FastQCFastQC Report
Fri 3 Apr 2015
H2V3NBCXX l01n02 library 1 l4a.3420000000450c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2V3NBCXX l01n02 library 1 l4a.3420000000450c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26046660
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10955394.206063272603858Illumina Single End PCR Primer 1 (100% over 50bp)
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2482510.9531010885848704No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC1420570.5453943039145902No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG1164400.44704388201788636No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC1086100.41698244611785157No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1005850.3861723537681991No Hit
CCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATT976170.3747774186786329No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG920710.35348486139873597No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA827680.3177681898562042No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC818880.31438963767331396No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG799530.30696066213479967No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA787560.30236506331330004No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC755020.28987209876429454No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG692610.26591125311268315No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT684870.2629396628972774No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT656370.2519977609413261No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC650340.24968268484327744No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA599370.23011395702942336No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT594840.2283747705080037No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT555530.21328262433647924No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC547330.2101344279842406No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG509420.19557977875090318No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC508100.19507299592346963No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC504200.1935756830242342No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG484950.1861851001241618No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA480350.1844190387558328No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG475200.18244181787607316No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA469420.18022272337412934No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC468520.17987718962815194No Hit
GTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTA459930.17657926198598975No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC455020.17469418343849077No Hit
GCGTAACATAACCCCGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCG452090.17356927913214207No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT452000.17353472575754436No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG444130.1705132251121641No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG420030.16126059924765787No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC417090.160131855677465No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC416060.15973641150151305No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT412270.15828133050456375No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC407280.1563655378463112No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT389980.14972361139585652No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA389750.14963530832744007No Hit
CGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAACGG388080.14899415126545976No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC387710.1488520985032246No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG382120.14670595001432046No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT374350.14372284200738214No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG372110.14286284690628281No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC371140.14249043831339603No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA361790.13890072661907515No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT354690.13617484928969778No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC350150.13443182350443397No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA344880.13240853145854403No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA344810.13238165661163465No Hit
CCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTC338530.12997059891748117No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT330580.12691838416134738No Hit
GCTAAATTGATTGGTTGAATACCGATAAGTTTTGTTAACTCTATCCGTTT329950.12667651053916318No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA327040.12555928476050288No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC323380.12415411419352806No Hit
CTCATGGTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTG321770.12353599271461294No Hit
GCGGTGTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTT318570.12230742828447103No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG317960.12207323318997522No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT313920.12052217059692107No Hit
CACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGA313640.12041467120928365No Hit
CGCCGACGGAACGCTAGTTGGTATCGTTTACGGTCAGAACTAGGGCGGTA312870.12011904789328075No Hit
GGGAAAACTGGCAATATTAGCCTAGGAGCCTAGTTCTCATCCAATGGATG312390.11993476322875946No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT311790.11970440739810786No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC311350.11953547978896335No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA311060.11942414113748175No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC310310.11913619634916722No Hit
CTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTT307670.11812263069430015No Hit
GTGAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCA297310.11414515335171573No Hit
GAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAG297010.11402997543638993No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT295780.11355774598355411No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA290110.11138088338389644No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA290040.11135400853698708No Hit
CGGGGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCG289470.11113517049786804No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG284540.10924241342268068No Hit
CCCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCA284400.10918866372886196No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT283480.10883545145519617No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG282810.10857822077763521No Hit
GTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCTGTAACCA282370.1084092931684907No Hit
CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG279940.1074763520543517No Hit
GGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCT276350.10609805633428623No Hit
CCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAGG270040.10367548084860016No Hit
GGTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGT266180.10219352500474149No Hit
CCCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCAT264220.10144102929127957No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA263250.1010686206983928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGC418650.088.257114
CCTGGCC431100.085.775535
CACCTGG432200.085.589113
CCTCAAC172650.079.883851
TGGCCCG467250.079.2145547
CTGGCCC479100.077.20996
GCCCGGA486450.076.296579
TGGCTCA263550.066.082033
CTTGTTA78850.065.167541
CCAGACG332900.064.702866
GGCGGTT81500.064.6513754
GTAGGGG65800.064.55009145
CTCCCAC198350.064.016021
CCCTCGT18500.063.856141
CAGACGT338750.063.7994277
CTGTCGG17750.061.2462731
TCTCGGG55500.058.637913145
GGTTATA89350.058.5640457
TCCAATC25700.057.246052
CAACCAG381050.056.4894183
Produced by FastQC (version 0.11.2)