FastQCFastQC Report
Fri 3 Apr 2015
H2V3NBCXX l02n01 library 1 l4a.3410000000450f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2V3NBCXX l02n01 library 1 l4a.3410000000450f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26150211
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10796134.128505884713511TruSeq Adapter, Index 5 (100% over 50bp)
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2255130.8623754508137621No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC1318430.5041756642040097No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG1295580.49543768499611723No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC1238830.4737361392609796No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC1137660.4350481149081359No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1060690.405614317987721No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG931690.3562839320875843No Hit
CCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATT861910.3295996349704406No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA822300.3144525296564529No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC809000.30936652862953956No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG805640.3080816441595825No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA764340.29228827254969375No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG754230.28842214695705515No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT695430.2659366687328068No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT654460.2502694911333603No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT643340.24601713538755002No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC594060.2271721631615133No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA588670.22511099432429055No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC561800.21483574262555666No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT561740.2148127982600217No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA558400.21353556191190962No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA524810.2006905412732616No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC524770.20067524502957165No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG504360.19287033668676706No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC502690.19223171851271104No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG478860.18311898133441445No Hit
CGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAACGG469830.17966585432140492No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG468560.17918019858424852No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG466780.17849951574004508No Hit
GTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTA460520.17610565360256558No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT454340.173742383952466No Hit
GCGTAACATAACCCCGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCG451730.1727443040516958No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG440730.16853783703695546No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG440700.16852636485418798No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC413730.15821287254622918No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA406440.15542513213373307No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC395920.1514022200432723No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC394870.15100069364641075No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT394560.1508821477578135No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT394170.15073300938183634No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC394060.15069094471168892No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC390960.14950548582571668No Hit
CCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTC382290.14619002500591677No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA376220.14386882002596463No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT364780.13949409433063464No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC364770.13949027026971217No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT356240.13622834630282715No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC355900.13609832823146245No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG354220.13545588599648392No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC348130.13312703289468678No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATGC341290.13051137522370276TruSeq Adapter, Index 5 (98% over 50bp)
GGGAAAACTGGCAATATTAGCCTAGGAGCCTAGTTCTCATCCAATGGATG340970.13038900527418307No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT336820.12880201999134921No Hit
GCGGTGTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTT335810.1284157898381776No Hit
GCTAAATTGATTGGTTGAATACCGATAAGTTTTGTTAACTCTATCCGTTT335630.12834695674157273No Hit
CTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTT335120.1281519296345257No Hit
CTCATGGTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTG332760.12724945125681777No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC329220.1258957336902559No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT329170.12587661338564343No Hit
GTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAACTAG328640.1256739381567514No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA327850.12537183734387458No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC326080.12469497856059364No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG324830.12421697094528225No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC321510.12294738271901515No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA318950.1219684231228574No Hit
GCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAACGGC315430.12062235367814049No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT315170.12052292809415573No Hit
CGCCGACGGAACGCTAGTTGGTATCGTTTACGGTCAGAACTAGGGCGGTA314550.12028583631696127No Hit
GAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAG314080.12010610545360417No Hit
CACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGA311990.11930687672080352No Hit
GGTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGT309130.11821319529697102No Hit
CCCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCA306930.11737190189402295No Hit
GTGAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCA301050.11512335407159813No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC295570.11302776868607294TruSeq Adapter, Index 5 (98% over 50bp)
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA294070.11245415954769926No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT293630.11228590086710963No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA290080.11092835923962524No Hit
GTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCTGTAACCA288750.11041975913693393No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA283590.10844654370092846No Hit
GTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTGCAATCT281530.10765878715089526No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA281140.10750964877491812No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT276370.10568557171488979No Hit
CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG275610.10539494308478047No Hit
CGGGGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCG275430.10532610998817563No Hit
ATCACTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAG275070.10518844379496593No Hit
GTGGCTAGCAAGTTTACCTTGAAAAAATTAGAGTGCTCAATGCGGGCTAA273480.10458041810828983No Hit
GGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCT272440.10418271577235073No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT271400.10378501343641165No Hit
CCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAGG268370.10262632297689683No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG267340.10223244470188023No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA264240.10104698581590796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGC392850.085.24584
CCTGGCC401800.083.401115
CACCTGG407850.082.235053
CCTCAAC154950.080.166051
TGGCCCG430350.077.903067
CTGGCCC442100.075.8790056
CCCTCGT17750.075.2138441
GCCCGGA448000.074.963339
GGCGGTT77950.073.013864
AGTCGTG19150.068.526984
GGTTATA84450.067.123057
TGGCTCA287650.066.742953
CTTGTTA82600.066.056591
GGACCCT6600.065.9607541
CCAGACG297450.064.10396
CAGACGT302650.062.991177
CTCCCAC192700.061.3363151
GGCCGAC12600.061.0398751
CTGTCGG14950.058.2395941
TTACGAC93100.057.8616645
Produced by FastQC (version 0.11.2)