FastQCFastQC Report
Fri 3 Apr 2015
H2V3NBCXX l02n02 library 2 l4b.34200000004533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2V3NBCXX l02n02 library 2 l4b.34200000004533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22705422
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT3905421.7200385000551852No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG2670381.1760979381929126No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC2129210.9377539866909322No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC2001870.8816704661996593No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA1915960.8438336887110048No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC1908270.840446832479044No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC1783720.7855920933775202No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT1747880.7698073173887717No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC1741830.7671427555937961No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG1494790.6583405496713516No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC1130880.4980660566449723No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT1031150.45414262725440646No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG1001050.440885881795106No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA978680.4310336095052538No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA904150.39820885073177675No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC887950.3910739910493626No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG872630.3843267039916721No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA857740.3777687990119717No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG805960.3549636734344775No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA792580.34907080784492794No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA776270.3418875015844233No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC745130.3281727157504494No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG743060.3272610392354742No Hit
CCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATT706410.3111195202626051No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT672890.2963565266481284No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC654580.2882923735132516No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA649570.28608585209294946No Hit
CTGAAGTTTCCCTCAGGATAGCTGGAGTACGTTAAATAAAGCAGTTATAT636570.28036034740953064No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT628080.276621152427821No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT617830.2721068121966639No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC613800.27033190574480404No Hit
GTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTA595900.2624483262191736No Hit
CCCACTTGGAGCACACATTCAATGTCTATGCTTCATAAAAAATTTAAGCA572440.25211599238278853No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG570450.2512395497427883No Hit
CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG569530.2508343601805771No Hit
CTCAGAATGAGGAGGTAGTGACGAAAAATAACGAGACCGTTCTCTTTGAG566390.2494514305878129No Hit
CTCTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGG563570.24820943649494823No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT559480.2464081046368572No Hit
CTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATC550300.2423650174834892No Hit
CTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAATATG549280.24191578557755938No Hit
CTGAGATAATGGTTAAGAGGGACGGACGGGGGCATTCGTATCGCTGCGTG525980.23165391949112418No Hit
CCCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCA523680.23064094558559625No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTT523350.23049560585132484No Hit
CTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCC517110.22774736360328382No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG512570.22574784119845911No Hit
CTCGTTATTTTTCGTCACTACCTCCTCATTCTGAGAGTGGGTAATTTGCG510400.22479212233976537No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT509940.22458952755865977No Hit
CTGTTATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAA509850.22454988944931303No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA508740.2240610194340365No Hit
GCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAA505530.22264726020066924No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT504310.22210994360730227No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAAC488780.21527016762780274No Hit
CTGCTTACCAAAAATGGCCCACTTGGAGCACACATTCAATGTCTATGCTT487840.21485616959684783No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA485750.21393568461312898No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT478900.21091878406840447No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT475020.20920994113212255No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG466730.2055588308378501No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG464240.20446217647925682No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC463480.20412745466699542No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC460960.20301758760528651No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG460400.20277095048046234No Hit
CTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAA455880.20078023654438132No Hit
CTAGCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAA441190.19431041625211812No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT434040.19116138867623778No Hit
CTCCGGAGTCGAACCCTTATTCTCCGTTACCCGTTACAACCATGGTAGGC432580.19051837045794612No Hit
CTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAAAGAGGGTGGTAAACCTC429680.18924114249010654No Hit
CTCATGGTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTG421950.18583666932065832No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA416230.18331744725995403No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTT414090.18237494110437585No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC408750.1800230799498023No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA399440.17592273774960007No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA399290.17585667423402215No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG394490.17374264173552909No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG392060.17267241278316695No Hit
GTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAA391270.17232447826778996No Hit
CCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTC390980.172196755471006No Hit
CTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACAACTTC381590.1680611793958289No Hit
CCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAGG372510.16406213458617946No Hit
GTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGA366020.16120378647884193No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG365240.16086025619783678No Hit
ATCCGTTTCCATCTCAGCGGTTTCACGCCCTCTTGAACTCTCTCTTCAAA364670.16060921483864074No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC353960.15589227982637802No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA352760.15536377170175475No Hit
CTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTT352360.15518760232688034No Hit
ATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGACGGACGGGG350950.154566605280448No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC348640.1535492271405482No Hit
CTTCCGTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGA348420.15345233398436725No Hit
AAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAGC347130.15288418775039725No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG347070.1528577623441661No Hit
CTAATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGG346480.1525979125162263No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT344420.151690640235623No Hit
CTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTCA341820.15054553929893924No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC337650.14870897356587337No Hit
CTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCG332530.14645400556748076No Hit
CTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTA331720.14609726258336006No Hit
CTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGGTC330420.14552471211501816No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT330130.14539698931823422No Hit
CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG325080.1431728509604446No Hit
AACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGAATAGC323770.14259589625773086No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG321730.1416974324458713No Hit
CGGAGAATAAGGGTTCGACTCCGGAGAGGGAGCCTGAGAAACGGCTACCA321280.14149924189913757No Hit
CCATGCACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTT319930.14090467025893638No Hit
CTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCA319030.14050828916546892No Hit
CTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTATCAACTTTCGATG316650.1394600813849661No Hit
AAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGC314750.13862327685431258No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTAC313680.1381520237765235No Hit
CTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTCAT313430.138041917917227No Hit
CTCAGATCAGAAAACCAACAAAATAGAACCGAGATCCTATTTCATTATTC311530.13720511338657349No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA310540.1367690941837593No Hit
CGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAACGG309210.13618333101230182No Hit
GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC308980.13608203362174903No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA308070.1356812482939097No Hit
CCCCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAA301640.13284932559280332No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC299710.13199930835903426No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC298340.13139592825008933No Hit
CGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGC296320.1305062729069735No Hit
GTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTT294840.1298544462199381No Hit
CTCTTGCTAGAAACCTTCTAAAAAATTCAAAAGGATCGATAGGCCACGCT288270.1269608642376257No Hit
GGTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGT287810.12675826945652013No Hit
ATCACTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAG286460.12616369781631892No Hit
GTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAACTAG286320.1261020385351129No Hit
GTGGCTAGCAAGTTTACCTTGAAAAAATTAGAGTGCTCAATGCGGGCTAA283830.1250053841765196No Hit
GTAACTATTTAGTAGGCTAGAGTCTCGCTCGTTATCGGAATAAACCAGAC281790.12410692036466002No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA281070.12378981548988607No Hit
AGGTGCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGG281030.12377219855239863No Hit
CAAGTGTCTGCCTTATCAACTTTCGATGGTAGTTTATGTGCCTACCATGG280170.12339343439641863No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG278800.12279005428747372No Hit
CTCCTCATTCTGAGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT276100.12160091100707135No Hit
CTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAA275540.12135427388224715No Hit
GTAACATAACCCCGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTA274530.12090944621068925No Hit
CTCGTAGTTGGATCTGCGTCTCAGGACCTGGTCCATCCATTGGATGAGAA274040.12069363872646806No Hit
CCCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAAT272410.11997574852385477No Hit
ATCCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAA271960.11977755797712106No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT265290.11683993365109004No Hit
CACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAAT264610.11654044571380352No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA262830.11575649199561233No Hit
CTCAGGATAGCTGGAGTACGTTAAATAAAGCAGTTATATTCGGTAAAGCG260440.11470387998073764No Hit
ATCAAGTGTCTGCCTTATCAACTTTCGATGGTAGTTTATGTGCCTACCAT260270.11462900799641602No Hit
CTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTG260150.11457615718395368No Hit
GTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGT257320.11332975885671713No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT254490.11208336052948058No Hit
GTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTGCAATCT254400.11204372242013384No Hit
GTCAGAATCGCTTCGGACTTTCACCAGAGTTTCCTCTGGCTTCATCCTGC252590.11124655599882706No Hit
CGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGC252390.11115847131138985No Hit
GTAAAAATATGTTACGGATAGTTATACCTATACGCTACATTTCCATATAG249600.10992968992164073No Hit
CTCAGGACCTGGTCCATCCATTGGATGAGAACTAGGCTCCTAGGCTAATA246400.10852033492264535No Hit
CTCCAGCTATCCTGAGGGAAACTTCAGAGGGAACCAGCTACTAGATAGTT246330.10848950528204232No Hit
ATTTTAATCCGTTAATTGGTGACTCTGAATAGCTATGGCTGATCGCATGG246320.10848510104767045No Hit
CACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGA244030.10747653137651439No Hit
CTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGT242740.10690838514254436No Hit
CTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGG234590.10331893412947797No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC234500.10327929602013122No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA234110.10310753087962868No Hit
CTCTCATAGATCCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACC233400.10279483023922656No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG230990.10173340975560814No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA230690.10160128272445233No Hit
CTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAGTGAGATC227310.10011265150676346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGC520250.0112.865614
CACCTGG524950.0111.948543
CCTGGCC537150.0109.259665
CTCCTCG12400.0106.966131
TGGCCCG570800.0102.741247
GCCCGGA576900.0101.632679
CTGGCCC582350.0100.715986
TTAGATT267750.099.6990662
CCCTCGT18000.099.458371
GTCCTCG9350.096.898121
GTAGGGG61650.095.36139145
AGATTAA278900.095.0930944
CTCCCAC126450.094.633551
TAGATTA281050.094.569253
CCTCAAC116750.090.266051
TGGCTCA255400.089.67863
CCAGACG318650.088.859216
CAGACGT319850.088.457847
AGAACCC15700.085.8812145
ACTTATG141050.084.469836
Produced by FastQC (version 0.11.2)