586 1102 130-134 0.1870114989671663 1102 135-139 0.19685918266143432 1102 140-144 0.22574607045581985 1102 145-149 0.3610133266670559 1102 150-151 0.7016497703700288 2203 1 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0.2300513284585719 2203 130-134 0.25578839950436105 2203 135-139 0.26859727867137195 2203 140-144 0.36349553323695005 2203 145-149 0.4045001793284797 2203 150-151 0.7589440439207742 1103 1 -0.1495308075556352 1103 2 -0.06965891880415143 1103 3 -0.13245012280785318 1103 4 -0.08645189885442761 1103 5 -0.30762307536927125 1103 6 -0.09668243270092347 1103 7 -0.06583278538045079 1103 8 -0.014392143948860792 1103 9 -0.05988464803306215 1103 10-14 -0.02962886007502874 1103 15-19 -0.016102964515326335 1103 20-24 -0.047332221911688066 1103 25-29 -0.06962449698565365 1103 30-34 -0.06846049469923088 1103 35-39 -0.06544997317989498 1103 40-44 -0.0706004018322588 1103 45-49 -0.08646521120205364 1103 50-54 -0.11710489064311957 1103 55-59 -0.11639795900156713 1103 60-64 -0.08547205839414573 1103 65-69 -0.10166259689910362 1103 70-74 -0.036014411264680746 1103 75-79 -0.0634350030338382 1103 80-84 -0.07403938615596672 1103 85-89 -0.12012507895685332 1103 90-94 -0.08568408484794787 1103 95-99 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125-129 -2.6700663011469743 1213 130-134 -3.2106882618363954 1213 135-139 -3.7977849403584045 1213 140-144 -3.930702371088202 1213 145-149 -3.9790285988864866 1213 150-151 -4.085706428225951 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 621.0 4 696.0 5 491.0 6 879.0 7 628.0 8 459.0 9 253.0 10 283.0 11 278.0 12 237.0 13 274.0 14 408.0 15 2097.0 16 6012.0 17 10908.0 18 18063.0 19 26362.0 20 37602.0 21 52972.0 22 69152.0 23 84361.0 24 102100.0 25 138273.0 26 196714.0 27 172155.0 28 177945.0 29 212544.0 30 258209.0 31 321134.0 32 400912.0 33 506677.0 34 659000.0 35 892186.0 36 1303873.0 37 2187941.0 38 4957303.0 39 9601460.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.162300946509973 10.854684981068461 5.681180474420212 53.301833598001345 2 24.111746083109125 13.361779388333922 38.543743778709015 23.98273074984794 3 23.534882674357117 16.511590591661545 19.370883575124324 40.58264315885701 4 29.299557337416932 21.936786086599355 16.889544189244393 31.874112386739323 5 31.26911676446405 21.673936902416603 21.161919795832475 25.895026537286874 6 26.16324052621946 26.8138679451639 23.275604540807898 23.747286987808742 7 20.54359118627268 18.5422549235238 34.05856262786377 26.85559126233975 8 22.666876477323708 18.37394508764059 27.99366550377994 30.965512931255763 9 24.484767834101113 18.36818390513151 28.46902040367187 28.678027857095508 10-14 28.361651458143022 22.08894984343862 22.47761381741195 27.071784881006412 15-19 28.566181638119186 22.48607141698315 21.932204065391023 27.015542879506643 20-24 28.57776095271487 22.200746207116833 21.619208812578627 27.60228402758967 25-29 29.122845268359665 22.02696957833614 21.62696607428819 27.223219079016005 30-34 28.46138954466369 21.86412139320039 21.906390484314194 27.768098577821725 35-39 27.366166671668047 21.722276641988433 22.433926425428723 28.477630260914793 40-44 29.025190365857718 20.564181300694383 22.084338402277286 28.326289931170614 45-49 28.657549795221925 20.59703289405569 21.78089311840284 28.96452419231954 50-54 28.228636283032454 22.301332004796617 21.182795968835226 28.287235743335703 55-59 28.469211766753272 21.15345458926735 21.908875803094073 28.468457840885304 60-64 28.171152340187106 21.35068600547404 21.58521660034801 28.89294505399085 65-69 28.09146619387091 21.868621050956946 21.947457664380373 28.092455090791766 70-74 28.86913516730585 21.870918605166693 21.405570864174138 27.854375363353324 75-79 29.201935992931205 22.262124961533367 21.42074847734479 27.115190568190638 80-84 29.749321874129368 21.237055939991386 22.171442689732995 26.842179496146244 85-89 28.81303737721785 22.463392485503604 21.613968485226053 27.109601652052497 90-94 29.106814159741802 22.85743342909699 21.28726422780527 26.748488183355946 95-99 29.27957934889133 22.68935292262241 20.837126760485887 27.193940968000373 100-104 29.321780269323256 23.544898474181633 20.77132968846687 26.361991568028238 105-109 29.701959702588752 23.4476470255607 20.87013118253966 25.98026208931089 110-114 30.763970650662444 23.491627837988684 20.844513286395426 24.899888224953447 115-119 29.718769090090944 24.280673473783747 20.023372274592383 25.97718516153293 120-124 30.35928419417944 24.60824241786049 19.804322221993218 25.22815116596685 125-129 30.466446595092904 25.218364389793603 19.26484260388923 25.05034641122426 130-134 30.870435178789375 25.238297532285703 19.572611486632894 24.31865580229203 135-139 30.2985759149065 26.074291074205746 20.050068605167507 23.577064405720243 140-144 31.26544010129454 26.958011104339707 19.88782517458444 21.888723619781313 145-149 31.0581952393021 28.048199191372973 19.597329893834505 21.296275675490424 150-151 30.930719659017008 29.34776707627212 18.05974176078169 21.66177150392918 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 662.0 1 496.5 2 344.0 3 383.5 4 451.5 5 505.0 6 504.5 7 495.5 8 498.5 9 503.0 10 518.5 11 512.0 12 511.5 13 568.5 14 634.0 15 664.0 16 668.5 17 662.5 18 673.0 19 696.0 20 776.0 21 849.5 22 966.0 23 1252.0 24 1648.5 25 1843.0 26 1923.0 27 2156.5 28 2815.5 29 3743.0 30 5510.5 31 6239.0 32 5639.5 33 6579.5 34 7870.5 35 10087.0 36 16060.0 37 24897.5 38 30852.0 39 36218.5 40 51491.0 41 72009.5 42 126254.5 43 210281.0 44 383439.5 45 605086.0 46 912244.0 47 1119365.0 48 1104266.0 49 982874.5 50 820486.5 51 889755.5 52 986182.0 53 982427.5 54 1000007.0 55 1025871.5 56 1009798.0 57 867085.0 58 835677.0 59 825159.5 60 783642.0 61 733774.5 62 575683.5 63 528012.5 64 441569.0 65 347070.0 66 338504.5 67 330564.5 68 323173.5 69 298419.0 70 268572.5 71 239993.5 72 189573.0 73 171120.5 74 197483.0 75 156517.0 76 121851.0 77 144694.0 78 140646.0 79 132774.0 80 142728.5 81 110186.0 82 165537.0 83 165604.5 84 82110.5 85 82375.5 86 71570.5 87 54411.0 88 45131.0 89 23502.5 90 2457.0 91 1388.0 92 991.5 93 776.5 94 263.0 95 129.0 96 89.0 97 63.5 98 50.0 99 53.0 100 69.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010463597172221792 2 0.011543883228398274 3 0.012454537589803243 4 0.013052712513471213 5 0.011664411011525402 6 7.76734602374826E-4 7 8.526224658252399E-4 8 0.0014463333975255378 9 0.0016025731163940374 10-14 0.005580882757982801 15-19 0.008906556773898002 20-24 0.016387314513321757 25-29 0.020178136492268323 30-34 0.0263964773032346 35-39 0.03414953855268631 40-44 0.04304895293943604 45-49 0.04884589290865413 50-54 0.05593649775010341 55-59 0.053589330773501774 60-64 0.06073707471213725 65-69 0.05754889164882632 70-74 0.05866310404395711 75-79 0.056204337268163686 80-84 0.049496742937540625 85-89 0.05039668371822319 90-94 0.045387191932102326 95-99 0.04317930150489204 100-104 0.03165684543793859 105-109 0.02621077523737947 110-114 0.02659021455463154 115-119 0.023951102503344198 120-124 0.021961054884156284 125-129 0.022061941102625656 130-134 0.020437048026393262 135-139 0.021269136129167216 140-144 0.022342279798195423 145-149 0.018313080648175207 150-151 0.014159782521453723 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 2.2401474E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 3.1217919713124624 #Duplication Level Percentage of deduplicated Percentage of total 1 72.48885118850203 2.262951136499295 2 9.233261636864341 0.5764864409398094 3 4.5075468474253 0.42214870675821314 4 2.27731490195572 0.28437213508302384 5 2.171316715555033 0.3389199544898124 6 1.0281315701272031 0.1925767728645587 7 0.9584026112637277 0.20943535039896033 8 0.7215864358320412 0.18021141935907536 9 0.4626610113725139 0.1299898287667816 >10 3.5986179993460112 2.3278004431138695 >50 0.6942061090928892 1.496052524781464 >100 1.0164305555423527 7.552332073827292 >500 0.29730524062393365 6.599197443583734 >1k 0.4234650651259992 29.22249478550772 >5k 0.07347187577271867 15.866177703205004 >10k+ 0.04743023559812311 32.33885328082152 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 158505 0.7075650468357574 Illumina Single End PCR Primer 1 (100% over 50bp) CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG 115970 0.5176891484908538 No Hit CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT 104549 0.46670589622807857 No Hit CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGG 79058 0.3529142769801666 No Hit CTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCG 77279 0.34497283526967915 No Hit CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC 75176 0.33558506016166617 No Hit CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG 74521 0.33266114542284136 No Hit CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC 74449 0.332339738001169 No Hit CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT 71145 0.31759070853998267 No Hit CCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC 66512 0.29690903375376104 No Hit CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA 63775 0.28469108773824436 No Hit CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC 63589 0.2838607852322575 No Hit CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT 63180 0.28203501251747987 No Hit CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC 60142 0.2684734049196941 No Hit CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT 59461 0.2654334263897099 No Hit GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA 57370 0.256099219185309 No Hit CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC 57258 0.2555992520849298 No Hit GTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG 54842 0.2448142474910356 No Hit CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT 52202 0.23302930869638308 No Hit CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG 48943 0.21848115887374195 No Hit CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG 48646 0.21715535325934357 No Hit GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC 48062 0.21454838195022344 No Hit CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG 45945 0.20509811095466307 No Hit GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA 45788 0.20439726421573864 No Hit CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT 45502 0.20312056251298463 No Hit CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG 45209 0.20181261286645694 No Hit CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC 43778 0.19542464036071913 No Hit GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT 43163 0.19267928530060122 No Hit CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC 41977 0.1873849908269429 No Hit CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC 40946 0.18278261510827368 No Hit CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG 40786 0.18206837639344625 No Hit CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA 40727 0.18180500086735363 No Hit GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG 39270 0.17530096457045638 No Hit ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG 38985 0.17402872685967002 No Hit CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT 38973 0.17397515895605797 No Hit CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA 37444 0.1671497152374884 No Hit GTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCG 36619 0.16346692186415948 No Hit CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA 36388 0.16243573971962738 No Hit CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC 36155 0.16139562959115994 No Hit CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA 36046 0.16090905446668374 No Hit CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT 35742 0.15955200090851163 No Hit CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGG 35511 0.15852081876397955 No Hit GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT 35494 0.15844493090052914 No Hit CATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCC 35271 0.1574494606917384 No Hit CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG 34461 0.15383362719792457 No Hit CTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCA 33252 0.1484366609090098 No Hit CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA 32585 0.14545917826657298 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG 32423 0.1447360115678102 No Hit CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGG 32355 0.14443246011400857 No Hit AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC 31335 0.13987918830698373 No Hit CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC 31260 0.13954438890940837 No Hit GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT 31167 0.13912923765641494 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 30072 0.13424116645181472 No Hit CTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT 29160 0.13017000577729843 No Hit CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA 29047 0.12966557468495152 No Hit CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC 28811 0.12861207258058108 No Hit CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT 28759 0.12837994499826216 No Hit CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG 28581 0.12758535442801666 No Hit CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC 28260 0.12615241300639413 No Hit CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT 28117 0.12551406215501712 No Hit CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC 28101 0.12544263828353439 No Hit CTTCCGTACGCCACATTTCCCGCGCCGCGCCGCGCGGCGGGGATTCGGCG 27939 0.1247194715847716 No Hit CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA 27931 0.12468375964903025 No Hit AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG 27904 0.1245632318659031 No Hit CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG 27837 0.12426414440406912 No Hit CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT 27757 0.1239070250466554 No Hit GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC 27580 0.12311689846837756 No Hit CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC 27559 0.12302315463705647 No Hit CTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT 26720 0.11927786537618017 No Hit AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA 26276 0.11729585294253406 No Hit CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCGTCTC 25848 0.1153852643803707 No Hit CGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT 25706 0.11475137752096135 No Hit AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG 25418 0.11346574783427196 No Hit GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTA 24970 0.11146587943275518 No Hit CGGGTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGA 24897 0.11114000801911517 No Hit AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC 24804 0.11072485676612172 No Hit GTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGC 24642 0.11000169006735896 No Hit CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG 24548 0.10958207482239785 No Hit CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA 24407 0.10895265195495618 No Hit CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCG 24390 0.10887676409150576 No Hit GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC 24299 0.10847054082244766 No Hit CTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGT 24027 0.10725633500724104 No Hit GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT 24012 0.10718937512772597 No Hit CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGTGGGTC 23886 0.10662691213979937 No Hit GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC 23822 0.1063412166538684 No Hit CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG 23615 0.10541717031656041 No Hit GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC 23599 0.10534574644507767 No Hit CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG 23146 0.10332355808372252 No Hit CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC 22911 0.10227451997131974 No Hit CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT 22869 0.10208703230867755 No Hit CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG 22859 0.10204239238900083 No Hit CTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGGGAGAGC 22790 0.1017343769432315 No Hit GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC 22523 0.10054249108786323 No Hit GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC 22522 0.10053802709589557 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC 22428 0.10011841185093445 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001209741823238953 0.0 0.0 2 0.00123206178307731 0.0 0.0 3 0.0013659815421074523 0.0 0.0 4 0.001428477429654852 0.0 0.0 5 0.0014999013011375948 0.0 0.0 6 0.0016829249718121227 0.0 0.0 7 0.0017186369075534941 0.0 0.0 8 0.001848092674615965 0.0 0.0 9 0.001932908522001722 0.0 0.0 10-11 0.0022074440280135136 0.0 0.0 12-13 0.0025199234657505127 0.0 0.0 14-15 0.0030756904657256038 0.0 0.0 16-17 0.003923848939583172 0.0 0.0 18-19 0.004698351545974162 0.0 0.0 20-21 0.005829973509778865 0.0 0.0 22-23 0.008497208710462535 0.0 0.0 24-25 0.012729073095815033 0.0 0.0 26-27 0.019556748810368462 0.0 0.0 28-29 0.031957718496559644 0.0 0.0 30-31 0.05009491786120859 0.0 0.0 32-33 0.0742116344665534 0.0 0.0 34-35 0.10243968767412359 0.0 0.0 36-37 0.1355825960381 0.0 0.0 38-39 0.17422291051026373 0.0 0.0 40-41 0.22143855355232428 0.0 0.0 42-43 0.2796066008870666 0.0 0.0 44-45 0.341374857743736 0.0 0.0 46-47 0.4127898012425433 0.0 0.0 48-49 0.5047480357765743 0.0 0.0 50-51 0.5989427302864088 0.0 0.0 52-53 0.705056283349926 0.0 0.0 54-55 0.8297333470110047 0.0 0.0 56-57 0.9709963728279666 0.0 0.0 58-59 1.121763237544101 0.0 0.0 60-61 1.289689687383964 0.0 0.0 62-63 1.4714991522432854 0.0 0.0 64-65 1.684525312932533 0.0 0.0 66-67 1.9090328609626313 0.0 0.0 68-69 2.161252871128034 0.0 0.0 70-71 2.4555035976650466 0.0 0.0 72-73 2.7689338656911593 0.0 0.0 74-75 3.084803705327605 0.0 0.0 76-77 3.434856563456494 0.0 0.0 78-79 3.8141887449013403 0.0 0.0 80-81 4.223838127794627 0.0 0.0 82-83 4.65798366661051 0.0 0.0 84-85 5.135456711464611 0.0 0.0 86-87 5.645914192967838 0.0 0.0 88-89 6.185358606313138 0.0 0.0 90-91 6.770021026294966 0.0 0.0 92-93 7.432548858168886 0.0 0.0 94-95 8.116847578869141 0.0 0.0 96-97 8.838831319760477 0.0 0.0 98-99 9.621766406978399 0.0 0.0 100-101 10.456128913659878 0.0 4.463991967671413E-6 102-103 11.30243929484283 0.0 4.463991967671413E-6 104-105 12.18379647696397 0.0 4.463991967671413E-6 106-107 13.128453065186694 0.0 4.463991967671413E-6 108-109 14.07337526093149 0.0 4.463991967671413E-6 110-111 15.111298033334771 0.0 4.463991967671413E-6 112-113 16.18966903695712 0.0 4.463991967671413E-6 114-115 17.234359221183393 0.0 4.463991967671413E-6 116-117 18.335150624463374 0.0 4.463991967671413E-6 118-119 19.587932026258628 0.0 4.463991967671413E-6 120-121 20.921839786078362 0.0 4.463991967671413E-6 122-123 22.286957545740073 0.0 4.463991967671413E-6 124-125 23.737647799426057 0.0 4.463991967671413E-6 126-127 25.361480677566128 0.0 4.463991967671413E-6 128-129 27.07742356596713 0.0 4.463991967671413E-6 130-131 28.650404879607475 0.0 4.463991967671413E-6 132-133 30.26652844361938 0.0 4.463991967671413E-6 134-135 31.878272831510998 0.0 4.463991967671413E-6 136-137 33.53980858581002 0.0 4.463991967671413E-6 138-139 35.20439994261092 0.0 4.463991967671413E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATCTCC 1795 0.0 103.78588 145 AGAACCC 2205 0.0 100.267654 145 CTCCGAT 895 0.0 97.983826 1 TCCGCTT 4650 0.0 96.16767 2 CCTGGCC 10420 0.0 90.841225 5 CCCTCGT 965 0.0 90.125145 1 TGCTCCC 1860 0.0 89.63641 145 TGGCCCG 10940 0.0 86.71359 7 CGGTGGG 4020 0.0 82.04553 145 GTAGGGG 5995 0.0 79.19936 145 CCTCAAC 3025 0.0 78.58516 1 CCCCTGT 715 0.0 77.03703 1 GCTTTTC 5555 0.0 76.71863 5 CTTATGC 8510 0.0 75.622025 7 ACTTATG 8465 0.0 75.510185 6 TTTTCTC 5790 0.0 74.223434 7 CGCCCTT 1280 0.0 74.174286 1 CATTCCT 3480 0.0 72.27092 4 CCTCACC 14565 0.0 71.654594 1 TCCACTC 7545 0.0 71.37177 2 >>END_MODULE