FastQCFastQC Report
Fri 3 Apr 2015
H2V3NBCXX l02n01 library 3 l3a.34100000004593.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2V3NBCXX l02n01 library 3 l3a.34100000004593.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15032517
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC2800051.8626621210539793No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG2342451.5582553473912586No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1014760.6750433077840524No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT841100.5595204049993757No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC807290.5370291615169968No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG684420.45529301580034803No Hit
CTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTG585120.38923621373586337No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC499260.33212003019853564No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC464520.30901012784485793TruSeq Adapter, Index 9 (100% over 50bp)
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG433970.2886875165349888No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG421260.28023251196057186No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA416570.27711260862036613No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA407720.27122537097413557No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC403360.268324991749552No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA386590.2571691753283898No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT364570.24252092979505693No Hit
ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA342250.2276731168838858No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA339280.2256973998432864No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG330630.21994320711561477No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA321470.213849749845618No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG317690.21133520088485513No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC311050.2069181095887003No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA310670.20666532424343842No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT298850.1988023695566085No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC294210.19571572744604246No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC292830.1947977175079862No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG288770.1920969056612409No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA282090.18765320538137426No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA278330.18515196091246727No Hit
CCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATT277330.18448673631967288No Hit
CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC274730.18275715237840742No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC272510.18128035378240384No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG269620.179357854709228No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC268830.1788323272809204No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT267790.17814049370441426No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA264010.1756259447436514No Hit
CTCAGAATGAGGAGGTAGTGACGAAAAATAACGAGACCGTTCTCTTTGAG263350.17518689651240707No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT262370.17453497641146856No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT248330.16519522312863508No Hit
CTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAAACTAACGAGGATTT242510.16132361599857165No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG241390.16057856445464191No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC239640.15941442141725168No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC239030.1590086344156471No Hit
CTCACGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTGCCC230730.1534872702954535No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA229670.15278213222709144No Hit
CATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTGCA223880.14893048183481183No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCTCATAGATCCTC220740.1468416766134374No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC219490.14601014587244437No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC218350.14525178983665876No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT214130.14244454205506635No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG212040.14105422265612605No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT207560.1380740164804071No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT204780.13622469211243865No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC204320.13591868879975325No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC203300.13524015971510295No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG202240.13453502164674086No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC200410.1333176606419271No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG199580.13276552422990773No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG199570.1327588719839798No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG199140.13247282540907818No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT197740.13154151097916603No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT197250.13121555092869677No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC196510.13072328473002892No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA195280.1299050584808918No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA194830.1296057074141343No Hit
CTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAATATG192080.1277763397839497No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC191380.1273106825689936No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG190360.1266321534843433No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT187270.12457660949260858No Hit
CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT184100.12246784753345032No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG182910.12167623026802497No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC182840.12162966454652936No Hit
CTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGGAAG181980.12105757139672618No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT181490.12073161134625691No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA180740.12023269290166111No Hit
GTCGTGATTACCCGCTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAA173600.11548298930910905No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA173500.1154164668498296No Hit
CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGC170290.11328109590695956No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG168620.1121701708369929No Hit
CTCGTTTAAAATTCGGAGCTTTGGTGCATGTATTAGCTCTAGAATTGCCA167150.11119229068558513No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC166910.11103263678331446No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT166440.11071998122470109No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG164430.10938287979318433No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA164230.10924983487462545No Hit
CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA163800.10896378829972385No Hit
CTGAATACTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGT163490.1087575686759576No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT162250.10793269018089253No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA161430.10738720601480112No Hit
CTCGCACCGAAGACAGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATC161410.10737390152294522No Hit
GTTTACGGTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACT157680.10489261379182209No Hit
GTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTGCAATCT157200.10457330598728078No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG156910.10438039085537039No Hit
GTTTTATTTGATATTTCGGCCGGCGTTAAACAGTCAACTTAGAACTGGCA156890.1043670863635145No Hit
GCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGT156370.10402116957526142No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT155530.10346238091731412No Hit
CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG154320.10265745916003288No Hit
GTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCTCGTGCGCTTTGTTCGA153270.10195897333759875No Hit
GGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTTCAA152860.10168623125455303No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT152430.10140018467965145No Hit
GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT152350.1013469667122279No Hit
CCCGGTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATT151920.10106092013732632No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA151470.10076156907056882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT43550.0114.618741
CAACCAG366800.0111.683323
CTTGTTA106900.0111.374621
CCAGACG377150.0110.1734166
TGGCTCA314650.0110.1461643
CAGACGT384250.0108.0954367
TCCAATC47050.0107.717612
ACCTGGC127200.097.414624
GTTACGA123300.096.4383854
TTACGAC124600.095.432215
CTAGCAT32400.095.393751
CACCTGG132400.093.588673
CCTGGCC133050.093.185945
ACCCTCA20600.092.2153553
CAAAGGA55850.090.0747158
ATCAAAG59400.087.639236
TACGCAA36650.084.058499
AGTCGTG18100.082.920324
ATACGCA38500.080.019318
CTCAACC548950.078.546761
Produced by FastQC (version 0.11.2)