FastQCFastQC Report
Fri 27 Dec 2024
H2TFGDSXF_n02_R2CRISPR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2TFGDSXF_n02_R2CRISPR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176673463
Sequences flagged as poor quality0
Sequence length90
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC12220357369.1691728485562No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAA3004460417.005725415593396No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGTCTAAA59576243.372110275553947No Hit
CAAGTTGATAACGGACTAGCCTTATTTAACTTGCTATTTCTAGCTCTAAA5908250.33441638034796434No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTCC4055920.22957154578443964No Hit
CAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA3615080.2046192981455285No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTTTAGCTCTAA2957590.16740431470458017No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTTTCTAGCT2952520.16711734461219No Hit
CAAGTTGATACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2649440.14996253285644828No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTCTAGCTCTAAA2352940.13318015960325633No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2301590.13027366764186876No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGT2138260.12102892894559948No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAAATTGCTATTTCTAGCTCTAA2103450.11905862738423824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)