FastQCFastQC Report
Fri 27 Dec 2024
H2TFGDSXF_n02_R3CRISPR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2TFGDSXF_n02_R3CRISPR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118444899
Sequences flagged as poor quality0
Sequence length90
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC8908231175.20991765124474No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAA1565607113.21802047380698No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGTCTAAA23457801.9804820805326535No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTTCAGC5206040.4395326471594188No Hit
CAAGTTGATAACGGACTAGCCTTATTTAACTTGCTATTTCTAGCTCTAAA3198600.2700496202879957No Hit
CAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2615080.22078451854646775No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1988140.1678535772148364No Hit
CAAGTTGATACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1948730.1645262916725523No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTCC1741720.14704896662540107No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTTTAGCTCTAA1536760.12974471783711006No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTTTCTAGCT1370730.11572722941829686No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTCTAGCTCTAAA1337870.11295294362993209No Hit
CAAGTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1240980.10477276864409332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)