FastQCFastQC Report
Fri 3 Apr 2015
H2V3NBCXX l02n02 library 3 mfa.3420000000455d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2V3NBCXX l02n02 library 3 mfa.3420000000455d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22401474
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1585050.7075650468357574Illumina Single End PCR Primer 1 (100% over 50bp)
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG1159700.5176891484908538No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT1045490.46670589622807857No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGG790580.3529142769801666No Hit
CTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCG772790.34497283526967915No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC751760.33558506016166617No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG745210.33266114542284136No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC744490.332339738001169No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT711450.31759070853998267No Hit
CCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC665120.29690903375376104No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA637750.28469108773824436No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC635890.2838607852322575No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT631800.28203501251747987No Hit
CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC601420.2684734049196941No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT594610.2654334263897099No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA573700.256099219185309No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC572580.2555992520849298No Hit
GTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG548420.2448142474910356No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT522020.23302930869638308No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG489430.21848115887374195No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG486460.21715535325934357No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC480620.21454838195022344No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG459450.20509811095466307No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA457880.20439726421573864No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT455020.20312056251298463No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG452090.20181261286645694No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC437780.19542464036071913No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT431630.19267928530060122No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC419770.1873849908269429No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC409460.18278261510827368No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG407860.18206837639344625No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA407270.18180500086735363No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG392700.17530096457045638No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG389850.17402872685967002No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT389730.17397515895605797No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA374440.1671497152374884No Hit
GTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCG366190.16346692186415948No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA363880.16243573971962738No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC361550.16139562959115994No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA360460.16090905446668374No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT357420.15955200090851163No Hit
CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGG355110.15852081876397955No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT354940.15844493090052914No Hit
CATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCC352710.1574494606917384No Hit
CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG344610.15383362719792457No Hit
CTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCA332520.1484366609090098No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA325850.14545917826657298No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG324230.1447360115678102No Hit
CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGG323550.14443246011400857No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC313350.13987918830698373No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC312600.13954438890940837No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT311670.13912923765641494No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA300720.13424116645181472No Hit
CTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT291600.13017000577729843No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA290470.12966557468495152No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC288110.12861207258058108No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT287590.12837994499826216No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG285810.12758535442801666No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC282600.12615241300639413No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT281170.12551406215501712No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC281010.12544263828353439No Hit
CTTCCGTACGCCACATTTCCCGCGCCGCGCCGCGCGGCGGGGATTCGGCG279390.1247194715847716No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA279310.12468375964903025No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG279040.1245632318659031No Hit
CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG278370.12426414440406912No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT277570.1239070250466554No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC275800.12311689846837756No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC275590.12302315463705647No Hit
CTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT267200.11927786537618017No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA262760.11729585294253406No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCGTCTC258480.1153852643803707No Hit
CGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT257060.11475137752096135No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG254180.11346574783427196No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTA249700.11146587943275518No Hit
CGGGTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGA248970.11114000801911517No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC248040.11072485676612172No Hit
GTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGC246420.11000169006735896No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG245480.10958207482239785No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA244070.10895265195495618No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCG243900.10887676409150576No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC242990.10847054082244766No Hit
CTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGT240270.10725633500724104No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT240120.10718937512772597No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGTGGGTC238860.10662691213979937No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC238220.1063412166538684No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG236150.10541717031656041No Hit
GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC235990.10534574644507767No Hit
CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG231460.10332355808372252No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC229110.10227451997131974No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT228690.10208703230867755No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG228590.10204239238900083No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGGGAGAGC227900.1017343769432315No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC225230.10054249108786323No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC225220.10053802709589557No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC224280.10011841185093445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCC17950.0103.78588145
AGAACCC22050.0100.267654145
CTCCGAT8950.097.9838261
TCCGCTT46500.096.167672
CCTGGCC104200.090.8412255
CCCTCGT9650.090.1251451
TGCTCCC18600.089.63641145
TGGCCCG109400.086.713597
CGGTGGG40200.082.04553145
GTAGGGG59950.079.19936145
CCTCAAC30250.078.585161
CCCCTGT7150.077.037031
GCTTTTC55550.076.718635
CTTATGC85100.075.6220257
ACTTATG84650.075.5101856
TTTTCTC57900.074.2234347
CGCCCTT12800.074.1742861
CATTCCT34800.072.270924
CCTCACC145650.071.6545941
TCCACTC75450.071.371772
Produced by FastQC (version 0.11.2)