FastQCFastQC Report
Fri 3 Apr 2015
H2V3NBCXX l01n02 library 3 mfa.3420000000455d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2V3NBCXX l01n02 library 3 mfa.3420000000455d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22468735
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1545670.6879203479857677Illumina Single End PCR Primer 1 (100% over 50bp)
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG1154950.5140253779307112No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT1045310.46522868332373857No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGG785630.3496547535942722No Hit
CTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCG765510.3407000883672356No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC745910.3319768558399038No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG739250.3290127370321471No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC738030.3284697603136091No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT705570.3140230190974258No Hit
CCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC659810.2936569415234102No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC630280.2805142345574862No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA627890.2794505342646126No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT627600.27932146602823876No Hit
CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC597260.26581825812623633No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT591070.263063318873982No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC568740.25312506467320034No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA567370.2525153285220552No Hit
GTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG542790.24157568283216657No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT521260.23199347893862293No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG491380.21869499996328232No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG484010.2154148865078519No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC470870.20956676021146717No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG461690.20548108293591072No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA456700.20326021914451345No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT451850.20110166415688288No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG451570.2009770465493496No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC428310.1906248838664037No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT427320.19018427161119664No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC411550.18316562992976684No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC410190.1825603444074622No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA405020.1802593692969364No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG404760.180143652947084No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG392570.17471833639054446No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG386420.17198119965365205No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT383980.17089524621657606No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA374630.1667339082507315No Hit
GTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCG365320.16259037280024888No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC359780.1601247244226255No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA358850.15971081594046127No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT358100.1593770187774256No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA355650.15828661471150912No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT353480.15732082825312593No Hit
CATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCC353210.1572006612744331No Hit
CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGG353200.15719621064559264No Hit
CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG340140.1513836893799317No Hit
CTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCA331830.14768521681349664No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA321730.14319008168461644No Hit
CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGG319530.14221094333971182No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG319100.14201956629957138No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC311210.13850802014443625No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC310330.1381163648064744No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT310120.13802290160082445No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA298850.1330070428976086No Hit
CTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT291010.12951774988667586No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA288360.1283383332439499No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC287390.12790662224642374No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT285900.1272434785491929No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC281740.12539201695155514No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG280830.12498700972707186No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG280790.12496920721170995No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA278910.12413248898970057No Hit
CTTCCGTACGCCACATTTCCCGCGCCGCGCCGCGCGGCGGGGATTCGGCG277110.12333137579841501No Hit
CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG276340.12298867737769838No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT275970.12282400411060079No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC275960.12281955348176032No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC275630.12267268273002463No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT275320.12253471323596989No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC272850.12143540791237246No Hit
CTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAAT266210.11848019036229676No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA262520.11683790832016133No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCGTCTC257170.11445682189050697No Hit
CGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT253600.11286794739445723No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTA251630.11199117351288357No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG250680.1115683637730384No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG247590.11019311946133148No Hit
CGGGTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGA245360.10920062922990548No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC244760.10893359149947696No Hit
GTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGC244480.10880897389194362No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCG242480.10791884812384853No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA242300.10783873680471998No Hit
CTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGT241220.10735806888994863No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC240460.1070198210980725No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT239380.10653915318330116No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG238850.10630326985475597No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGTGGGTC238290.10605403463968933No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC236890.10543094660202276No Hit
GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC230610.10263595169020419No Hit
CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG229770.10226209886760425No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC228950.10189714730268527No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT228660.10176807906631148No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGGGAGAGC228640.10175917780863052No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC225580.10039728538344503No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC225190.1002237108586665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTT47000.098.99612
AGAACCC22350.093.07553145
CATCTCC18100.092.104416145
CCTGGCC110850.089.445675
CCCTCGT11350.086.841031
CGGTGGG39450.086.35391145
CTCCGAT8350.085.927191
TGCTCCC20400.085.27314145
TGGCCCG116850.084.848117
CCTCAAC30800.079.297781
ACTTATG87300.077.8705756
CTTATGC89250.076.900037
GCTTTTC58350.076.018545
TTTTCTC60350.073.615297
CCCCTGT7200.073.480531
GTAGGGG62300.072.48218145
TTATGCT95450.072.0568168
CATTCCT37350.069.8586044
TATGCTA99350.069.811969
TCCACTC78250.069.4636842
Produced by FastQC (version 0.11.2)