FastQCFastQC Report
Fri 3 Apr 2015
H2V3NBCXX l01n02 library 2 l4b.34200000004533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2V3NBCXX l01n02 library 2 l4b.34200000004533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22785819
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT3879891.7027652155053106No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG2644981.1608009350025996No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC2114490.9279850770340974No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC1994630.8753821839803081No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA1909050.8378237358946808No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC1896390.832267648575634No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC1762710.7735995796332799No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT1738350.7629087196734075No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC1731840.7600516795117174No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG1487900.6529938642977897No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC1118970.49108175571832635No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT1025810.45019667715257455No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG998500.4382111522960838No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA973350.4271735854655916No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA893460.3921123045873401No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC872410.3828741025284191No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG864970.3796089137722019No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA852690.37421959684661765No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG802050.35199524757042966No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA785190.3445959085341633No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA771750.33869750303906127No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG740900.3251583803066284No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC737260.3235608954850383No Hit
CCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATT698340.30648009623880534No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT660850.29002688031533996No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC656500.2881177981796485No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA643570.2824432161073517No Hit
CTGAAGTTTCCCTCAGGATAGCTGGAGTACGTTAAATAAAGCAGTTATAT639030.2804507487749288No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT623840.2737843217309854No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT617610.27105016501711No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC604160.26514737082744316No Hit
GTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTA594440.26088155971044974No Hit
CCCACTTGGAGCACACATTCAATGTCTATGCTTCATAAAAAATTTAAGCA572120.25108599344179816No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG567920.2492427417245788No Hit
CTCAGAATGAGGAGGTAGTGACGAAAAATAACGAGACCGTTCTCTTTGAG563660.24737315783997055No Hit
CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG562060.24667096670960126No Hit
CTCTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGG557340.24459950287501184No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT553820.24305468238819944No Hit
CTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAATATG548360.24065845515581422No Hit
CTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATC547240.24016692136455572No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTT519790.2281199547841576No Hit
CCCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCA517320.22703594722665008No Hit
CTGAGATAATGGTTAAGAGGGACGGACGGGGGCATTCGTATCGCTGCGTG516910.22685601074949294No Hit
CTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCC512700.22500837033770874No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG510140.22388486452911788No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA507350.22266041874553644No Hit
CTGTTATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAA507240.22261214310532354No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT504560.22143597296195497No Hit
GCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAA502540.22054945665986375No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT500730.21975510294363348No Hit
CTCGTTATTTTTCGTCACTACCTCCTCATTCTGAGAGTGGGTAATTTGCG497430.21830683373724682No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAAC489770.21494509370060386No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA483460.21217582743020996No Hit
CTGCTTACCAAAAATGGCCCACTTGGAGCACACATTCAATGTCTATGCTT480430.21084605297707315No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT476840.20927051162830707No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT470240.20637397321553375No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC464350.20378903211686183No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG459570.20169123611488357No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG458560.20124797796383798No Hit
CTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAA455950.20010252868242306No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG453650.19909312893251718No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC450150.19755708583483436No Hit
CTAGCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAA441870.19392324673517333No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT431160.1892229548562639No Hit
CTCCGGAGTCGAACCCTTATTCTCCGTTACCCGTTACAACCATGGTAGGC429620.18854709589328347No Hit
CTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAAAGAGGGTGGTAAACCTC424520.18630886166523133No Hit
CTCATGGTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTG423130.18569883312072302No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA414720.18200794099171946No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTT413760.1815866263134979No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC409230.17959854767563985No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA399530.17534151394777603No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA396790.17413901163701861No Hit
CCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTC392280.1721597103882902No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG391400.17177350526658708No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG390180.1712380845296805No Hit
GTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAA385560.1692105076407392No Hit
CTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACAACTTC375400.16475159396289418No Hit
CCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAGG370270.16250019365114768No Hit
ATCCGTTTCCATCTCAGCGGTTTCACGCCCTCTTGAACTCTCTCTTCAAA363590.1595685456818559No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG362240.1589760719156068No Hit
GTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGA359490.15776918091028458No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC356220.15633407778759237No Hit
CTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTT353820.15528079109203843No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA353560.15516668503335343No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC349780.15350775848785597No Hit
AAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAGC348870.15310838728245846No Hit
CTTCCGTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGA344950.1513880190130537No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG344560.15121685992502618No Hit
ATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGACGGACGGGG344200.15105886692069306No Hit
CTAATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGG342610.15036106448488862No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT341750.1499836367523151No Hit
CTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTCA339190.14886013094372424No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC337860.14827643456660478No Hit
CTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTA330410.14500685711582278No Hit
CTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGGTC330130.14488397366800818No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT326620.14334354187576054No Hit
CTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCG326090.1431109410638257No Hit
CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG322690.14161878491179097No Hit
AACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGAATAGC322130.14137301801616173No Hit
CCATGCACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTT320320.14057866429993146No Hit
CGGAGAATAAGGGTTCGACTCCGGAGAGGGAGCCTGAGAAACGGCTACCA319160.14006957573041373No Hit
CTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCA318000.13956048716089597No Hit
CTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTATCAACTTTCGATG317720.13943760371308137No Hit
AAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGC317550.13936299590547963No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG316610.13895045861638766No Hit
CTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTCAT314150.13787083975344488No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTAC310980.13647962357640075No Hit
GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC309340.13575987766777223No Hit
CGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAACGG306610.13456176405157963No Hit
CTCAGATCAGAAAACCAACAAAATAGAACCGAGATCCTATTTCATTATTC303950.1333943712973407No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA301690.13240252632569408No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA300960.1320821516224631No Hit
CCCCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAA299120.1312746318225384No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC297340.13049344419000256No Hit
CGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGC295910.12986586086723503No Hit
GTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTT290630.12754863013701637No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC289950.1272501989066094No Hit
ATCACTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAG286980.12594675662086144No Hit
CTCTTGCTAGAAACCTTCTAAAAAATTCAAAAGGATCGATAGGCCACGCT285100.1251216820426775No Hit
GGTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGT284610.12490663600900191No Hit
GTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAACTAG283460.124401936134049No Hit
CAAGTGTCTGCCTTATCAACTTTCGATGGTAGTTTATGTGCCTACCATGG278390.12217686798969131No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG278360.12216370190599689No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA277600.12183016111907148No Hit
GTAACATAACCCCGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTA277390.12173799853321049No Hit
GTGGCTAGCAAGTTTACCTTGAAAAAATTAGAGTGCTCAATGCGGGCTAA276500.1213474047169426No Hit
GTAACTATTTAGTAGGCTAGAGTCTCGCTCGTTATCGGAATAAACCAGAC276030.1211411360723966No Hit
AGGTGCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGG274550.12049160927680501No Hit
CTCGTAGTTGGATCTGCGTCTCAGGACCTGGTCCATCCATTGGATGAGAA273790.12015806848987962No Hit
CTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAA273530.12004396243119458No Hit
CTCCTCATTCTGAGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT272550.1196138703638434No Hit
CCCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAAT270400.11867030103240968No Hit
ATCCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAA267700.11748535349991152No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT264900.11625651902176526No Hit
CACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAAT264880.11624774163263563No Hit
ATCAAGTGTCTGCCTTATCAACTTTCGATGGTAGTTTATGTGCCTACCAT260870.1144878751121476No Hit
CTCAGGATAGCTGGAGTACGTTAAATAAAGCAGTTATATTCGGTAAAGCG259950.11408411521218527No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA259850.11404022826653717No Hit
CTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTG257160.11285966942860381No Hit
GTCAGAATCGCTTCGGACTTTCACCAGAGTTTCCTCTGGCTTCATCCTGC254860.11185026967869796No Hit
CGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGC254230.11157378192111506No Hit
GTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGT253970.11145967586243005No Hit
GTAAAAATATGTTACGGATAGTTATACCTATACGCTACATTTCCATATAG252840.11096375337660674No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT252220.11069165431358864No Hit
GTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTGCAATCT251280.11027911702449669No Hit
CTCCAGCTATCCTGAGGGAAACTTCAGAGGGAACCAGCTACTAGATAGTT244300.10721580821826066No Hit
CTCAGGACCTGGTCCATCCATTGGATGAGAACTAGGCTCCTAGGCTAATA243350.1067988822346039No Hit
ATTTTAATCCGTTAATTGGTGACTCTGAATAGCTATGGCTGATCGCATGG242840.10657505881179868No Hit
CACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGA240650.10561393470210573No Hit
CTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGT240240.1054339982249486No Hit
CTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGG235220.10323087355341497No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA233680.1025550145904345No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC233490.10247162939370316No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA230320.101080413216659No Hit
CTCTCATAGATCCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACC229590.10076003851342802No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG228170.10013684388522529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGC510600.0113.8833164
CACCTGG514800.0112.979873
CCTGGCC527600.0110.213845
CTCCTCG11800.0105.0251851
TGGCCCG564350.0103.0978557
GCCCGGA570100.0102.083679
CTGGCCC573950.0101.196636
TTAGATT269300.099.789012
CCCTCGT17000.098.0524141
AGATTAA276450.096.269834
TAGATTA281250.095.037063
CTCCCAC130450.093.72391
GTAGGGG63400.091.57279145
CCTCAAC116750.091.251451
CCAGACG325200.090.505156
CAGACGT328850.089.654887
TGGCTCA249050.089.13643
CCCCTGT7450.085.606311
AGTCCCT9050.084.08531
CAACCAG349950.084.063543
Produced by FastQC (version 0.11.2)