FastQCFastQC Report
Fri 27 Dec 2024
H2TFGDSXF_n02_R4CRISPR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2TFGDSXF_n02_R4CRISPR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154462074
Sequences flagged as poor quality0
Sequence length90
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC10863624070.33198324140074No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAA2617131016.94351844582897No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGTCTAAA38565232.4967442817063303No Hit
CAAGTTGATAACGGACTAGCCTTATTTAACTTGCTATTTCTAGCTCTAAA5176030.3351003819876198No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTCC3448300.22324573992189176No Hit
CAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA3155110.20426438142996833No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTTTCTAGCT2979780.19291337496866706No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTCTAGCTCTAAA2661360.17229860580533185No Hit
CAAGTTGATACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2337290.15131805105763374No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTTTAGCTCTAA2133980.13815559669359354No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1991710.12894492145690079No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAAATTGCTATTTCTAGCTCTAA1662310.10761929818448508No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCCCC1602490.10374650284703546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)