##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2V3NBCXX l02n02 library 3 l3a.34200000004590.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15032517 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.76378107538478 38.0 38.0 38.0 32.0 38.0 2 36.540410032464955 38.0 38.0 38.0 32.0 38.0 3 36.5241948504033 38.0 38.0 38.0 32.0 38.0 4 36.56609462008259 38.0 38.0 38.0 32.0 38.0 5 36.414898449807175 38.0 38.0 38.0 32.0 38.0 6 38.64109097631488 40.0 38.0 40.0 38.0 40.0 7 38.66277510279882 40.0 38.0 40.0 38.0 40.0 8 38.67260858577443 40.0 38.0 40.0 38.0 40.0 9 38.693512603378394 40.0 38.0 40.0 38.0 40.0 10-14 38.708466865528905 40.0 38.0 40.0 38.0 40.0 15-19 38.70484743173748 40.0 38.0 40.0 38.0 40.0 20-24 38.715159078150386 40.0 38.0 40.0 38.0 40.0 25-29 38.655580858481656 40.0 38.0 40.0 38.0 40.0 30-34 38.59706936636094 40.0 38.0 40.0 38.0 40.0 35-39 38.498306531101875 40.0 38.0 40.0 38.0 40.0 40-44 38.32679899181221 40.0 38.0 40.0 38.0 40.0 45-49 38.20264903076445 40.0 38.0 40.0 38.0 40.0 50-54 38.12899525741431 40.0 38.0 40.0 38.0 40.0 55-59 38.07008332669772 40.0 38.0 40.0 38.0 40.0 60-64 38.04850910862099 40.0 38.0 40.0 38.0 40.0 65-69 37.963133479243695 40.0 38.0 40.0 38.0 40.0 70-74 37.755670377755095 40.0 38.0 40.0 32.0 40.0 75-79 37.69471383933908 40.0 38.0 40.0 32.0 40.0 80-84 37.56923172613075 40.0 38.0 40.0 32.0 40.0 85-89 37.46619290701617 40.0 38.0 40.0 32.0 40.0 90-94 37.36903182614062 40.0 38.0 40.0 32.0 40.0 95-99 37.209969827408145 40.0 38.0 40.0 32.0 40.0 100-104 35.969849373860676 38.4 35.8 38.8 30.0 39.2 105-109 37.43994768141623 39.6 38.0 40.0 32.0 40.0 110-114 37.423462178689036 40.0 38.0 40.0 32.0 40.0 115-119 37.20719671895266 40.0 38.0 40.0 32.0 40.0 120-124 36.93981576072723 39.2 38.0 40.0 32.0 40.0 125-129 36.74577554776755 38.0 38.0 40.0 32.0 40.0 130-134 36.42122325888605 38.0 38.0 40.0 28.0 40.0 135-139 36.11556357461628 38.0 38.0 40.0 27.0 40.0 140-144 35.75554398508247 38.0 38.0 40.0 24.0 40.0 145-149 35.35987539545108 38.0 38.0 40.0 22.0 40.0 150-151 30.454581524837124 32.5 25.5 39.0 17.5 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.10697144617508769 2201 2 0.18770371861713642 2201 3 0.25083700616738014 2201 4 0.24579228326913238 2201 5 0.29395342906247635 2201 6 0.26005700783250063 2201 7 0.2646419857080602 2201 8 0.222530692446675 2201 9 0.15988966558241202 2201 10-14 0.1913465799898617 2201 15-19 0.2041250505542891 2201 20-24 0.1978484423601472 2201 25-29 0.19859717833063684 2201 30-34 0.19595764713373853 2201 35-39 0.17347256536890399 2201 40-44 0.20596340220672715 2201 45-49 0.1877694932060976 2201 50-54 0.17322810523103982 2201 55-59 0.17420226505993242 2201 60-64 0.1801340456707834 2201 65-69 0.14822341314988563 2201 70-74 0.1973897910486997 2201 75-79 0.1487380860183336 2201 80-84 0.18461226041690537 2201 85-89 0.1823140729259265 2201 90-94 0.1550543964636617 2201 95-99 0.0887495150996429 2201 100-104 0.25058811938841075 2201 105-109 0.2885910777569478 2201 110-114 0.2851834368708239 2201 115-119 0.26966822166863125 2201 120-124 0.3278814402294046 2201 125-129 0.22413062898683478 2201 130-134 0.2996583995095605 2201 135-139 0.3338512676073009 2201 140-144 0.3245155859905964 2201 145-149 0.33568786122432925 2201 150-151 0.2909624686099477 1101 1 0.1306767283755974 1101 2 0.17979967189718593 1101 3 0.17173403642081553 1101 4 0.20632745589702495 1101 5 0.18169129968648434 1101 6 0.2431535386975625 1101 7 0.22791313678972358 1101 8 0.21469972465000353 1101 9 0.2125273479809806 1101 10-14 0.16192038810422105 1101 15-19 0.18665059240072424 1101 20-24 0.17041911616235694 1101 25-29 0.18982124548290358 1101 30-34 0.1932706391860819 1101 35-39 0.2355313000654604 1101 40-44 0.24917065227154467 1101 45-49 0.2153171354337644 1101 50-54 0.18417271051732342 1101 55-59 0.1804746926981764 1101 60-64 0.15271055498119068 1101 65-69 0.13418762252927507 1101 70-74 0.1775264075918983 1101 75-79 0.1832204890167759 1101 80-84 0.16372221207004145 1101 85-89 0.15800404765268183 1101 90-94 0.15336435964524497 1101 95-99 0.08643081611732129 1101 100-104 0.3264035418779301 1101 105-109 0.319517609993504 1101 110-114 0.3396297840288369 1101 115-119 0.3410361165503417 1101 120-124 0.3722309793345886 1101 125-129 0.33614980272426465 1101 130-134 0.3419037128332647 1101 135-139 0.3516088975081715 1101 140-144 0.41802170279091655 1101 145-149 0.4180156105336934 1101 150-151 0.381757610926428 2202 1 0.009955620447748004 2202 2 0.005588875582525077 2202 3 0.015848584004523047 2202 4 0.14264199049533488 2202 5 0.11054277925858003 2202 6 0.15163194708665628 2202 7 0.13317350483337975 2202 8 0.11860718588361152 2202 9 0.11922155546553626 2202 10-14 0.13945817722485288 2202 15-19 0.1354594252450596 2202 20-24 0.10251276754326 2202 25-29 0.13107411100680366 2202 30-34 0.1306040450265229 2202 35-39 0.12052015799552152 2202 40-44 0.1387946398671076 2202 45-49 0.09933019036583346 2202 50-54 0.08593255801577016 2202 55-59 0.07947854480654826 2202 60-64 0.08983249180213448 2202 65-69 0.11923902330210012 2202 70-74 0.1191702216963435 2202 75-79 0.11419367400831959 2202 80-84 0.10388716032564815 2202 85-89 0.14408859783605266 2202 90-94 0.07139058332056436 2202 95-99 0.06549703814537366 2202 100-104 0.2370632245843609 2202 105-109 0.23146397364162397 2202 110-114 0.26377137856423616 2202 115-119 0.3005670285839983 2202 120-124 0.2919795037443649 2202 125-129 0.3363338107029108 2202 130-134 0.33952321741336533 2202 135-139 0.3458630822236799 2202 140-144 0.31940115447280704 2202 145-149 0.3085904605341767 2202 150-151 0.6791981285447015 1102 1 0.03384983649372941 1102 2 -0.022500490093136705 1102 3 -0.016820677088723812 1102 4 0.06213361870099732 1102 5 -0.0017576155547445182 1102 6 0.09145459960795677 1102 7 0.10736494804918095 1102 8 0.056812839111188396 1102 9 0.07844499224447787 1102 10-14 0.08276650410228825 1102 15-19 0.0727307725090185 1102 20-24 0.07297236475923796 1102 25-29 0.0936244927227321 1102 30-34 0.10388852233711532 1102 35-39 0.09097826143315046 1102 40-44 0.07328976405710819 1102 45-49 0.056034020798684026 1102 50-54 0.009836671132596564 1102 55-59 0.01465907335129657 1102 60-64 0.036840535186200896 1102 65-69 0.03905825743220959 1102 70-74 0.10277399899335649 1102 75-79 0.016572884128883913 1102 80-84 0.040299619124105845 1102 85-89 0.08498674678705953 1102 90-94 0.07893515653677952 1102 95-99 0.03346282731258299 1102 100-104 0.23460402471594222 1102 105-109 0.18901603784696164 1102 110-114 0.18676090409691426 1102 115-119 0.22029360426100197 1102 120-124 0.3229374352711929 1102 125-129 0.2596444064062027 1102 130-134 0.30985901411727923 1102 135-139 0.2931695188843477 1102 140-144 0.3058982099932237 1102 145-149 0.4119542625266419 1102 150-151 0.7633550666167537 2203 1 -0.029933397323716804 2203 2 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130-134 0.21649875390642137 2203 135-139 0.20673480923855436 2203 140-144 0.30374405412712235 2203 145-149 0.3854157760049901 2203 150-151 0.7223110586058858 1103 1 -0.06417785547930066 1103 2 -0.12909298469033814 1103 3 -0.12335849381832986 1103 4 -0.054355767173127845 1103 5 -0.28270155446500667 1103 6 -0.06566509201938686 1103 7 -0.02331923781748202 1103 8 -0.010182622763274196 1103 9 -0.04373241002521411 1103 10-14 -0.03716522291745861 1103 15-19 -0.0342151032098954 1103 20-24 -0.05854846540141523 1103 25-29 -0.059108789505799564 1103 30-34 -0.06098082580079023 1103 35-39 -0.06706069257876379 1103 40-44 -0.056107122626478656 1103 45-49 -0.09175059762980453 1103 50-54 -0.10635356321017753 1103 55-59 -0.09858595331321851 1103 60-64 -0.10123474876619554 1103 65-69 -0.09147825754813965 1103 70-74 -0.023819624903829606 1103 75-79 -0.07684669487389328 1103 80-84 -0.07809951739115206 1103 85-89 -0.10972350662746067 1103 90-94 -0.11175962194143096 1103 95-99 -0.11548393607891683 1103 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130-134 -2.8550462677979667 1213 135-139 -3.3384253957919796 1213 140-144 -3.4807516523906585 1213 145-149 -3.649529637161571 1213 150-151 -4.1042932672432 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 435.0 4 473.0 5 350.0 6 551.0 7 417.0 8 305.0 9 169.0 10 200.0 11 184.0 12 167.0 13 197.0 14 234.0 15 708.0 16 2445.0 17 6975.0 18 15984.0 19 25840.0 20 37082.0 21 53958.0 22 70979.0 23 76142.0 24 79511.0 25 89587.0 26 110733.0 27 107747.0 28 117991.0 29 138308.0 30 165747.0 31 201538.0 32 246135.0 33 297719.0 34 373311.0 35 493024.0 36 701752.0 37 1144640.0 38 2729294.0 39 7741676.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.771788992656766 11.424089068287213 6.366229915486571 53.43789202356945 2 18.8853714491539 15.439484701399172 51.13962186624694 14.535521983199985 3 20.525130848601496 20.353269601676033 24.806093915598247 34.31550563412422 4 26.734348460443773 34.70880462096399 19.217923666539594 19.33892325205264 5 26.824408529554294 31.868010871511938 22.08722943302452 19.220351165909246 6 18.297373446339034 35.79779486945463 25.764245997212154 20.140585686994182 7 15.576856234057338 17.403106692650567 44.547122761956935 22.47291431133516 8 17.790353648124057 20.926191341786012 32.2091241435534 29.074330866536524 9 25.099025303498763 20.31453801152707 31.68683982438047 22.8995968605937 10-14 23.585740978303306 27.422669688623362 26.5178788874181 22.473710445655232 15-19 23.115426191092023 26.49038379081502 26.912570998052193 23.481619020040768 20-24 24.26194741165229 27.48656905539318 25.431465802095683 22.82001773085885 25-29 23.766503812364125 27.6522238300563 25.29975447964661 23.28151787793297 30-34 22.34625877108371 27.56265014166176 26.04058605126738 24.050505035987154 35-39 23.161891269751635 27.692274808540972 26.250469778699397 22.895364143007995 40-44 22.537172174790598 28.020100150692567 27.719352584331236 21.723375090185606 45-49 22.621041032862255 27.033769989635058 27.55846623373081 22.786722743771875 50-54 23.293516736140475 28.259793282662987 25.687139850531043 22.759550130665495 55-59 22.971449299718955 26.43451602651034 28.10260294555662 22.491431728214078 60-64 22.99349220277525 26.32520790240755 28.015720987301584 22.66557890751562 65-69 22.976413771021555 28.05622008343245 26.81101141351423 22.15635473203177 70-74 22.127339911744144 28.027401837198585 26.81078371676719 23.034474534290084 75-79 22.53715256691951 27.84204154299384 27.15452832573305 22.4662775643536 80-84 23.814130614830635 26.43404281329739 26.656453805639153 23.095372766232824 85-89 22.143514597294082 28.406138781206785 27.25847874005598 22.19186788144315 90-94 23.679395558389942 25.725863726892022 28.232211649867384 22.362529064850648 95-99 22.94671119444074 27.09282973940359 26.622760217798174 23.33769884835749 100-104 24.362017297077106 25.20300314710575 27.701551023736993 22.73342853208015 105-109 23.68026426614098 26.95288667135199 27.222887255575845 22.143961806931184 110-114 24.036221555238097 27.254769083340076 27.442843116502097 21.266166244919734 115-119 23.975043608490488 28.57247246916485 25.329084293761234 22.123399628583428 120-124 24.92723679083301 28.225560585510255 25.016388805517636 21.8308138181391 125-129 25.77154900974932 28.54866207722462 24.403671021337562 21.276117891688497 130-134 25.4407746341546 28.62691645614791 24.80118210056446 21.131126809133033 135-139 27.25798499379943 28.57546489000718 24.12475163437919 20.041798481814197 140-144 27.58983640378946 28.024181386508136 24.006841281801762 20.379140927900643 145-149 27.29140734446646 28.400513377282948 24.50653958020872 19.80153969804187 150-151 25.29717898522029 30.664603905062403 24.352093659117134 19.686123450600174 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 99.0 1 94.5 2 108.0 3 183.0 4 269.5 5 327.0 6 358.5 7 385.5 8 428.5 9 505.5 10 587.0 11 680.0 12 813.5 13 944.0 14 1408.0 15 2379.5 16 4060.0 17 5382.0 18 6584.0 19 7557.5 20 7729.0 21 8035.5 22 10061.0 23 14794.0 24 17127.0 25 21341.5 26 42289.5 27 55661.5 28 49811.0 29 44505.0 30 45935.0 31 49063.5 32 53574.5 33 69111.5 34 72628.5 35 87681.0 36 170393.0 37 277278.5 38 303101.0 39 298613.5 40 382686.0 41 516152.0 42 579338.5 43 560435.5 44 621301.5 45 773220.0 46 1307789.5 47 1447474.0 48 1192120.0 49 1199647.5 50 1023468.0 51 832463.5 52 671399.5 53 661135.5 54 609804.0 55 360132.0 56 218072.0 57 142293.5 58 70369.5 59 38044.5 60 19769.5 61 15158.0 62 14717.5 63 12566.5 64 8422.0 65 6076.5 66 4831.0 67 3549.5 68 2210.0 69 1366.0 70 1018.5 71 639.0 72 387.0 73 270.5 74 328.5 75 423.5 76 377.0 77 246.0 78 132.5 79 90.5 80 101.5 81 124.5 82 130.0 83 79.5 84 63.0 85 62.0 86 42.5 87 35.5 88 24.0 89 13.0 90 6.0 91 6.5 92 8.0 93 6.5 94 3.0 95 3.0 96 5.0 97 5.5 98 6.5 99 4.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01019789300753826 2 0.01129551358564903 3 0.011887563473236051 4 0.01255278806603046 5 0.011129207437450429 6 7.916172654253443E-4 7 8.780964624886173E-4 8 0.0013437536774447022 9 0.0016497569901301292 10-14 0.005610504215628028 15-19 0.009081646140829245 20-24 0.01629667207427738 25-29 0.020290680529415 30-34 0.02689769118504905 35-39 0.034781933058848365 40-44 0.043638733287313096 45-49 0.049511335992502126 50-54 0.057084252756873656 55-59 0.05449519864171782 60-64 0.062121333373512895 65-69 0.058660835041796394 70-74 0.05966798507528713 75-79 0.05722394992136048 80-84 0.05015926474588388 85-89 0.05111851860869341 90-94 0.04585792252887524 95-99 0.04370126439903577 100-104 0.032384463626417316 105-109 0.02681520333554254 110-114 0.02709725856288737 115-119 0.02446429962460711 120-124 0.02232360688499471 125-129 0.02270677625044429 130-134 0.020860112780847012 135-139 0.0217062784628815 140-144 0.022641584240350433 145-149 0.01856508793570631 150-151 0.014508548368846014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 1.5032517E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.322594022176648 #Duplication Level Percentage of deduplicated Percentage of total 1 80.85801922417143 5.112324289917907 2 7.528833625038929 0.9520351694326735 3 3.5557400965617276 0.6744450323680498 4 1.8931888808505133 0.4787945880366701 5 1.0064224203222714 0.3181600189257073 6 0.8808542228219659 0.33415701861739305 7 0.4800683293030495 0.21246940043614534 8 0.3503488753842101 0.17720909641444144 9 0.27705528668909507 0.15765372894896185 >10 1.9073293893327556 2.4625374369489963 >50 0.33367195956292695 1.4988037332256248 >100 0.519554501940883 7.635660565550048 >500 0.15146832657260356 6.815099729242317 >1k 0.1991033652719677 27.81396992087953 >5k 0.0362124448361949 15.794099419602603 >10k+ 0.0221290513396359 29.562580851452736 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC 298072 1.9828482482341445 No Hit CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG 216617 1.4409895561734605 No Hit GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG 100007 0.6652711585159026 No Hit CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT 92241 0.6136098166394889 No Hit CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC 76339 0.5078258018933224 No Hit CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG 69825 0.46449307191869466 No Hit CTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTG 54804 0.3645696858350468 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 49164 0.3270510188014423 Illumina Single End PCR Primer 1 (100% over 50bp) CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC 48495 0.32260066627564765 No Hit GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG 43270 0.28784268130213986 No Hit GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA 41148 0.2737266154430426 No Hit CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG 40771 0.2712187187282077 No Hit CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC 40532 0.269628831951429 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 40183 0.26730719812257653 No Hit CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA 39761 0.26449995034098417 No Hit GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA 35277 0.23467127960008294 No Hit ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA 35179 0.2340193594991444 No Hit CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT 34147 0.22715424170150614 No Hit CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG 33440 0.2224511038304497 No Hit CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC 32989 0.2194509409169469 No Hit CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG 32469 0.215991773034416 No Hit CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC 32017 0.21298495787498528 No Hit GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC 32012 0.21295169664534555 No Hit CCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATT 31653 0.21056354035721364 No Hit CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA 31143 0.20717089493396215 No Hit CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG 30668 0.20401107811818872 No Hit CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA 30076 0.20007294852884583 No Hit GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT 29476 0.19608160097207938 No Hit CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC 28888 0.19217008036644828 No Hit CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA 28438 0.18917656969887345 No Hit CTCAGAATGAGGAGGTAGTGACGAAAAATAACGAGACCGTTCTCTTTGAG 26930 0.17914498283953378 No Hit CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT 25981 0.17283200145391486 No Hit CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG 25603 0.170317452493152 No Hit CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA 25592 0.17024427778794463 No Hit CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT 25510 0.16969879362185322 No Hit CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT 25086 0.16687824134840493 No Hit CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA 24918 0.16576066403251033 No Hit GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA 24902 0.16565422809766323 No Hit CTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAAACTAACGAGGATTT 24075 0.16015282071525347 No Hit CTCACGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTGCCC 23956 0.15936120344982813 No Hit CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA 23869 0.158782458054097 No Hit CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG 23199 0.1543254532823745 No Hit CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC 22873 0.1521568211098647 No Hit CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC 22228 0.1478661224863408 No Hit CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCTCATAGATCCTC 22167 0.1474603354847362 No Hit CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC 22004 0.1463760193984813 No Hit CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC 21798 0.14500565673732482 No Hit GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT 21629 0.14388142717550229 No Hit CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC 21451 0.14269732740032823 No Hit CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG 21266 0.14146666190365859 No Hit CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT 21024 0.13985681838909614 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG 20983 0.1395840763060504 No Hit GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC 20958 0.1394177701578518 No Hit GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG 20810 0.13843323776051608 No Hit CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC 20804 0.1383933242849484 No Hit CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT 20797 0.1383467585634528 No Hit CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC 20782 0.13824697487453363 No Hit CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA 20570 0.13683669873780951 No Hit GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC 20472 0.136184778636871 No Hit CATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTGCA 20353 0.13539316137144564 No Hit GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT 20108 0.13376336111909934 No Hit CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA 19842 0.13199386370226623 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG 19688 0.13096941782936286 No Hit ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC 19241 0.12799586389957185 No Hit GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA 19194 0.12768320834095848 No Hit CTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAATATG 19191 0.12766325160317463 No Hit CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC 19113 0.127144376420795 No Hit CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC 18807 0.12510878916684412 No Hit GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG 18577 0.12357877260341697 No Hit CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT 18515 0.12316633335588444 No Hit CTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGGAAG 18372 0.12221506218818845 No Hit CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT 18074 0.12023269290166111 No Hit CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA 17887 0.11898872291313557 No Hit CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT 17879 0.11893550494571203 No Hit CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG 17501 0.11642095598494917 No Hit ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG 17476 0.11625464983675057 No Hit CTCGTTTAAAATTCGGAGCTTTGGTGCATGTATTAGCTCTAGAATTGCCA 17362 0.11549629380096493 No Hit CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT 17273 0.11490424391337792 No Hit CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGC 17088 0.11367357841670826 No Hit GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA 16982 0.11296844034834619 No Hit AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG 16891 0.11236308596890329 No Hit CTGAATACTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGT 16783 0.11164464340868531 No Hit GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT 16536 0.11000153866448313 No Hit CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG 16516 0.10986849374592425 No Hit CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG 16345 0.10873095969224582 No Hit TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT 16264 0.10819212777208236 No Hit CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT 16216 0.10787281996754103 No Hit GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC 16202 0.10777968852454982 No Hit CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA 16188 0.1076865570815586 No Hit CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT 16034 0.10666211120865521 No Hit CTCGCACCGAAGACAGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATC 15931 0.10597692987807698 No Hit GTCGTGATTACCCGCTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAA 15864 0.10553122940090473 No Hit GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG 15440 0.10271067712745642 No Hit GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG 15404 0.10247119627405045 No Hit CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA 15369 0.1022383676665724 No Hit CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT 15298 0.10176605820568838 No Hit CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT 15293 0.10173279697604865 No Hit GTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTGCAATCT 15253 0.10146670713893088 No Hit GTTTTATTTGATATTTCGGCCGGCGTTAAACAGTCAACTTAGAACTGGCA 15102 0.10046221800381135 No Hit GTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCTCGTGCGCTTTGTTCGA 15045 0.10008303998591854 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.585723468664629E-4 0.0 0.0 2 7.450515439297358E-4 0.0 0.0 3 7.849650194974002E-4 0.0 0.0 4 9.712279054798342E-4 0.0 0.0 5 0.0010044891351195546 0.0 0.0 6 0.001051054856615163 0.0 0.0 7 0.001150838545534324 0.0 0.0 8 0.0012639267263093732 0.0 0.0 9 0.0018426721220405073 0.0 0.0 10-11 0.0025311795755827184 0.0 0.0 12-13 0.0028305306423402014 0.0 0.0 14-15 0.0034525156366029723 0.0 0.0 16-17 0.0040944573686495745 0.0 0.0 18-19 0.004623310919921128 0.0 0.0 20-21 0.0053051661275353955 0.0 0.0 22-23 0.00613004462260046 0.0 0.0 24-25 0.00743721094744147 0.0 0.0 26-27 0.01039746038537658 0.0 0.0 28-29 0.013530668217438237 0.0 0.0 30-31 0.018333589777413853 0.0 0.0 32-33 0.024483591137798147 0.0 0.0 34-35 0.03320135942636885 0.0 0.0 36-37 0.04410106437930521 0.0 0.0 38-39 0.05692659452838138 0.0 3.326122963972035E-6 40-41 0.07231656548267998 0.0 6.65224592794407E-6 42-43 0.09053041483339083 0.0 6.65224592794407E-6 44-45 0.11135527071081974 0.0 6.65224592794407E-6 46-47 0.13643756398213286 0.0 6.65224592794407E-6 48-49 0.16535155090794176 0.0 6.65224592794407E-6 50-51 0.20029579876743198 0.0 6.65224592794407E-6 52-53 0.24408753371108777 0.0 6.65224592794407E-6 54-55 0.29387959448174916 0.0 6.65224592794407E-6 56-57 0.3482650310656559 0.0 6.65224592794407E-6 58-59 0.40089760084754933 0.0 6.65224592794407E-6 60-61 0.4648589454447316 3.326122963972035E-6 6.65224592794407E-6 62-63 0.5326918971719772 6.65224592794407E-6 6.65224592794407E-6 64-65 0.601592534370658 6.65224592794407E-6 6.65224592794407E-6 66-67 0.6787985006103768 6.65224592794407E-6 6.65224592794407E-6 68-69 0.7668941934341401 6.65224592794407E-6 6.65224592794407E-6 70-71 0.8647686877719812 6.65224592794407E-6 6.65224592794407E-6 72-73 0.9641199807058259 6.65224592794407E-6 6.65224592794407E-6 74-75 1.0777070799254709 6.65224592794407E-6 6.65224592794407E-6 76-77 1.2089592182067714 6.65224592794407E-6 6.65224592794407E-6 78-79 1.3399785278805938 6.65224592794407E-6 6.65224592794407E-6 80-81 1.497793084152175 6.65224592794407E-6 6.65224592794407E-6 82-83 1.684771086571863 6.65224592794407E-6 6.65224592794407E-6 84-85 1.869234540030788 6.65224592794407E-6 6.65224592794407E-6 86-87 2.062315978089365 6.65224592794407E-6 6.65224592794407E-6 88-89 2.2800639440487576 6.65224592794407E-6 6.65224592794407E-6 90-91 2.5220327374317955 6.65224592794407E-6 6.65224592794407E-6 92-93 2.79262947116574 6.65224592794407E-6 6.65224592794407E-6 94-95 3.1008845690977767 6.65224592794407E-6 6.65224592794407E-6 96-97 3.421516170578753 6.65224592794407E-6 6.65224592794407E-6 98-99 3.7934565449019617 6.65224592794407E-6 6.65224592794407E-6 100-101 4.177251221468767 6.65224592794407E-6 6.65224592794407E-6 102-103 4.5692514433876905 6.65224592794407E-6 6.65224592794407E-6 104-105 5.028921637008626 6.65224592794407E-6 6.65224592794407E-6 106-107 5.539807472028802 6.65224592794407E-6 6.65224592794407E-6 108-109 6.070437172963118 6.65224592794407E-6 6.65224592794407E-6 110-111 6.6330209372123115 6.65224592794407E-6 6.65224592794407E-6 112-113 7.3932296234888675 6.65224592794407E-6 6.65224592794407E-6 114-115 8.192546863575807 6.65224592794407E-6 1.330449185588814E-5 116-117 9.00700794151771 6.65224592794407E-6 1.330449185588814E-5 118-119 9.934510634513169 6.65224592794407E-6 1.330449185588814E-5 120-121 10.885432559297954 6.65224592794407E-6 1.330449185588814E-5 122-123 11.954119193745132 6.65224592794407E-6 1.330449185588814E-5 124-125 13.348173828773984 6.65224592794407E-6 1.330449185588814E-5 126-127 15.101316698993255 6.65224592794407E-6 1.330449185588814E-5 128-129 16.5683697547124 6.65224592794407E-6 1.330449185588814E-5 130-131 18.86835384919239 6.65224592794407E-6 1.330449185588814E-5 132-133 21.3498378215704 6.65224592794407E-6 1.330449185588814E-5 134-135 22.732942859801852 6.65224592794407E-6 2.660898371177628E-5 136-137 24.161469433229314 6.65224592794407E-6 2.660898371177628E-5 138-139 25.40662684765299 6.65224592794407E-6 2.660898371177628E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCAAT 4495 0.0 114.63962 1 TCCAATC 4620 0.0 112.47952 2 CAACCAG 39405 0.0 110.98326 3 CTTGTTA 10980 0.0 109.04418 1 CCAGACG 40625 0.0 108.99861 6 TGGCTCA 29480 0.0 108.421295 3 CAGACGT 41480 0.0 106.75226 7 ACCTGGC 13965 0.0 98.50707 4 CACCTGG 14485 0.0 95.068924 3 GTTACGA 12715 0.0 94.28254 4 CCTGGCC 14615 0.0 93.97533 5 GCGTCGG 3320 0.0 93.450455 145 TTACGAC 12980 0.0 92.35583 5 ACCCTCA 2110 0.0 91.3698 3 CTCAACC 50265 0.0 89.26679 1 CAAAGGA 5950 0.0 86.72395 8 ATCAAAG 6225 0.0 86.617714 6 CTAGCAT 3485 0.0 85.47397 1 TACGCAA 3795 0.0 77.915596 9 GGGGTCC 820 0.0 76.9097 145 >>END_MODULE