FastQCFastQC Report
Fri 3 Apr 2015
H2V3NBCXX l01n01 library 2 l4b.34100000004536.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2V3NBCXX l01n01 library 2 l4b.34100000004536.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22785819
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT3645241.5997844975420898No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG2669071.1713733002092224No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC2494811.0948959087228771No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC1976650.8674913111527831No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC1920790.8429760633137655No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA1819580.7985580856233432No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC1801590.7906628241012535No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT1763090.7737663500267425No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC1710740.7507915339799723No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG1614000.7083353027600192No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG1148850.5041951750779728No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT1131290.4964886274221699No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC1039120.4560380296183341No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA955230.41922127091415934No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC906530.39784832838354417No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG889570.39040510240162973No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA869720.38169354369048575No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA837920.3677374949743961No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA811690.3562259491309046No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG790060.3467332027872248No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG748050.3282962969204662No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC698170.30640548843120363No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC667080.2927610370292154No Hit
CTGAAGTTTCCCTCAGGATAGCTGGAGTACGTTAAATAAAGCAGTTATAT653840.2869504054254095No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT647870.2843303547702192No Hit
CCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATT630070.2765184784448608No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT625820.2746532832548174No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT610470.26791663709783703No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC603070.26466900311987906No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT596900.2619611785733925No Hit
CTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATC575480.2525605948155737No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG564030.24753553953886848No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTT557390.2446214463478359No Hit
CCCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCA555760.24390608913377218No Hit
CTCAGAATGAGGAGGTAGTGACGAAAAATAACGAGACCGTTCTCTTTGAG553130.2427518624632277No Hit
CCCACTTGGAGCACACATTCAATGTCTATGCTTCATAAAAAATTTAAGCA546170.2396973310461213No Hit
CTCTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGG545620.2394559528450568No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT543450.2385036061244935No Hit
GTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTA539020.23655941443228354No Hit
CTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAATATG530960.23302212661304822No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG528160.23179329213490196No Hit
CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG517360.22705350200490929No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA515060.22604410225500346No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT506090.22210744323037063No Hit
CTCGTTATTTTTCGTCACTACCTCCTCATTCTGAGAGTGGGTAATTTGCG505610.22189678589125983No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAAC497160.218188338983997No Hit
CTGTTATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAA487970.2141551286789384No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA483610.21224165784868212No Hit
CTGAGATAATGGTTAAGAGGGACGGACGGGGGCATTCGTATCGCTGCGTG481610.21136391893572049No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT472490.20736142949261557No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA468140.20545234735692405No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT455820.20004547565308056No Hit
GCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAA455650.19997086784547882No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTT452160.1984392134423608No Hit
CTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAA451560.1981758917684723No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC449520.19728059807725146No Hit
CTGCTTACCAAAAATGGCCCACTTGGAGCACACATTCAATGTCTATGCTT447600.19643796872080835No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT444390.19502919776550495No Hit
CTCCGGAGTCGAACCCTTATTCTCCGTTACCCGTTACAACCATGGTAGGC443330.19456399614163528No Hit
CTAGCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAA436400.1915226308082233No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG429450.1884724880856817No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG428940.1882486646628765No Hit
CCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTC426000.18695838846082294No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA425550.18676089720540656No Hit
CTCATGGTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTG419720.18420228827412347No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC418070.18347815367093015No Hit
CTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACAACTTC415410.1823107609166912No Hit
CTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAAAGAGGGTGGTAAACCTC415290.1822580965819135No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA414730.18201232968628425No Hit
CTAATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGG411080.18041045617012932No Hit
GTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAACTAG408850.17943177728217713No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG396620.17406440382941687No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA396020.1738010821555284No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG395260.17346754136860298No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA386840.1697722605450346No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC383040.16810455661040755No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA379270.1664500187594749No Hit
CCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAGG377750.1657829371856241No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA376270.1651334103900325No Hit
CGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAACGG373000.16369830726734028No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG369370.16210521114031495No Hit
CTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTT360250.15810272169721No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT352720.15479803468990955No Hit
CTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGGTC352220.15457859996166914No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG348070.15275729171727379No Hit
ATCCGTTTCCATCTCAGCGGTTTCACGCCCTCTTGAACTCTCTCTTCAAA345960.1518312771640993No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG344230.1510720330043875No Hit
ATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGACGGACGGGG340100.1492595021491218No Hit
AAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAGC339250.1488864631111131No Hit
CTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCG338740.1486626396883079No Hit
CTTCCGTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGA337360.14805699983836437No Hit
GTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAA335720.14733725392973587No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG330620.14509901970168376No Hit
CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG330590.1450858536179893No Hit
CCATGCACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTT330230.14492786061365623No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC328730.14426955642893505No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC325880.14301877847796474No Hit
CTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTATCAACTTTCGATG321530.14110969634227324No Hit
CGGAGAATAAGGGTTCGACTCCGGAGAGGGAGCCTGAGAAACGGCTACCA320930.14084637466838476No Hit
GGTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGT316290.13881002039031382No Hit
CTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCC315770.13858180827294378No Hit
CTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCA314880.1381912144566759No Hit
GTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGA313960.13778745455671354No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT311550.1367297791665948No Hit
CTCTTGCTAGAAACCTTCTAAAAAATTCAAAAGGATCGATAGGCCACGCT309040.13562821683082799No Hit
CTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAA308150.13523762301456008No Hit
CTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTCA306210.1343862162689873No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA304070.13344703563211838No Hit
CTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTCAT303860.1333548730462574No Hit
CGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGC303750.13330659740604453No Hit
CAAGTGTCTGCCTTATCAACTTTCGATGGTAGTTTATGTGCCTACCATGG302180.13261757235936966No Hit
ATCACTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAG302180.13261757235936966No Hit
GTAACTATTTAGTAGGCTAGAGTCTCGCTCGTTATCGGAATAAACCAGAC301730.1324200811039533No Hit
GTGGCTAGCAAGTTTACCTTGAAAAAATTAGAGTGCTCAATGCGGGCTAA299670.13151601002360283No Hit
CTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTA295830.12983075131071656No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTAC294320.12916805843143053No Hit
ATCCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAA294200.12911539409665285No Hit
GTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTT292000.12814988129239507No Hit
AAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGC290000.12727214237943346No Hit
GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC284670.12493296817639077No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC284480.12484958297965941No Hit
CTCCTCATTCTGAGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT281360.12348031027543929No Hit
CTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTG279960.12286589303636616No Hit
AACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGAATAGC279570.12269473394833866No Hit
GTAACATAACCCCGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTA271740.11925838610409396No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT269700.11836309241287311No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC268590.11787594731617942No Hit
ATCAAGTGTCTGCCTTATCAACTTTCGATGGTAGTTTATGTGCCTACCAT267890.11756873869664286No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG267230.11727908485536552No Hit
ATGGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATC266000.11673927542389415No Hit
CTCAGATCAGAAAACCAACAAAATAGAACCGAGATCCTATTTCATTATTC265970.1167261093401997No Hit
CTCGTAGTTGGATCTGCGTCTCAGGACCTGGTCCATCCATTGGATGAGAA262940.11539633488706286No Hit
GTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTGCAATCT262700.11529100621750749No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA262670.11527784013381306No Hit
CCCCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAA261460.11474680809147128No Hit
AGGTGCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGG254730.11179321664935547No Hit
CTCAGGATAGCTGGAGTACGTTAAATAAAGCAGTTATATTCGGTAAAGCG253130.11109102551898617No Hit
CTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGT250960.11013867879842282No Hit
CTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGG249910.10967786586911799No Hit
CGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGC249520.10950670678109047No Hit
ATTTTAATCCGTTAATTGGTGACTCTGAATAGCTATGGCTGATCGCATGG248470.10904589385178562No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA245700.10783022545733378No Hit
CTCCAGCTATCCTGAGGGAAACTTCAGAGGGAACCAGCTACTAGATAGTT245680.10782144806820418No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC245120.10757568117257493No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA244610.10735185774976971No Hit
GTCAGAATCGCTTCGGACTTTCACCAGAGTTTCCTCTGGCTTCATCCTGC244050.10710609085414047No Hit
CTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTA244040.10710170215957565No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA240250.10543838691951339No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT239170.10496440790651414No Hit
CTCAGGACCTGGTCCATCCATTGGATGAGAACTAGGCTCCTAGGCTAATA239020.10489857748804202No Hit
CACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGA238400.1046264784250239No Hit
GTAAAAATATGTTACGGATAGTTATACCTATACGCTACATTTCCATATAG238360.10460892364676468No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT237970.10443776455873717No Hit
GTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGT237900.10440704369678352No Hit
CTCTCATAGATCCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACC237550.10425343938701523No Hit
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGC236860.10395061946204347No Hit
TTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTC236620.10384529079248807No Hit
CCCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAAT235720.10345030828165536No Hit
ATCAACTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCA234600.10295877449039686No Hit
CACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAAT234050.10271739628933241No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA232280.10194059735136138No Hit
CGAACCCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACT231400.10155439222965829No Hit
GTAAACGATACCAACTAGCGTTCCGTCGGCGGTAAATACGCCTTGACGGG230220.10103652627101094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTCG11350.0113.7920151
ACCTGGC488400.0112.604764
CACCTGG492950.0111.493343
CCTGGCC506850.0108.4056855
CCCTCGT15550.0103.588191
TGGCCCG536300.0101.815977
GCCCGGA541950.0100.832999
CTGGCCC550500.099.9200066
TTAGATT256800.099.0917362
AGATTAA265450.095.5337144
TAGATTA270350.094.0447853
CTCCCAC129250.093.30141
CCTCAAC105800.092.995451
TGGCTCA271450.091.046333
CCAGACG299800.090.794756
CAGACGT303550.089.619827
GTCCTCG7650.089.156721
ACTTATG140150.085.188896
CTTGTTA39450.085.157251
CCCCTGT7400.084.324621