##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2V3NBCXX l01n02 library 3 mfb.34200000004576.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28369030 Sequences flagged as poor quality 0 Sequence length 151 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.60818392451205 38.0 38.0 38.0 32.0 38.0 2 36.56177292632142 38.0 38.0 38.0 32.0 38.0 3 36.679321605285764 38.0 38.0 38.0 32.0 38.0 4 36.566296591741065 38.0 38.0 38.0 32.0 38.0 5 36.57227804405015 38.0 38.0 38.0 32.0 38.0 6 38.73007695363571 40.0 38.0 40.0 38.0 40.0 7 38.77500855686641 40.0 38.0 40.0 38.0 40.0 8 38.81328011567544 40.0 40.0 40.0 38.0 40.0 9 38.80767608198095 40.0 40.0 40.0 38.0 40.0 10-14 38.8011160762282 40.0 38.8 40.0 38.0 40.0 15-19 38.759574613583894 40.0 38.0 40.0 38.0 40.0 20-24 38.71633709717956 40.0 38.0 40.0 38.0 40.0 25-29 38.68854943577556 40.0 38.0 40.0 38.0 40.0 30-34 38.62851586395446 40.0 38.0 40.0 38.0 40.0 35-39 38.568220365659315 40.0 38.0 40.0 38.0 40.0 40-44 38.44231069585389 40.0 38.0 40.0 38.0 40.0 45-49 38.311927654911 40.0 38.0 40.0 38.0 40.0 50-54 38.23779363622937 40.0 38.0 40.0 38.0 40.0 55-59 38.16458452756404 40.0 38.0 40.0 38.0 40.0 60-64 38.1148217757181 40.0 38.0 40.0 38.0 40.0 65-69 37.92276815950352 40.0 38.0 40.0 36.8 40.0 70-74 37.83602503857199 40.0 38.0 40.0 33.2 40.0 75-79 37.72705525708845 40.0 38.0 40.0 32.0 40.0 80-84 37.61337027032648 40.0 38.0 40.0 32.0 40.0 85-89 37.46775208739954 40.0 38.0 40.0 32.0 40.0 90-94 37.292588615120074 38.8 38.0 40.0 32.0 40.0 95-99 37.12199172830371 38.0 38.0 40.0 32.0 40.0 100-104 35.69974617390866 38.0 35.8 38.8 29.0 39.2 105-109 37.29177218960253 39.2 38.0 40.0 32.0 40.0 110-114 37.222521207105075 39.6 38.0 40.0 32.0 40.0 115-119 37.03673325453849 38.0 38.0 40.0 32.0 40.0 120-124 36.65393147386428 38.0 38.0 40.0 31.0 40.0 125-129 36.35351564716876 38.0 38.0 40.0 27.0 40.0 130-134 35.964553959017984 38.0 38.0 40.0 27.0 40.0 135-139 35.545845642237325 38.0 38.0 40.0 23.0 40.0 140-144 35.109470186326426 38.0 38.0 40.0 22.0 40.0 145-149 34.527971953923 38.0 32.0 40.0 18.4 40.0 150-151 29.36520557805466 30.0 22.5 39.0 13.0 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.08389159895558151 2201 2 0.22248548520970957 2201 3 0.2153297766575193 2201 4 0.2924895233239795 2201 5 0.28830536079038893 2201 6 0.25361541254867603 2201 7 0.19970895188326665 2201 8 0.2274646634840778 2201 9 0.20010953800162667 2201 10-14 0.19956876879248142 2201 15-19 0.2095365068225945 2201 20-24 0.21053774521737978 2201 25-29 0.20561150920809723 2201 30-34 0.16294221890958482 2201 35-39 0.208117171279099 2201 40-44 0.2079734781980349 2201 45-49 0.22351687522850483 2201 50-54 0.21990972667446584 2201 55-59 0.21447727017767448 2201 60-64 0.18756802960101737 2201 65-69 0.26233142998898273 2201 70-74 0.28612278260552415 2201 75-79 0.18718437717790692 2201 80-84 0.1712554324731883 2201 85-89 0.2510062449832162 2201 90-94 0.26936835360101696 2201 95-99 0.2200501869563425 2201 100-104 0.2502201304923659 2201 105-109 0.31179771446826265 2201 110-114 0.34423421984408265 2201 115-119 0.3574110441906626 2201 120-124 0.3387853206825824 2201 125-129 0.35966518920999846 2201 130-134 0.4472349665028048 2201 135-139 0.45151393889366176 2201 140-144 0.4835650581080486 2201 145-149 0.4198547051848607 2201 150-151 0.615988071982688 1101 1 0.08971688741065265 1101 2 0.18719583211846214 1101 3 0.18208608292764694 1101 4 0.23663769123117362 1101 5 0.24259509301216298 1101 6 0.23383457681633502 1101 7 0.15236696187193388 1101 8 0.14978155419214545 1101 9 0.12720576686778884 1101 10-14 0.16818853731307115 1101 15-19 0.19082244974915596 1101 20-24 0.19903716716214603 1101 25-29 0.18161064251557946 1101 30-34 0.13109010939658816 1101 35-39 0.17266344947388035 1101 40-44 0.19260626573059625 1101 45-49 0.2070932410913997 1101 50-54 0.1575657883735957 1101 55-59 0.16995579368047942 1101 60-64 0.15849545441233204 1101 65-69 0.24536969968556832 1101 70-74 0.2649436056841097 1101 75-79 0.2264271039140624 1101 80-84 0.18923507869398293 1101 85-89 0.2210765835359112 1101 90-94 0.20779175202473965 1101 95-99 0.2154269588181279 1101 100-104 0.39059509869981923 1101 105-109 0.324843560773715 1101 110-114 0.3355627717714498 1101 115-119 0.38281882220289276 1101 120-124 0.4119596687692493 1101 125-129 0.4562682339419126 1101 130-134 0.43757810563913324 1101 135-139 0.45200916784759926 1101 140-144 0.4303105324889316 1101 145-149 0.43262194584961833 1101 150-151 0.512619602922701 2202 1 8.669398059453215E-4 2202 2 0.14908631658804694 2202 3 0.20362857020399616 2202 4 0.20652860117581895 2202 5 0.24558675476188796 2202 6 0.2127894992655115 2202 7 0.20357579412878124 2202 8 0.1898492688208009 2202 9 0.17528290310750805 2202 10-14 0.17776687233178023 2202 15-19 0.18128413412983235 2202 20-24 0.19985490049954535 2202 25-29 0.18790806349843336 2202 30-34 0.19312442141929154 2202 35-39 0.20589335835797584 2202 40-44 0.21455293683897025 2202 45-49 0.1678972141075903 2202 50-54 0.19121022019145784 2202 55-59 0.16112915589894783 2202 60-64 0.17776516646944174 2202 65-69 0.2389464675851869 2202 70-74 0.28331884085611847 2202 75-79 0.19118911845146158 2202 80-84 0.1606003927684725 2202 85-89 0.28774313686543707 2202 90-94 0.2731606223024272 2202 95-99 0.24151633552659746 2202 100-104 0.41763485478164597 2202 105-109 0.3528213292396174 2202 110-114 0.3744230035636704 2202 115-119 0.4510829335797837 2202 120-124 0.4282751751498992 2202 125-129 0.43703997812379924 2202 130-134 0.44204512071728885 2202 135-139 0.4684289188491704 2202 140-144 0.5045440139207429 2202 145-149 0.49856311747686277 2202 150-151 0.7958457470208593 1102 1 0.020693823178177695 1102 2 0.0655184031737619 1102 3 0.17990089263980025 1102 4 0.17832057776826815 1102 5 0.1732039885906076 1102 6 0.2065067791948465 1102 7 0.1585388359551132 1102 8 0.1691763298546789 1102 9 0.17295808175306604 1102 10-14 0.16837809002603166 1102 15-19 0.2053630924979828 1102 20-24 0.20403637683258324 1102 25-29 0.15907073210492229 1102 30-34 0.16758140910952335 1102 35-39 0.17452802933829759 1102 40-44 0.17200844919523206 1102 45-49 0.18549888602516518 1102 50-54 0.17709293152309868 1102 55-59 0.1310668409074438 1102 60-64 0.09898624749323659 1102 65-69 0.23479487526840614 1102 70-74 0.20452943079131813 1102 75-79 0.19032882940847884 1102 80-84 0.2147937952996557 1102 85-89 0.2634710934178841 1102 90-94 0.21103254185000964 1102 95-99 0.21700684097083922 1102 100-104 0.37756796303216333 1102 105-109 0.3173853712037271 1102 110-114 0.3057740146634984 1102 115-119 0.38562361955996494 1102 120-124 0.360190067683682 1102 125-129 0.38389420749032155 1102 130-134 0.4646465052301565 1102 135-139 0.4674957515814171 1102 140-144 0.5574143753274967 1102 145-149 0.642823177995588 1102 150-151 0.7372406301742593 2203 1 -0.09095174353948465 2203 2 0.010012184109619682 2203 3 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140-144 0.4452833290887952 2203 145-149 0.46964282322066 2203 150-151 0.8869267271874754 1103 1 -0.009057177901418356 1103 2 0.012916189156925384 1103 3 0.10376855736915047 1103 4 0.12902033220999698 1103 5 0.13982149386314546 1103 6 0.1300385465301872 1103 7 0.10529786744145753 1103 8 0.14980732922301598 1103 9 0.1499775334896043 1103 10-14 0.12260442243813685 1103 15-19 0.12489862529863416 1103 20-24 0.13556845695766384 1103 25-29 0.10166228275280531 1103 30-34 0.06881359214259675 1103 35-39 0.054285988219525905 1103 40-44 0.07209146168823821 1103 45-49 0.10584012276154198 1103 50-54 0.0627243244536757 1103 55-59 0.05788931665231445 1103 60-64 0.012764402820984344 1103 65-69 0.12502269591142579 1103 70-74 0.12690642765542748 1103 75-79 0.03932106187078688 1103 80-84 0.03684698495474947 1103 85-89 0.06901956594597891 1103 90-94 0.10614026523662545 1103 95-99 0.06440594971555669 1103 100-104 0.28854747651515567 1103 105-109 0.22464035501524648 1103 110-114 0.19620424498197053 1103 115-119 0.20590598099054347 1103 120-124 0.2939179097272273 1103 125-129 0.2587003382083992 1103 130-134 0.26737800478463214 1103 135-139 0.3510085366706619 1103 140-144 0.3498376126718199 1103 145-149 0.30390247061348674 1103 150-151 0.5582381107177987 2204 1 -0.12213866598018797 2204 2 0.03592425472082539 2204 3 0.0855566070261986 2204 4 0.1073918809992378 2204 5 0.11993837690440046 2204 6 0.15417103121365727 2204 7 0.1528660174796599 2204 8 0.12396726289218662 2204 9 0.1172980622973796 2204 10-14 0.12191466043321242 2204 15-19 0.12091606034047686 2204 20-24 0.14760308986381432 2204 25-29 0.12203170050516121 2204 30-34 0.1110130486601193 2204 35-39 0.13014316520349212 2204 40-44 0.10472010721269243 2204 45-49 0.14509614654694047 2204 50-54 0.09707385915935873 2204 55-59 0.08966651693950212 2204 60-64 0.0810030897733327 2204 65-69 0.15886057042224166 2204 70-74 0.2056840170533505 2204 75-79 0.13433854186808958 2204 80-84 0.11988147818556882 2204 85-89 0.17212796502836625 2204 90-94 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scores pass #Quality Count 3 110.0 4 679.0 5 761.0 6 691.0 7 793.0 8 535.0 9 411.0 10 367.0 11 336.0 12 318.0 13 375.0 14 467.0 15 1777.0 16 5032.0 17 11658.0 18 22852.0 19 35202.0 20 50730.0 21 71223.0 22 92623.0 23 108190.0 24 125872.0 25 155945.0 26 195540.0 27 211780.0 28 237101.0 29 280574.0 30 337001.0 31 411256.0 32 505827.0 33 633155.0 34 814943.0 35 1111210.0 36 1656093.0 37 2834874.0 38 6470780.0 39 1.1981949E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.936244040651637 10.386843780771414 5.66494388449931 57.01196829407764 2 24.192709121897547 12.507929339053304 41.3199510472262 21.97941049182295 3 23.555328362592263 17.45305634796367 18.60919059146169 40.38242469798238 4 29.73495064703523 23.152315707816687 16.532416553503918 30.580317091644165 5 31.143580109463453 22.32422498578574 21.17552178554431 25.35667311920649 6 25.69850643465779 27.620376868719166 22.611048033718458 24.070068662904585 7 20.455197093450145 17.396936024953973 34.48044927866762 27.667417602928264 8 22.63012417278596 17.500443617752296 27.978032232204864 31.891399977256878 9 24.535039771146398 17.798266148842334 28.828403120270778 28.838290959740487 10-14 28.473376551584455 21.870477394018447 22.272100703194177 27.384045351202918 15-19 28.55923025236709 22.316670322801773 21.83195951838848 27.292139906442657 20-24 28.65340130650998 21.750662565164617 21.58671477832366 28.009221350001745 25-29 29.22454666527699 21.52788353294538 21.533638823477872 27.713930978299757 30-34 28.588458960306397 21.749886208442017 21.560146520580485 28.101508310671104 35-39 27.617681343986277 21.268208840062666 22.208395945863955 28.905713870087098 40-44 28.93565661555603 20.378638343898807 21.795994150602695 28.889710889942467 45-49 29.143516910908833 20.26818399816146 21.594954672601535 28.993344418328164 50-54 28.56649729181551 22.048142246291995 20.858773532100827 28.52658692979167 55-59 28.866212620400848 20.513552424895014 21.50405594820545 29.116179006498683 60-64 28.55563344579795 20.46122828993293 21.400503208462553 29.582635055806566 65-69 28.586082453388556 21.19126551606018 21.727873123199696 28.49477890735157 70-74 29.106389572007075 21.26765263944185 21.078475209387413 28.547482579163656 75-79 29.216926070930917 21.649384766049735 21.332708355620124 27.800980807399224 80-84 29.79964626468065 20.359019025114318 22.07873229432209 27.76260241588294 85-89 28.671760672554413 21.86433029067367 21.603227099485565 27.86068193728636 90-94 28.953376225914802 22.105645242769086 21.26985245382206 27.67112607749406 95-99 28.97420537474395 21.83482173729346 20.819564504971222 28.37140838299137 100-104 28.959216242424006 22.574001525320217 20.834253809303632 27.63252842295214 105-109 29.313020085551024 22.542492723266236 21.03247491203125 27.112012279151486 110-114 30.484650263299816 22.55202552933945 20.777320634104505 26.18600357325623 115-119 29.29777438308072 23.511617451237505 19.80955740101467 27.38105076466711 120-124 30.01798667034735 23.62870262850434 19.606167347160707 26.747143353987607 125-129 30.477944934559204 24.216840618724884 18.969735254685947 26.335479192029965 130-134 31.09439536073057 24.149819707517146 19.404884266109576 25.350900665642712 135-139 30.52990436165746 24.832878430896514 19.96522434387027 24.671992863575756 140-144 31.905814842095953 25.551106556534975 19.870230822272802 22.67284777909627 145-149 32.09025057537271 26.29244278211129 19.65644169564421 21.960864946871787 150-151 32.10739712038319 27.17340302762211 18.230100718621 22.4890991333737 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 971.0 1 787.5 2 573.5 3 554.5 4 557.0 5 538.5 6 543.0 7 547.0 8 570.5 9 636.0 10 716.5 11 759.0 12 796.5 13 827.5 14 905.5 15 963.0 16 970.0 17 994.5 18 973.5 19 982.5 20 1009.5 21 1081.5 22 1277.5 23 1589.0 24 2152.5 25 2519.0 26 2800.5 27 3125.0 28 3839.0 29 4884.5 30 6131.0 31 6456.0 32 5726.5 33 6621.0 34 7656.0 35 9497.0 36 15863.5 37 25938.0 38 31371.0 39 36241.0 40 57409.5 41 81336.0 42 137776.0 43 244271.0 44 468830.5 45 749351.5 46 1104506.5 47 1328779.5 48 1313925.0 49 1174648.5 50 983111.0 51 1050319.0 52 1161148.0 53 1181262.5 54 1246341.0 55 1301477.5 56 1275842.5 57 1105519.5 58 1086379.0 59 1071300.5 60 1032384.0 61 981835.5 62 739638.5 63 675502.0 64 589750.5 65 467240.0 66 448125.0 67 430328.0 68 417862.5 69 397806.0 70 361906.5 71 331519.5 72 273023.0 73 245731.5 74 290832.0 75 229234.5 76 168831.5 77 198741.0 78 191836.5 79 180001.0 80 195233.5 81 157577.0 82 247432.5 83 246570.5 84 127121.5 85 126327.5 86 104529.0 87 81130.0 88 68697.5 89 36541.5 90 4113.0 91 2372.0 92 2257.0 93 1864.5 94 499.5 95 208.5 96 133.0 97 93.5 98 57.0 99 37.0 100 64.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0020303831325921263 2 0.004515487487587696 3 0.005509529229585925 4 0.004695260994119291 5 0.00489618432494872 6 0.0 7 0.0 8 4.2299648595669295E-5 9 0.002999750079576214 10-14 0.0024900393139983988 15-19 0.004184140240254954 20-24 0.011603498603935348 25-29 0.019605182129949453 30-34 0.02806934181394288 35-39 0.034628607322844666 40-44 0.04876867485423365 45-49 0.050418361149464755 50-54 0.058138752012317656 55-59 0.057026271254251554 60-64 0.05951419558582017 65-69 0.056869762554447575 70-74 0.05366697416161215 75-79 0.05345829589520685 80-84 0.04910636704885574 85-89 0.046228580956063706 90-94 0.04281076934953363 95-99 0.04202117590908114 100-104 0.03386721364812262 105-109 0.02997494098317778 110-114 0.026648073621128393 115-119 0.020578779041793113 120-124 0.019282294812335846 125-129 0.016639976763393037 130-134 0.016087966349219555 135-139 0.01374809078773578 140-144 0.015148914150395695 145-149 0.011736037502868444 150-151 0.008734877435005709 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 2.836903E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 2.847178555873493 #Duplication Level Percentage of deduplicated Percentage of total 1 71.99899763707836 2.049940021166758 2 10.016119728618609 0.5703536260876867 3 4.595711322966505 0.39254432183205706 4 1.8841261725798963 0.2145777454051793 5 1.7640366498868223 0.25112636606663385 6 1.280914713785143 0.21881957429951354 7 0.976366028621937 0.19459218935630207 8 0.6766355354662908 0.15412017493772814 9 0.5383804754887569 0.13795788102413073 >10 3.775691392890058 2.166395219479044 >50 0.6918664385585691 1.4028047694194734 >100 0.9956305381337068 6.654044718359477 >500 0.27193528005809986 5.500929939308862 >1k 0.39652194438262517 25.165435496571032 >5k 0.07758981304988966 15.350031044960048 >10k+ 0.0594763284349204 39.57632691172594 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT 157104 0.5537869994145024 No Hit CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG 157060 0.553631900702985 No Hit CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGG 137639 0.4851734444216105 No Hit CTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCG 119406 0.42090265335120725 No Hit CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC 118030 0.41605229364557056 No Hit CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC 101164 0.3566001375443573 No Hit CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG 95395 0.33626458148198934 No Hit CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT 94486 0.3330603831008674 No Hit CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT 92507 0.32608446605329827 No Hit CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC 84989 0.29958373620811146 No Hit CCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC 80825 0.2849057581454142 No Hit CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG 80736 0.28459203575166303 No Hit CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT 79617 0.2806475935201168 No Hit CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT 77471 0.27308300636292465 No Hit GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA 75622 0.2665653355084752 No Hit CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC 71798 0.253085847489322 No Hit CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA 65952 0.23247886868179843 No Hit CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA 62970 0.2219674060057746 No Hit CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT 61083 0.2153157862641056 No Hit CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT 59858 0.21099769713663102 No Hit GTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG 58381 0.2057913153886474 No Hit GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC 58229 0.20525551983976895 No Hit GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG 58075 0.20471267434945783 No Hit CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT 57201 0.20163184994340658 No Hit CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA 56654 0.19970369096158733 No Hit CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC 55932 0.19715866210441457 No Hit CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA 55666 0.19622101989387725 No Hit CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT 55362 0.1951494287961203 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 55131 0.19433516056065364 Illumina Single End PCR Primer 1 (100% over 50bp) CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC 54957 0.19372181565601646 No Hit CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG 54219 0.19112038726738279 No Hit CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG 53453 0.18842025969869255 No Hit CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG 53239 0.18766591596540314 No Hit CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC 52987 0.18677762334489406 No Hit GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA 52758 0.18597040505086004 No Hit ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG 52333 0.1844722924964301 No Hit CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC 52196 0.1839893715082962 No Hit AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC 51730 0.1823467351544977 No Hit GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT 51728 0.1823396852130651 No Hit CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG 51212 0.1805208003234513 No Hit CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC 48913 0.17241689264666432 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG 48347 0.17042175922123529 No Hit CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC 47810 0.16852884994657907 No Hit CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA 46636 0.16439053432563608 No Hit CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG 46368 0.16344584217366614 No Hit CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGTGGGTC 46131 0.16261042411390167 No Hit CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA 45129 0.15907840345616328 No Hit CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC 43628 0.15378742241098833 No Hit CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGG 43300 0.15263123201604004 No Hit GTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCG 43092 0.15189803810704844 No Hit CTTCCGTACGCCACATTTCCCGCGCCGCGCCGCGCGGCGGGGATTCGGCG 42767 0.15075242262424907 No Hit CTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGT 42467 0.14969493140935733 No Hit CTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCA 41822 0.1474213252973401 No Hit CATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCC 41699 0.14698775389923446 No Hit CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGG 40950 0.14434755083272147 No Hit CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA 40203 0.14171439770764105 No Hit CTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT 39752 0.14012463591458713 No Hit CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC 39130 0.13793210412904494 No Hit AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA 38947 0.13728703448796098 No Hit CTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGGGAGAGC 38933 0.13723768489793273 No Hit CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCGTCTC 38743 0.1365679404618346 No Hit CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG 38656 0.13626126800951602 No Hit CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT 38253 0.13484070481084479 No Hit GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT 37434 0.13195375379419033 No Hit CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG 37242 0.13127695941665965 No Hit AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG 37080 0.1307059141606181 No Hit CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG 36974 0.1303322672646897 No Hit CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCG 35945 0.12670507239761106 No Hit CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG 35688 0.12579915492352048 No Hit CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC 35430 0.12488971247871358 No Hit GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC 35249 0.12425169277906224 No Hit CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC 34864 0.1228945790532845 No Hit CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT 34154 0.12039184984470742 No Hit CGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT 33934 0.11961635628712014 No Hit CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG 33887 0.11945068266345377 No Hit CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA 33837 0.1192744341276385 No Hit AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC 33618 0.11850246554076752 No Hit GTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGC 33528 0.1181852181763 No Hit AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG 33385 0.11768114736386828 No Hit CTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGC 32395 0.11419142635472555 No Hit CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT 31784 0.11203766924706272 No Hit CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC 31712 0.11178387135548871 No Hit CGGGTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGA 31694 0.1117204218825952 No Hit CTCCCGTCCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT 31400 0.11068408049200132 No Hit GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT 31302 0.11033863336180334 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 31290 0.11029633371320768 No Hit CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT 31244 0.1101341850602576 No Hit CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA 31047 0.10943976582914537 No Hit CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT 30929 0.10902381928462128 No Hit GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA 30621 0.10793812830399911 No Hit CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA 30342 0.1069546614741498 No Hit GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTA 29949 0.10556934798264164 No Hit CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATG 29835 0.10516750132098278 No Hit GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC 29517 0.10404656063319755 No Hit AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG 29404 0.10364823894225497 No Hit CCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG 29147 0.1027423214681644 No Hit GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT 28974 0.1021325015342435 No Hit CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT 28964 0.10209725182708045 No Hit GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA 28843 0.10167073037040744 No Hit CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT 28760 0.10137815780095406 No Hit GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC 28743 0.10131823329877687 No Hit CTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT 28570 0.10070841336485598 No Hit CTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGG 28439 0.10024664220101992 No Hit CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG 28415 0.10016204290382857 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.534204024600066E-4 0.0 0.0 2 5.851451389067585E-4 0.0 0.0 3 7.014691725448491E-4 0.0 0.0 4 7.719685868709645E-4 0.0 0.0 5 8.177932061829397E-4 0.0 0.0 6 0.001061016185608038 0.0 0.0 7 0.0011033158342037073 0.0 0.0 8 0.0011456154827993767 0.0 0.0 9 0.001216114897125492 0.0 0.0 10-11 0.0013712136086429462 0.0 0.0 12-13 0.0016109116173517388 0.0 0.0 14-15 0.0020339081033084318 0.0 0.0 16-17 0.002636678095796719 0.0 0.0 18-19 0.003375159460862779 0.0 0.0 20-21 0.00430398924460935 0.0 0.0 22-23 0.006314985038261794 0.0 0.0 24-25 0.009480408741504381 0.0 0.0 26-27 0.014147469969893224 0.0 0.0 28-29 0.022138578583758416 0.0 0.0 30-31 0.03322461148654007 0.0 0.0 32-33 0.047532467624025215 0.0 0.0 34-35 0.06445408954765108 0.0 0.0 36-37 0.08409170140819056 0.0 0.0 38-39 0.10749574447910273 0.0 0.0 40-41 0.1361678562855339 0.0 0.0 42-43 0.17014857399072159 0.0 0.0 44-45 0.20408875453267172 3.5249707163057744E-6 0.0 46-47 0.24179712877035275 3.5249707163057744E-6 0.0 48-49 0.2967144100450385 3.5249707163057744E-6 0.0 50-51 0.34556697920232027 3.5249707163057744E-6 0.0 52-53 0.3969698646728492 3.5249707163057744E-6 0.0 54-55 0.4561699853678466 3.5249707163057744E-6 0.0 56-57 0.5212991068076702 3.5249707163057744E-6 0.0 58-59 0.5896712013064951 3.5249707163057744E-6 0.0 60-61 0.6632179528168569 7.049941432611549E-6 0.0 62-63 0.7411603428104521 7.049941432611549E-6 0.0 64-65 0.8351554494461038 7.049941432611549E-6 0.0 66-67 0.9324287788479197 7.049941432611549E-6 0.0 68-69 1.0420677055225362 8.812426790764435E-6 0.0 70-71 1.1676888494248834 1.0574912148917322E-5 0.0 72-73 1.3049617840299792 1.0574912148917322E-5 0.0 74-75 1.445255618538949 1.0574912148917322E-5 0.0 76-77 1.6034034297260076 1.0574912148917322E-5 0.0 78-79 1.7735731535410268 1.0574912148917322E-5 0.0 80-81 1.95985551850028 1.0574912148917322E-5 0.0 82-83 2.1663148158396677 1.0574912148917322E-5 0.0 84-85 2.40064076917681 1.0574912148917322E-5 0.0 86-87 2.6562927953476025 1.0574912148917322E-5 0.0 88-89 2.9267408861000885 1.0574912148917322E-5 0.0 90-91 3.2319117008935447 1.0574912148917322E-5 0.0 92-93 3.5935772213572337 1.0574912148917322E-5 0.0 94-95 3.9865673940913737 1.0574912148917322E-5 0.0 96-97 4.416185537538647 1.0574912148917322E-5 0.0 98-99 4.904268845286568 1.0574912148917322E-5 0.0 100-101 5.457585261110443 1.0574912148917322E-5 0.0 102-103 6.028621704725188 1.0574912148917322E-5 0.0 104-105 6.6580087510923 1.0574912148917322E-5 0.0 106-107 7.359354549662079 1.0574912148917322E-5 0.0 108-109 8.083596795519622 1.0574912148917322E-5 0.0 110-111 8.909411072567515 1.0574912148917322E-5 0.0 112-113 9.788136922552516 1.0574912148917322E-5 0.0 114-115 10.676776752677128 1.0574912148917322E-5 0.0 116-117 11.647081341871752 1.0574912148917322E-5 0.0 118-119 12.799196870671997 1.0574912148917322E-5 1.7624853581528872E-6 120-121 14.043248923209571 1.0574912148917322E-5 3.5249707163057744E-6 122-123 15.355569083609838 1.0574912148917322E-5 3.5249707163057744E-6 124-125 16.767030807891565 1.0574912148917322E-5 3.5249707163057744E-6 126-127 18.382093078261754 1.4099882865223098E-5 3.5249707163057744E-6 128-129 20.118957186763172 1.4099882865223098E-5 3.5249707163057744E-6 130-131 21.74675870130209 1.4099882865223098E-5 3.5249707163057744E-6 132-133 23.458484481140175 1.4099882865223098E-5 3.5249707163057744E-6 134-135 25.163103920014187 1.4099882865223098E-5 3.5249707163057744E-6 136-137 26.959340872775698 1.4099882865223098E-5 3.5249707163057744E-6 138-139 28.772020051443423 1.4099882865223098E-5 3.5249707163057744E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAACCC 3450 0.0 111.135506 145 CATCTCC 2835 0.0 103.5424 145 CCTGGCC 12980 0.0 97.10027 5 TCCGCTT 5555 0.0 91.192924 2 TGGCCCG 13920 0.0 90.7475 7 TGCTCCC 2410 0.0 88.4192 145 ACTTATG 8240 0.0 85.66594 6 CTTATGC 8405 0.0 84.5878 7 CCCTCGT 1370 0.0 84.1097 1 GCCAGTA 3315 0.0 81.11044 3 GTAGGGG 7070 0.0 80.06595 145 CGGTGGG 4025 0.0 79.77266 145 TCCACTC 10420 0.0 79.287575 2 TTATGCT 9085 0.0 78.735435 8 CCTCAAC 3610 0.0 78.69523 1 AGACGTG 4875 0.0 78.646 4 CCAGACG 8995 0.0 78.47553 6 CCAGTAG 3470 0.0 77.48899 4 CTCGATC 9065 0.0 76.828995 1 CAGTAGC 3495 0.0 76.72733 5 >>END_MODULE