FastQCFastQC Report
Fri 3 Apr 2015
H2V3NBCXX l02n02 library 3 l3b.342000000045b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2V3NBCXX l02n02 library 3 l3b.342000000045b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17530766
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC3136601.7891973459687955No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG2168591.2370195346854782No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1441420.8222230563114012No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1182320.6744257495650788No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1157220.6601080637320696No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC702780.40088379480965064No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG672180.38342876745944815No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC664850.3792475468556252No Hit
CTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTG633010.36108519159972813No Hit
CTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAAACTAACGAGGATTT613410.3499048472839122No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC609680.34777715930952474No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG598810.34157663162009005No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT545350.3110816720729716No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA507930.2897363412414494No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC487930.27832782663347394No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA485580.27698732616703686No Hit
CTCAGAATGAGGAGGTAGTGACGAAAAATAACGAGACCGTTCTCTTTGAG469130.267603822901977No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT450320.2568741149131761No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG448070.25559065701977884No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA429680.24510052783774536No Hit
CTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAATATG413490.2358653352625892No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA408700.23313299601397908No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT405070.23106235061263153No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC394930.22527823370638794No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA383740.21889516978322565No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC378450.21587761766941618No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA375850.21439451077037933No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT372710.21260337397692716No Hit
GTCGTGATTACCCGCTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAA370900.21157090340490542No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA370410.21129139479701No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC365720.20861609812143975No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG365340.20839933634388824No Hit
ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA356630.2034309282321149No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT356560.20339099843098696No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG353060.20139450837459127No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC339730.1937907333883756No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA338800.19326023745910476No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC331220.18893641042268206No Hit
CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG330510.1885314081540989No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT329550.1879837994529161No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA320280.18269595293211946No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG319500.1822510208624084No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT304400.1736375923333869No Hit
CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA298180.17008954429030654No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC296460.16910841203402063No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC296460.16910841203402063No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA294710.1681101670058228No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG293670.16751692424620807No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC292910.167083400691105No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA292560.16688375168546543No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT290350.16562311082128414No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT283740.16185259674334823No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA279580.15947962570488933No Hit
GTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTGCAATCT278260.15872666374076294No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA278090.15862969136659516No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA277990.1585726487935553No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG277750.15843574661825957No Hit
CTCAGGACCTGGTCCATCCATTGGATGAGAACTAGGCTCCTAGGCTAATA275440.1571180631810384No Hit
CTCAGATCAGAAAACCAACAAAATAGAACCGAGATCCTATTTCATTATTC274340.15649059487759975No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT273120.15579467548651327No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC271040.15460818996728382No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG268460.15313649158285497No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG267590.15264022119740803No Hit
CCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATT267390.15252613605132828No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT264820.15106014192420342No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC264700.1509916908365556No Hit
CTCAGTCGTGATTACCCGCTGAATTTAAGCATATAACTAAGCGGAGGAAA258690.14756343219685894No Hit
GCTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAAACTAACGAGGATT256910.14654807439674913No Hit
CTCACGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTGCCC254840.14536729313482366No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG252910.144266371475154No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG249690.14242960062326998No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG245200.13986838909377947No Hit
CTGAAGTTTCCCTCAGGATAGCTGGAGTACGTTAAATAAAGCAGTTATAT244890.13969155711735587No Hit
CTCTTTTCTGGTTAAGGAACGGACTCCCTGGAATCGGTTCAGCCGGAGAT242960.1385906354576862No Hit
CTTCCGTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGA242780.13848795882621445No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA240080.13694780935413775No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG238870.13625759422035524No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA237700.13559019611578865No Hit
CTGAATACTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGT236760.1350539959292138No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC232540.132646799346931No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG229140.13070735186357516No Hit
CTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATC226910.12943530248478588No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT226830.129389668426354No Hit
GGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTTCAA225000.12834578933972424No Hit
CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGC221060.12609831196195306No Hit
CTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAG220810.12595570552935337No Hit
GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA220390.12571612672258586No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC220240.12563056286302607No Hit
CTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGGAAG218490.1246323178348282No Hit
CTCAAAGGCTAAGCCATGCATGTCTAAGTTCAAATAAACCTATAATGGTG214570.12239624897166501No Hit
CTCAATGCGGGCTAATGCCTGAATACTCGTGCATGGAATAATGAAATAGG212860.12142082097268313No Hit
CTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTTCAAATAAACCTATAA212160.12102152296140398No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT210660.12016588436580582No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT208100.11870559449598496No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT207840.11855728380608126No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG207240.118215028367842No Hit
CTTTTACTCTACGAGAGGTTTCCGTCCTCTCTGAGCTAGCCTTAGGACAC206420.117747279268915No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC205320.11711981096547636No Hit
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGC205160.11702854284861255No Hit
CTCAGGTATAGTTCACCATCTTTCGGGTCCCAGCATATACGCTCTACCTC204550.1166805831530693No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA201900.11516895496751255No Hit
CTAGCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAA201060.11468979735397758No Hit
CGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAGAA198770.11338352243136438No Hit
GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT197620.1127275328414058No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG196500.11208865602335917No Hit
CTGCTTACCAAAAATGGCCCACTTGGAGCACACATTCAATGTCTATGCTT196270.11195745810536745No Hit
CATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTGCA195110.11129576425810486No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA194770.11110181950976929No Hit
CGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAGCCCAACGCTGA193530.1103944916040748No Hit
CTTTCATCGATTCTTTACTTATTCGGTAAAACGAGAATTGAAATATTATA191800.10940765509048492No Hit
CTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAAAGAGGGTGGTAAACCTC191420.1091908933129334No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA191110.10901406133650977No Hit
CTCTCATAGATCCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACC191090.1090026528219018No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA190790.10883152510278217No Hit
CTTCAACCTATTCTCAAACTTTCAATGAGTATGACGTCTTGCTTGCTTAA189030.10782757581728032No Hit
CTCGTTTAAAATTCGGAGCTTTGGTGCATGTATTAGCTCTAGAATTGCCA188970.10779335027345639No Hit
CTCACGATGAGCATTTATCTCCGTGTCCAGGTACGGGAATATTAACCCGT187010.10667531584187478No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT186720.10650989238005916No Hit
CTCGTTATTTTTCGTCACTACCTCCTCATTCTGAGAGTGGGTAATTTGCG186220.10622467951485975No Hit
CTCGTAGTTGGATCTGCGTCTCAGGACCTGGTCCATCCATTGGATGAGAA184550.1052720685450938No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC181630.1036064254123294No Hit
CTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGC180720.1030873379976665No Hit
CTCATCGTGAGAATTTAGCGCGGTAACGCAAATGAACTCGGAAACATTGA180620.10303029542462663No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCTCATAGATCCTC178460.10179817584696528No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA176330.10058316904121589No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTT175510.1001154199422889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCAG401650.0116.810753
CCAGACG407950.0115.561216
CAGACGT413750.0114.0466847
TGGCTCA291700.0111.3183063
ACCTGGC157900.0108.6078644
CCTGGCC161050.0106.483295
CACCTGG162600.0105.556783
CTTGTTA97450.0105.540551
CTAGCAT62800.097.871221
GTCCAAT28750.092.018221
GTTACGA111500.091.983584
CTCAACC532750.090.921551
TACGCAA67150.090.766989
TCCAATC29900.090.419352
TTACGAC114950.089.285665
AGTCGTG44900.089.27044
ATACGCA69750.087.5908668
TGGCCCG196650.087.1217357
CTGGCCC202350.084.631546
CGCCCTT14500.084.476811
Produced by FastQC (version 0.11.2)