FastQCFastQC Report
Fri 3 Apr 2015
H2V3NBCXX l02n01 library 3 l3b.341000000045bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2V3NBCXX l02n01 library 3 l3b.341000000045bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17530766
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC3032971.7300841275275707No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG2255181.2864126986807078No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1452440.8285091478603959No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1162280.6629944179278874No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1114020.6354656721788426No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC741300.4228565939446115No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG667750.38090178147378156No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC663100.3782493018274273No Hit
CTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTG652180.37202025285147267No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG600540.34256346813367994No Hit
CTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAAACTAACGAGGATTT595620.33975697354011797No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC587270.3349939186912882No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC582430.33223305815615817No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT538060.30692326849836454No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG459650.2621961869777966No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA458270.26140899946984636No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT458090.26130632283837457No Hit
CTCAGAATGAGGAGGTAGTGACGAAAAATAACGAGACCGTTCTCTTTGAG445580.25417029695108584No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA442610.2524761325318015No Hit
GTCGTGATTACCCGCTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAA424560.24217994809810364No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT420570.23990394943381255No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA419330.23919662152811805No Hit
CTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAATATG417980.23842654679207972No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA414570.2364813950514199No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA393380.22439407382426985No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT389680.22228349862179442No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA381110.21739495011227689No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC376500.21476528749513857No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT376470.21474817472322658No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG375830.21438310225577137No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA372690.2125919654623192No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG371170.2117249183521131No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC359830.20525629056939096No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC357700.2040412837636416No Hit
ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA344790.19667708758419342No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC340300.1941158760547029No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC330910.18875957844625843No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT327340.1867231585887348No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA319860.18245637412535196No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA308460.17595352079880594No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG303980.17339801352661943No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT302560.17258800898945317No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG301910.17221723226469396No Hit
CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA298800.1704432082431538No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT297290.16958186539025163No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT296310.16902284817446084No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC296090.1688973545137731No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA295940.16881179065421328No Hit
CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG292640.16692938574389732No Hit
GCTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAAACTAACGAGGATT289920.16537782775721266No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC285800.1630276737479697No Hit
CTCAGGACCTGGTCCATCCATTGGATGAGAACTAGGCTCCTAGGCTAATA283950.16197238614673198No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG283480.16170428605344456No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT282470.1611281560657418No Hit
GTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTGCAATCT279010.15915448303856203No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG277350.15820757632610008No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA274660.15667313111132736No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT269620.15379818543011756No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC267620.15265733396932No Hit
CTGAAGTTTCCCTCAGGATAGCTGGAGTACGTTAAATAAAGCAGTTATAT266080.15177887834450587No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC261020.1488925241486881No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC259470.14800836426657No Hit
CCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATT255940.14599476143826232No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA254090.14493947383702457No Hit
CTCTTTTCTGGTTAAGGAACGGACTCCCTGGAATCGGTTCAGCCGGAGAT254070.1449280653224166No Hit
CTCAGATCAGAAAACCAACAAAATAGAACCGAGATCCTATTTCATTATTC251230.14330805624808407No Hit
CTGAATACTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGT250680.14299432209636476No Hit
CTCAGTCGTGATTACCCGCTGAATTTAAGCATATAACTAAGCGGAGGAAA250070.1426463624008215No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA249420.1422755856760623No Hit
CTCACGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTGCCC248050.14149410242541596No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG244890.13969155711735587No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC243950.13915535693078102No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG242510.13833394387900677No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG242440.13829401407787886No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG240930.1374326712249767No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG239950.13687365400918589No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA235980.13460906385950278No Hit
CTTCCGTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGA231340.13196228847045247No Hit
GGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTTCAA228720.13046777305680768No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG228370.1302681240511681No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA227240.12962354297581746No Hit
CTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATC225540.12865381923413957No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG222940.12717071233510274No Hit
CTCAATGCGGGCTAATGCCTGAATACTCGTGCATGGAATAATGAAATAGG222470.12690261224181532No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC221420.1263036652248966No Hit
CTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAG220250.12563626712033005No Hit
CTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTTCAAATAAACCTATAA219510.12521415207983497No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT218510.1246437263494362No Hit
CTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGGAAG218390.12457527526178833No Hit
CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGC216100.12326900033917514No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA215320.12282406826946408No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT213940.12203688076151378No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC213890.12200835947499383No Hit
CGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAGCCCAACGCTGA213130.12157483591989078No Hit
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGC211630.12071919732429262No Hit
CTTTTACTCTACGAGAGGTTTCCGTCCTCTCTGAGCTAGCCTTAGGACAC210130.11986355872869445No Hit
CGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAGAA208600.11899080736118434No Hit
GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT208530.11895087756005641No Hit
CATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTGCA206220.11763319412283525No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT206000.11750770046214751No Hit
CTCAAAGGCTAAGCCATGCATGTCTAAGTTCAAATAAACCTATAATGGTG203740.1162185383114463No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA203160.11588769138781499No Hit
CTCAGGTATAGTTCACCATCTTTCGGGTCCCAGCATATACGCTCTACCTC203080.11584205732938309No Hit
CTTCAACCTATTCTCAAACTTTCAATGAGTATGACGTCTTGCTTGCTTAA202730.11564240832374352No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT201690.11504916556412881No Hit
CTAGCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAA200880.11458712072250579No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA199290.11368014381117175No Hit
CTCTCATAGATCCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACC198280.11310401382346898No Hit
CTTTCATCGATTCTTTACTTATTCGGTAAAACGAGAATTGAAATATTATA195960.11178062612894382No Hit
GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA193150.11017772982652325No Hit
CTCGTAGTTGGATCTGCGTCTCAGGACCTGGTCCATCCATTGGATGAGAA191150.10903687836572572No Hit
CTCGTTATTTTTCGTCACTACCTCCTCATTCTGAGAGTGGGTAATTTGCG188220.1073655309756573No Hit
CTCGTTTAAAATTCGGAGCTTTGGTGCATGTATTAGCTCTAGAATTGCCA186050.10612770714069197No Hit
CTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAAAGAGGGTGGTAAACCTC184510.10524925151587786No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT184380.10517509617092602No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCTCATAGATCCTC183150.10447347252253553No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA180420.10291621027854686No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTT180410.10291050602124288No Hit
CTGCTTACCAAAAATGGCCCACTTGGAGCACACATTCAATGTCTATGCTT180330.10286487196281098No Hit
CTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGT177900.10147873743794197No Hit
CTCATCGTGAGAATTTAGCGCGGTAACGCAAATGAACTCGGAAACATTGA177250.10110796071318275No Hit
CTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGC175500.10010971568498489No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCAG383950.0117.70713
CCAGACG390550.0116.435676
CAGACGT396300.0114.759317
ACCTGGC153950.0109.643664
TGGCTCA301500.0109.3978653
CTTGTTA101200.0108.27791
CACCTGG158800.0106.4319463
CCTGGCC158700.0106.361945
AGTCGTG33100.0104.927124
CTAGCAT63350.0102.86631
GTTACGA113900.095.869724
TACGCAA69450.093.937229
TTACGAC118950.091.738635
ATACGCA71500.091.142548
GTCCAAT26650.090.94791
TGGCCCG192400.087.709257
TCCAATC28150.086.544822
CTGGCCC199650.084.468956
GCCCGGA199800.084.388199
ACCCTCA16350.081.154673
Produced by FastQC (version 0.11.2)