##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2V3NBCXX l01n02 library 3 mfa.3420000000455d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22468735 Sequences flagged as poor quality 0 Sequence length 151 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.68779524080906 38.0 38.0 38.0 32.0 38.0 2 36.616433145880265 38.0 38.0 38.0 32.0 38.0 3 36.65604427663596 38.0 38.0 38.0 32.0 38.0 4 36.62160272930363 38.0 38.0 38.0 32.0 38.0 5 36.45702007700923 38.0 38.0 38.0 32.0 38.0 6 38.737639257394775 40.0 38.0 40.0 38.0 40.0 7 38.80024331587871 40.0 38.0 40.0 38.0 40.0 8 38.84225654003218 40.0 40.0 40.0 38.0 40.0 9 38.830893016451526 40.0 40.0 40.0 38.0 40.0 10-14 38.81753987485277 40.0 38.8 40.0 38.0 40.0 15-19 38.7629943563801 40.0 38.0 40.0 38.0 40.0 20-24 38.724087341810744 40.0 38.0 40.0 38.0 40.0 25-29 38.70103500708874 40.0 38.0 40.0 38.0 40.0 30-34 38.63741940968195 40.0 38.0 40.0 38.0 40.0 35-39 38.57218570604887 40.0 38.0 40.0 38.0 40.0 40-44 38.43114941717903 40.0 38.0 40.0 38.0 40.0 45-49 38.28728551028796 40.0 38.0 40.0 38.0 40.0 50-54 38.21358528639908 40.0 38.0 40.0 38.0 40.0 55-59 38.148123283309005 40.0 38.0 40.0 38.0 40.0 60-64 38.10684978927385 40.0 38.0 40.0 38.0 40.0 65-69 37.884426212690656 40.0 38.0 40.0 35.6 40.0 70-74 37.740595872442306 40.0 38.0 40.0 32.0 40.0 75-79 37.63233228750973 40.0 38.0 40.0 32.0 40.0 80-84 37.50813986635207 40.0 38.0 40.0 32.0 40.0 85-89 37.362132394191306 40.0 38.0 40.0 32.0 40.0 90-94 37.186440616260775 38.0 38.0 40.0 32.0 40.0 95-99 37.01508811243713 38.0 38.0 40.0 32.0 40.0 100-104 35.61379467068351 38.0 35.8 38.8 29.0 39.2 105-109 37.16899233534954 38.8 38.0 40.0 32.0 40.0 110-114 37.08617787338718 38.4 38.0 40.0 32.0 40.0 115-119 36.881313834534964 38.0 38.0 40.0 32.0 40.0 120-124 36.4819047267236 38.0 38.0 40.0 29.0 40.0 125-129 36.14117519299596 38.0 38.0 40.0 27.0 40.0 130-134 35.7065670230211 38.0 38.0 40.0 24.0 40.0 135-139 35.214285948897434 38.0 38.0 40.0 22.0 40.0 140-144 34.705298148738684 38.0 33.2 40.0 20.2 40.0 145-149 34.03718441647917 38.0 32.0 40.0 13.0 40.0 150-151 29.04373697495653 30.0 22.5 38.0 13.0 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.11400658678800824 2201 2 0.19479423503639026 2201 3 0.21616483810787912 2201 4 0.2571127992611082 2201 5 0.28217533062407085 2201 6 0.26517639695707373 2201 7 0.2096511254744584 2201 8 0.21287983918357867 2201 9 0.1821348627731183 2201 10-14 0.21596403456945268 2201 15-19 0.23113022818893825 2201 20-24 0.20991001692719635 2201 25-29 0.22102345455708416 2201 30-34 0.19376498899070782 2201 35-39 0.2127995482790226 2201 40-44 0.20386654189313447 2201 45-49 0.1933461835926238 2201 50-54 0.18574681864291165 2201 55-59 0.17574139827522828 2201 60-64 0.16816800582697056 2201 65-69 0.24133122055842904 2201 70-74 0.26032760364424945 2201 75-79 0.15938780161438615 2201 80-84 0.1607831632075758 2201 85-89 0.19215252422534945 2201 90-94 0.2531282316179002 2201 95-99 0.19247887763090432 2201 100-104 0.24559606103029097 2201 105-109 0.2954733582906002 2201 110-114 0.30947025830487007 2201 115-119 0.3178268978674481 2201 120-124 0.3309272094298663 2201 125-129 0.35362875569198593 2201 130-134 0.4181042134813282 2201 135-139 0.42783514275639334 2201 140-144 0.4824339516904672 2201 145-149 0.3952582955735622 2201 150-151 0.5906734059640151 1101 1 0.08457309674275137 1101 2 0.21682941675917533 1101 3 0.1414685833446967 1101 4 0.2391015894271149 1101 5 0.2111430254564084 1101 6 0.21334343037060677 1101 7 0.140597708585517 1101 8 0.17887981530795827 1101 9 0.14699753613611932 1101 10-14 0.1490290331571984 1101 15-19 0.18809904989535653 1101 20-24 0.19896096629990012 1101 25-29 0.16776880433948804 1101 30-34 0.12470846078157649 1101 35-39 0.16348757230613842 1101 40-44 0.18028562971038298 1101 45-49 0.20424629186812382 1101 50-54 0.15154609400624963 1101 55-59 0.1530569425334818 1101 60-64 0.15469524461857986 1101 65-69 0.22739742241822825 1101 70-74 0.24736257997335542 1101 75-79 0.184742008963255 1101 80-84 0.1395578395139907 1101 85-89 0.18495286483247497 1101 90-94 0.19732004735264752 1101 95-99 0.24329708049118892 1101 100-104 0.38517948792206624 1101 105-109 0.34323117265766 1101 110-114 0.33429107435056693 1101 115-119 0.3863488720172157 1101 120-124 0.4334663310180602 1101 125-129 0.454151066130251 1101 130-134 0.4536415340118012 1101 135-139 0.4870368022849405 1101 140-144 0.4420630560866101 1101 145-149 0.4962333503002725 1101 150-151 0.552617887584379 2202 1 0.039965425024441004 2202 2 0.15780719822625144 2202 3 0.17921965545823326 2202 4 0.248939280563512 2202 5 0.2151372369431357 2202 6 0.20061233109720433 2202 7 0.21356918105471578 2202 8 0.20704815079416505 2202 9 0.19747466306704098 2202 10-14 0.19349448316695117 2202 15-19 0.20830757467265215 2202 20-24 0.2211090086632197 2202 25-29 0.20359803266033794 2202 30-34 0.19523257817449036 2202 35-39 0.21454584659625198 2202 40-44 0.2212137895153603 2202 45-49 0.19423354308468532 2202 50-54 0.19163516102914002 2202 55-59 0.18954563106558453 2202 60-64 0.20853824144779054 2202 65-69 0.26696167896449907 2202 70-74 0.2850178618028707 2202 75-79 0.21436517423943258 2202 80-84 0.19416233421952 2202 85-89 0.29279644293791307 2202 90-94 0.3286451141189488 2202 95-99 0.28211007534420673 2202 100-104 0.43722387062882007 2202 105-109 0.4010249328454307 2202 110-114 0.362538139745169 2202 115-119 0.46393149696402247 2202 120-124 0.4202052107089216 2202 125-129 0.45571454300760195 2202 130-134 0.4328553993424151 2202 135-139 0.4677495851893383 2202 140-144 0.47798297225808284 2202 145-149 0.47344704523236913 2202 150-151 0.8306822986012392 1102 1 -0.008755313416152433 1102 2 0.091436500214229 1102 3 0.15374687049482816 1102 4 0.15178681017182072 1102 5 0.06432411563599771 1102 6 0.1585109541567391 1102 7 0.12243469700408838 1102 8 0.16297538182577398 1102 9 0.18973669407465366 1102 10-14 0.15784502821292534 1102 15-19 0.1766859851071203 1102 20-24 0.1758466002532728 1102 25-29 0.1355708842154968 1102 30-34 0.1382944503038459 1102 35-39 0.1433640409354311 1102 40-44 0.14292715125826305 1102 45-49 0.13727882701054028 1102 50-54 0.15239206764871938 1102 55-59 0.07942021495338736 1102 60-64 0.05176469270573847 1102 65-69 0.17818970735230266 1102 70-74 0.1890413907338626 1102 75-79 0.1648514233643752 1102 80-84 0.1873535628496228 1102 85-89 0.17376863972067724 1102 90-94 0.17190275961214496 1102 95-99 0.1789719174141453 1102 100-104 0.32089208370006617 1102 105-109 0.2892814837693223 1102 110-114 0.28409027788151064 1102 115-119 0.3558801972463428 1102 120-124 0.3218914931921617 1102 125-129 0.33685496149451666 1102 130-134 0.44584816495345336 1102 135-139 0.40100514223599504 1102 140-144 0.47571055829615716 1102 145-149 0.5388230468632642 1102 150-151 0.651527217483892 2203 1 -0.0220406134189588 2203 2 0.07533235160668994 2203 3 0.08925909453293457 2203 4 0.12719742489489505 2203 5 0.08629384461898582 2203 6 0.2236796114241315 2203 7 0.16068172082007948 2203 8 0.1686667423951036 2203 9 0.1533717276678317 2203 10-14 0.14776286422540608 2203 15-19 0.15688222938111096 2203 20-24 0.18532234163075145 2203 25-29 0.15684087762257093 2203 30-34 0.14793547373260907 2203 35-39 0.14509774282037569 2203 40-44 0.12661364502007189 2203 45-49 0.13511914673853198 2203 50-54 0.08672528384813205 2203 55-59 0.06714954544518292 2203 60-64 0.06586351451171168 2203 65-69 0.1709129138305272 2203 70-74 0.16767171497148325 2203 75-79 0.12314343804602856 2203 80-84 0.16712239154902875 2203 85-89 0.1556679512165573 2203 90-94 0.20500785087443063 2203 95-99 0.21644690117280874 2203 100-104 0.44916970436872106 2203 105-109 0.270661459245062 2203 110-114 0.35610567106895274 2203 115-119 0.4162921806867885 2203 120-124 0.40723526870378635 2203 125-129 0.4323311202754496 2203 130-134 0.4289383493133059 2203 135-139 0.4793063379912965 2203 140-144 0.5556972431533538 2203 145-149 0.5641875953768505 2203 150-151 1.011651990085113 1103 1 -0.035624059535209085 1103 2 0.08707661342912587 1103 3 0.10147220410817681 1103 4 0.10820443733178564 1103 5 0.05599389895015605 1103 6 0.11479226570637735 1103 7 0.10766219897671192 1103 8 0.13218809979059643 1103 9 0.15413706692837081 1103 10-14 0.10445416210048108 1103 15-19 0.10471731989057531 1103 20-24 0.13572439618605614 1103 25-29 0.07660367061240692 1103 30-34 0.064071088509003 1103 35-39 0.07074557374036061 1103 40-44 0.05998365555682739 1103 45-49 0.09715340615380796 1103 50-54 0.03886712246052326 1103 55-59 0.045761127018352 1103 60-64 -7.833273235817728E-4 1103 65-69 0.09475470230470506 1103 70-74 0.14633569361770782 1103 75-79 0.09205146731842007 1103 80-84 0.0556448604054296 1103 85-89 0.09797579249968891 1103 90-94 0.12144292750897279 1103 95-99 0.0734397006373726 1103 100-104 0.2941758648309687 1103 105-109 0.24551151909925295 1103 110-114 0.18663044310204668 1103 115-119 0.22655924122349091 1103 120-124 0.3324727278764499 1103 125-129 0.289159046024821 1103 130-134 0.3018873461844933 1103 135-139 0.3837501520535014 1103 140-144 0.40749917670710545 1103 145-149 0.3335572901089776 1103 150-151 0.5198122939891725 2204 1 -0.10190011120137399 2204 2 0.07522415872024624 2204 3 0.08516111789899838 2204 4 0.1283220669340821 2204 5 0.06775802887736404 2204 6 0.15720287889831042 2204 7 0.1298201287914864 2204 8 0.12473556730608948 2204 9 0.1363704647263404 2204 10-14 0.12301071401949315 2204 15-19 0.13676343397634128 2204 20-24 0.16571902864912857 2204 25-29 0.12751716534704371 2204 30-34 0.09339743886625484 2204 35-39 0.10913681862715663 2204 40-44 0.083348722698382 2204 45-49 0.1109125417925867 2204 50-54 0.07555234142667189 2204 55-59 0.06772453866828698 2204 60-64 0.08392085862647747 2204 65-69 0.16547466299120828 2204 70-74 0.19369344436610447 2204 75-79 0.1054283749961229 2204 80-84 0.09742531296996049 2204 85-89 0.1392664913307584 2204 90-94 0.1684308978969824 2204 95-99 0.10200408313396991 2204 100-104 0.3484638116640255 2204 105-109 0.284144756160039 2204 110-114 0.29089242629778767 2204 115-119 0.33908713254747624 2204 120-124 0.3578602614113535 2204 125-129 0.3240540466587305 2204 130-134 0.37536432139665266 2204 135-139 0.37351570647587806 2204 140-144 0.46412440965992374 2204 145-149 0.4381497265848253 2204 150-151 0.5318527516199261 2205 1 -0.05297650292689582 2205 2 0.01133274926048955 2205 3 0.027420511806738546 2205 4 0.04697645385746796 2205 5 0.041068448947562786 2205 6 0.08560431592768225 2205 7 -0.005842190106307044 2205 8 0.0417552694005181 2205 9 0.05671579383470515 2205 10-14 0.041098948171104155 2205 15-19 0.06225880896293745 2205 20-24 0.07672340860631977 2205 25-29 0.05567470696790622 2205 30-34 0.03826936578579421 2205 35-39 0.04157434706963414 2205 40-44 0.04866065940466768 2205 45-49 0.04810080624987023 2205 50-54 -0.003941215219938954 2205 55-59 -0.009411576773224795 2205 60-64 0.011346013043869618 2205 65-69 0.08854018056521795 2205 70-74 0.12409626644447513 2205 75-79 0.015897306726216698 2205 80-84 -0.02615768245163963 2205 85-89 0.039864500368409495 2205 90-94 0.06935747042589213 2205 95-99 0.012574001135746471 2205 100-104 0.3415098437088986 2205 105-109 0.1849819660323604 2205 110-114 0.23122926603398497 2205 115-119 0.1975911255364977 2205 120-124 0.23969631950367187 2205 125-129 0.24898814042960993 2205 130-134 0.27337098663280557 2205 135-139 0.21901603792722568 2205 140-144 0.29649947086249284 2205 145-149 0.2290893963865699 2205 150-151 0.3699529341035728 1216 1 -0.00723940910545906 1216 2 0.012031352407582574 1216 3 0.0018737583345185271 1216 4 0.06685123313314278 1216 5 -0.014888633085796243 1216 6 0.06476383376499228 1216 7 -0.04839576558689629 1216 8 0.014045849912598385 1216 9 0.013177461204172403 1216 10-14 -0.04325274974203097 1216 15-19 -0.022036965899353333 1216 20-24 -0.008552144708687592 1216 25-29 -0.009084054435518851 1216 30-34 -0.02017766802449472 1216 35-39 -0.022323438421501862 1216 40-44 -0.07149174658279378 1216 45-49 -0.05962341256365988 1216 50-54 -0.11065846133939061 1216 55-59 -0.0976284366061222 1216 60-64 -0.0742571924183082 1216 65-69 -0.03665018722497848 1216 70-74 0.03956216503659249 1216 75-79 -0.08642652995099809 1216 80-84 -0.045210315636857956 1216 85-89 -0.05366974632474353 1216 90-94 -0.015034601665469438 1216 95-99 -0.05169967287419297 1216 100-104 0.0972346021206576 1216 105-109 0.07786508786362845 1216 110-114 0.06010687748143795 1216 115-119 0.10900769715884451 1216 120-124 0.12598359148486793 1216 125-129 0.05573183198599452 1216 130-134 0.094712227077693 1216 135-139 0.05245534371122318 1216 140-144 0.08971643509259764 1216 145-149 0.0439220946756933 1216 150-151 0.5010511821428665 2206 1 -0.363176574067424 2206 2 -0.2071746256103637 2206 3 -0.13220724594445699 2206 4 -0.13287510764913435 2206 5 -0.05624755052019026 2206 6 -0.029529336857578414 2206 7 -0.12378956132672414 2206 8 -0.12169473174651557 2206 9 -0.1043922892217779 2206 10-14 -0.1013352971952628 2206 15-19 -0.11868740869739725 2206 20-24 -0.050514695565631484 2206 25-29 -0.11001084965747054 2206 30-34 -0.1285461582734797 2206 35-39 -0.12175410777300755 2206 40-44 -0.14393068299628453 2206 45-49 -0.179088628761356 2206 50-54 -0.24806797076838905 2206 55-59 -0.271818986206668 2206 60-64 -0.2828947053459743 2206 65-69 -0.21522683375247453 2206 70-74 -0.13457303688721112 2206 75-79 -0.28433570838626565 2206 80-84 -0.29815927433673295 2206 85-89 -0.2813387381718684 2206 90-94 -0.3157707486272159 2206 95-99 -0.34387153436440343 2206 100-104 -0.012136857129370071 2206 105-109 -0.09049557628255656 2206 110-114 -0.1457508292022709 2206 115-119 -0.16528205445887068 2206 120-124 -0.14667497691972642 2206 125-129 -0.14715198622509718 2206 130-134 -0.21953939841271364 2206 135-139 -0.29985908815371687 2206 140-144 -0.29108982923458626 2206 145-149 -0.4081453358679994 2206 150-151 -0.23851315302428944 2207 1 -0.21296026190196926 2207 2 -0.2818134514988202 2207 3 -0.23095717921614067 2207 4 -0.2030606009109306 2207 5 -0.2950553439171557 2207 6 -0.2548747709630561 2207 7 -0.2702874123958594 2207 8 -0.2633196953211865 2207 9 -0.3110290155348494 2207 10-14 -0.3249342729300295 2207 15-19 -0.398676020451461 2207 20-24 -0.4359611113289361 2207 25-29 -0.49050280532979684 2207 30-34 -0.5521909709688373 2207 35-39 -0.5625256140919035 2207 40-44 -0.5660493585644488 2207 45-49 -0.5440894894151285 2207 50-54 -0.6430368949438261 2207 55-59 -0.6901740689862237 2207 60-64 -0.8088100179245501 2207 65-69 -0.7751734188643837 2207 70-74 -0.7037797994481494 2207 75-79 -0.998186321348598 2207 80-84 -0.8850768320932971 2207 85-89 -0.8977742909295969 2207 90-94 -0.9151901716238484 2207 95-99 -0.9519298229208459 2207 100-104 -0.7879223109309521 2207 105-109 -0.7082755768982878 2207 110-114 -0.7468554079903811 2207 115-119 -0.7838697197713955 2207 120-124 -0.9064085785762757 2207 125-129 -0.9702769247926355 2207 130-134 -0.965999665095282 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scores pass #Quality Count 3 104.0 4 580.0 5 590.0 6 570.0 7 611.0 8 444.0 9 318.0 10 273.0 11 235.0 12 258.0 13 314.0 14 451.0 15 2331.0 16 6642.0 17 11535.0 18 18824.0 19 27416.0 20 39298.0 21 54746.0 22 72480.0 23 87854.0 24 106568.0 25 143730.0 26 199195.0 27 177304.0 28 186570.0 29 221392.0 30 270607.0 31 334833.0 32 418432.0 33 528251.0 34 685888.0 35 931660.0 36 1369514.0 37 2302747.0 38 5175708.0 39 9090462.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.145674881536543 10.85480753101222 5.694786895736446 53.304730691714795 2 24.12066756303051 13.357211345600156 38.546047053716094 23.976074037653245 3 23.553428083843173 16.51911470962186 19.350364877085486 40.577092329449485 4 29.317373518672063 21.982816001753267 16.863147434838083 31.836663044736586 5 31.254776016323145 21.73438875514399 21.131831247222962 25.879003981309907 6 26.161900080267092 26.862224330831264 23.250828317660073 23.72504727124157 7 20.5204565366052 18.53417203950289 34.09106476176785 26.854306662124056 8 22.667260439974985 18.38038746467758 27.968017954907292 30.984334140440144 9 24.478252173153702 18.410610663912887 28.424315465373525 28.686821697559882 10-14 28.371120292863417 22.103422788123016 22.460795507266575 27.064661411746993 15-19 28.563837876375747 22.522038448951523 21.912297375646293 27.001826299026437 20-24 28.579965494866215 22.225891552137327 21.60539239327552 27.588750559720935 25-29 29.131797303883967 22.03005905343092 21.613405077270112 27.224738565415002 30-34 28.466650255274082 21.855132171543502 21.90052705215774 27.777690521024674 35-39 27.366653371362286 21.70869581469671 22.438809974744384 28.48584083919662 40-44 29.013209477099334 20.557221073027684 22.088055242865025 28.34151420700796 45-49 28.651647694243305 20.621970429157706 21.772857690237977 28.953524186361008 50-54 28.23586243782402 22.308138132753133 21.17538608677037 28.280613342652476 55-59 28.462103151674896 21.152225748352734 21.910807972246364 28.474863127726007 60-64 28.18255303792298 21.34499960103497 21.580606731624414 28.89184062941763 65-69 28.094117446302064 21.904693572820026 21.92826300349644 28.07292597738147 70-74 28.880393423568307 21.855977279424145 21.398191832000148 27.86543746500741 75-79 29.204486277757287 22.265817086037284 21.413995430854456 27.115701205350973 80-84 29.74799458966877 21.233087694406784 22.16891512485665 26.850002591067796 85-89 28.809303869719677 22.453540361464505 21.613189574418197 27.12396619439762 90-94 29.10858121236054 22.87171257138855 21.270662423318544 26.749043792932365 95-99 29.280283719750926 22.683811265156674 20.843736633972114 27.19216838112028 100-104 29.32031501315232 23.54878754919337 20.7759206826639 26.35497675499041 105-109 29.71122897937779 23.451464888825207 20.853500593049816 25.983805538747184 110-114 30.780487828765825 23.480833320608568 20.834195834981802 24.904483015643805 115-119 29.71601398302242 24.27372849451919 20.011170712878357 25.999086809580028 120-124 30.35206394857129 24.600289156152826 19.791764052225954 25.255882843049932 125-129 30.467485837957568 25.21479476055621 19.252068775489672 25.06565062599655 130-134 30.875685417064062 25.24267662117331 19.551025704727923 24.33061225703471 135-139 30.290687087683814 26.089522843000033 20.04479580297453 23.57499426634162 140-144 31.2569802048801 26.9615131322353 19.878330839147644 21.90317582373695 145-149 31.06238096709945 28.046572799670916 19.575518225324945 21.31552800790469 150-151 30.874195552138552 29.37233476728333 17.997332455531634 21.756137225046484 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 647.0 1 492.5 2 307.0 3 282.0 4 292.5 5 321.0 6 363.0 7 391.0 8 434.0 9 487.0 10 544.0 11 592.5 12 607.5 13 630.0 14 719.0 15 752.5 16 726.0 17 720.5 18 733.0 19 744.0 20 768.5 21 845.5 22 977.5 23 1171.5 24 1528.5 25 1818.0 26 1922.5 27 2153.0 28 2903.0 29 3839.5 30 5447.0 31 6131.5 32 5573.5 33 6467.5 34 7795.5 35 10079.5 36 15996.5 37 24653.0 38 30458.0 39 36218.0 40 51680.0 41 72367.0 42 126922.5 43 211910.5 44 386676.0 45 608946.0 46 914487.5 47 1121491.5 48 1105789.0 49 985058.5 50 825528.0 51 893674.5 52 989528.5 53 984935.5 54 1002403.0 55 1028148.5 56 1010421.0 57 867760.5 58 836944.5 59 828680.5 60 786681.5 61 734790.5 62 577114.5 63 529482.5 64 444296.0 65 349434.5 66 339404.0 67 331454.0 68 324159.0 69 298927.0 70 268811.0 71 240112.5 72 190425.5 73 172841.0 74 197650.5 75 157119.5 76 123128.0 77 144885.0 78 141059.5 79 133613.0 80 143302.5 81 111561.5 82 166194.5 83 165509.0 84 83065.0 85 82822.5 86 71425.0 87 54278.0 88 44925.0 89 23304.5 90 2412.5 91 1376.5 92 1029.0 93 800.5 94 270.0 95 136.5 96 98.0 97 66.0 98 46.5 99 43.0 100 70.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0018425603399568335 2 0.004112381048599309 3 0.005202785114515793 4 0.00432601123294213 5 0.004468431355837345 6 0.0 7 0.0 8 1.3351886521426328E-5 9 0.003102088301811384 10-14 0.0026516846631552686 15-19 0.0044568597208521086 20-24 0.011699813095841845 25-29 0.019737648781740495 30-34 0.027995345532358627 35-39 0.03414611458989569 40-44 0.04799202091261479 45-49 0.04958000528289643 50-54 0.05730718707572989 55-59 0.055988910813181074 60-64 0.0584492184361959 65-69 0.055760148490780634 70-74 0.052692775093924955 75-79 0.05232693340323787 80-84 0.04812197927475668 85-89 0.0454364698324138 90-94 0.04195162745032152 95-99 0.0412858133757864 100-104 0.03349899315649056 105-109 0.029861939268054034 110-114 0.026596957950681246 115-119 0.020633115304444154 120-124 0.01928546489154819 125-129 0.016712111295985287 130-134 0.016261707657329174 135-139 0.014011469715584788 140-144 0.01522204076019411 145-149 0.011916113657488951 150-151 0.0089257361395735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 2.2468735E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 3.1785242332264922 #Duplication Level Percentage of deduplicated Percentage of total 1 72.10520236470174 2.2918813305790464 2 9.742125811680062 0.6193116595113277 3 4.434636865010591 0.4228680222298718 4 2.339333759714709 0.29742516179432166 5 1.6878725289329206 0.2682471867905286 6 1.4293137873416955 0.272586510597019 7 0.9240580941340164 0.2055998731579841 8 0.5778498553206061 0.1469367814642375 9 0.6020242316107419 0.17221937482478714 >10 3.6361509451559013 2.3132033749516574 >50 0.694899253911714 1.5703429607071009 >100 1.001044305631998 7.61437048781763 >500 0.2967412659679427 6.778293270801434 >1k 0.4105833783598187 28.992586590223457 >5k 0.07170377634477725 15.7994471463218 >10k+ 0.04645977618059529 32.23468026822756 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 154567 0.6879203479857677 Illumina Single End PCR Primer 1 (100% over 50bp) CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG 115495 0.5140253779307112 No Hit CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT 104531 0.46522868332373857 No Hit CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGG 78563 0.3496547535942722 No Hit CTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCG 76551 0.3407000883672356 No Hit CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC 74591 0.3319768558399038 No Hit CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG 73925 0.3290127370321471 No Hit CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC 73803 0.3284697603136091 No Hit CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT 70557 0.3140230190974258 No Hit CCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC 65981 0.2936569415234102 No Hit CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC 63028 0.2805142345574862 No Hit CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA 62789 0.2794505342646126 No Hit CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT 62760 0.27932146602823876 No Hit CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC 59726 0.26581825812623633 No Hit CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT 59107 0.263063318873982 No Hit CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC 56874 0.25312506467320034 No Hit GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA 56737 0.2525153285220552 No Hit GTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG 54279 0.24157568283216657 No Hit CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT 52126 0.23199347893862293 No Hit CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG 49138 0.21869499996328232 No Hit CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG 48401 0.2154148865078519 No Hit GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC 47087 0.20956676021146717 No Hit CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG 46169 0.20548108293591072 No Hit GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA 45670 0.20326021914451345 No Hit CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT 45185 0.20110166415688288 No Hit CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG 45157 0.2009770465493496 No Hit CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC 42831 0.1906248838664037 No Hit GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT 42732 0.19018427161119664 No Hit CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC 41155 0.18316562992976684 No Hit CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC 41019 0.1825603444074622 No Hit CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA 40502 0.1802593692969364 No Hit CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG 40476 0.180143652947084 No Hit GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG 39257 0.17471833639054446 No Hit ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG 38642 0.17198119965365205 No Hit CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT 38398 0.17089524621657606 No Hit CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA 37463 0.1667339082507315 No Hit GTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCG 36532 0.16259037280024888 No Hit CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC 35978 0.1601247244226255 No Hit CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA 35885 0.15971081594046127 No Hit CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT 35810 0.1593770187774256 No Hit CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA 35565 0.15828661471150912 No Hit GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT 35348 0.15732082825312593 No Hit CATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCC 35321 0.1572006612744331 No Hit CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGG 35320 0.15719621064559264 No Hit CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG 34014 0.1513836893799317 No Hit CTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCA 33183 0.14768521681349664 No Hit CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA 32173 0.14319008168461644 No Hit CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGG 31953 0.14221094333971182 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG 31910 0.14201956629957138 No Hit CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC 31121 0.13850802014443625 No Hit AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC 31033 0.1381163648064744 No Hit GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT 31012 0.13802290160082445 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 29885 0.1330070428976086 No Hit CTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT 29101 0.12951774988667586 No Hit CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA 28836 0.1283383332439499 No Hit CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC 28739 0.12790662224642374 No Hit CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT 28590 0.1272434785491929 No Hit CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC 28174 0.12539201695155514 No Hit CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG 28083 0.12498700972707186 No Hit AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG 28079 0.12496920721170995 No Hit CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA 27891 0.12413248898970057 No Hit CTTCCGTACGCCACATTTCCCGCGCCGCGCCGCGCGGCGGGGATTCGGCG 27711 0.12333137579841501 No Hit CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG 27634 0.12298867737769838 No Hit CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT 27597 0.12282400411060079 No Hit GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC 27596 0.12281955348176032 No Hit CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC 27563 0.12267268273002463 No Hit CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT 27532 0.12253471323596989 No Hit CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC 27285 0.12143540791237246 No Hit CTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT 26621 0.11848019036229676 No Hit AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA 26252 0.11683790832016133 No Hit CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCGTCTC 25717 0.11445682189050697 No Hit CGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT 25360 0.11286794739445723 No Hit GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTA 25163 0.11199117351288357 No Hit AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG 25068 0.1115683637730384 No Hit CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG 24759 0.11019311946133148 No Hit CGGGTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGA 24536 0.10920062922990548 No Hit AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC 24476 0.10893359149947696 No Hit GTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGC 24448 0.10880897389194362 No Hit CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCG 24248 0.10791884812384853 No Hit CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA 24230 0.10783873680471998 No Hit CTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGT 24122 0.10735806888994863 No Hit GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC 24046 0.1070198210980725 No Hit GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT 23938 0.10653915318330116 No Hit CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG 23885 0.10630326985475597 No Hit CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGTGGGTC 23829 0.10605403463968933 No Hit GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC 23689 0.10543094660202276 No Hit GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC 23061 0.10263595169020419 No Hit CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG 22977 0.10226209886760425 No Hit CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC 22895 0.10189714730268527 No Hit CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT 22866 0.10176807906631148 No Hit CTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGGGAGAGC 22864 0.10175917780863052 No Hit GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC 22558 0.10039728538344503 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC 22519 0.1002237108586665 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0011838672715664678 0.0 0.0 2 0.0012283735599712222 0.0 0.0 3 0.0013218367656212065 0.0 0.0 4 0.0013752443117069119 0.0 0.0 5 0.0014242012289521417 0.0 0.0 6 0.0015799732383687823 0.0 0.0 7 0.0016600845574973402 0.0 0.0 8 0.0017579983919877998 0.0 0.0 9 0.0019004185148830141 0.0 0.0 10-11 0.0020895702406032203 0.0 0.0 12-13 0.0023521573421912715 0.0 2.2253144202377214E-6 14-15 0.002906260632830464 0.0 4.450628840475443E-6 16-17 0.0037496547981005607 0.0 4.450628840475443E-6 18-19 0.00454186673170519 0.0 4.450628840475443E-6 20-21 0.0056701011427657145 0.0 4.450628840475443E-6 22-23 0.00832045061726884 0.0 4.450628840475443E-6 24-25 0.012379424119782444 0.0 4.450628840475443E-6 26-27 0.0189485522883242 0.0 4.450628840475443E-6 28-29 0.03107874119304002 0.0 4.450628840475443E-6 30-31 0.04978028358071783 0.0 4.450628840475443E-6 32-33 0.07453245587702201 0.0 4.450628840475443E-6 34-35 0.10264040231904467 0.0 4.450628840475443E-6 36-37 0.13592220478812 0.0 4.450628840475443E-6 38-39 0.1747739692510504 0.0 4.450628840475443E-6 40-41 0.2220218450215377 0.0 4.450628840475443E-6 42-43 0.28041187009415525 0.0 4.450628840475443E-6 44-45 0.34221107685857705 0.0 4.450628840475443E-6 46-47 0.4127579946089533 0.0 4.450628840475443E-6 48-49 0.5051174443064996 0.0 4.450628840475443E-6 50-51 0.5999736967835527 0.0 4.450628840475443E-6 52-53 0.7057050608323077 0.0 4.450628840475443E-6 54-55 0.8303070911646784 0.0 4.450628840475443E-6 56-57 0.9706977273086358 0.0 4.450628840475443E-6 58-59 1.121111179601344 0.0 4.450628840475443E-6 60-61 1.2880142117480133 0.0 4.450628840475443E-6 62-63 1.4700738604109223 0.0 4.450628840475443E-6 64-65 1.6812829026645248 0.0 4.450628840475443E-6 66-67 1.9078755435052308 0.0 4.450628840475443E-6 68-69 2.1620442806415223 0.0 4.450628840475443E-6 70-71 2.456789400916429 0.0 4.450628840475443E-6 72-73 2.7718293887039036 0.0 4.450628840475443E-6 74-75 3.0890946909116153 0.0 4.450628840475443E-6 76-77 3.437861544052213 0.0 4.450628840475443E-6 78-79 3.814398095842957 0.0 4.450628840475443E-6 80-81 4.222605322462524 0.0 4.450628840475443E-6 82-83 4.658624528706222 0.0 4.450628840475443E-6 84-85 5.135002037275352 0.0 4.450628840475443E-6 86-87 5.647089166346036 0.0 4.450628840475443E-6 88-89 6.183307604989778 0.0 4.450628840475443E-6 90-91 6.763932192889364 0.0 4.450628840475443E-6 92-93 7.4221423680505385 0.0 4.450628840475443E-6 94-95 8.104368136434918 0.0 4.450628840475443E-6 96-97 8.819742188423158 0.0 4.450628840475443E-6 98-99 9.596806406769229 0.0 4.450628840475443E-6 100-101 10.430086517999346 0.0 4.450628840475443E-6 102-103 11.276825330843057 0.0 4.450628840475443E-6 104-105 12.153734956596356 0.0 4.450628840475443E-6 106-107 13.094784819884165 0.0 4.450628840475443E-6 108-109 14.042210208985953 0.0 4.450628840475443E-6 110-111 15.08275610531701 0.0 4.450628840475443E-6 112-113 16.160475879038138 0.0 4.450628840475443E-6 114-115 17.20414834212963 0.0 4.450628840475443E-6 116-117 18.302594694360852 0.0 4.450628840475443E-6 118-119 19.553906795375887 0.0 4.450628840475443E-6 120-121 20.888979731168668 0.0 4.450628840475443E-6 122-123 22.248373128260226 0.0 4.450628840475443E-6 124-125 23.6938550390131 0.0 4.450628840475443E-6 126-127 25.31123358747166 0.0 4.450628840475443E-6 128-129 27.013988548977057 0.0 4.450628840475443E-6 130-131 28.581304198923526 0.0 4.450628840475443E-6 132-133 30.193813759430604 0.0 4.450628840475443E-6 134-135 31.79218367211149 2.2253144202377214E-6 4.450628840475443E-6 136-137 33.44334471878368 4.450628840475443E-6 4.450628840475443E-6 138-139 35.10165125005925 4.450628840475443E-6 4.450628840475443E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGCTT 4700 0.0 98.9961 2 AGAACCC 2235 0.0 93.07553 145 CATCTCC 1810 0.0 92.104416 145 CCTGGCC 11085 0.0 89.44567 5 CCCTCGT 1135 0.0 86.84103 1 CGGTGGG 3945 0.0 86.35391 145 CTCCGAT 835 0.0 85.92719 1 TGCTCCC 2040 0.0 85.27314 145 TGGCCCG 11685 0.0 84.84811 7 CCTCAAC 3080 0.0 79.29778 1 ACTTATG 8730 0.0 77.870575 6 CTTATGC 8925 0.0 76.90003 7 GCTTTTC 5835 0.0 76.01854 5 TTTTCTC 6035 0.0 73.61529 7 CCCCTGT 720 0.0 73.48053 1 GTAGGGG 6230 0.0 72.48218 145 TTATGCT 9545 0.0 72.056816 8 CATTCCT 3735 0.0 69.858604 4 TATGCTA 9935 0.0 69.81196 9 TCCACTC 7825 0.0 69.463684 2 >>END_MODULE