FastQCFastQC Report
Fri 27 Dec 2024
H2TFGDSXF_n02_R1CRISPR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2TFGDSXF_n02_R1CRISPR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154301688
Sequences flagged as poor quality0
Sequence length90
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC10239010966.35708936638464No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAA2664688617.26934186228734No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGTCTAAA40015112.5933034510938078No Hit
CAAGTTGATAACGGACTAGCCTTATTTAACTTGCTATTTCTAGCTCTAAA5589450.36224166257986756No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTCC4748570.30774582323428634No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGGTGTTTCCAGC3667060.23765520957878308No Hit
CAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA3069830.1989498650202712No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTTTCTAGCT2944340.19081709592185409No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2688670.1742476077124963No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTTTAGCTCTAA2674600.17333575767492576No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTCTAGCTCTAAA2443310.15834629106585016No Hit
CAAGTTGATACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2253360.1460359915181226No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCCCC1952300.1265248634221033No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAAATTGCTATTTCTAGCTCTAA1909940.12377959209363933No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATCCCATATAAGAA1802410.11681077656130372No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCCCCATATAA1792840.1161905629963037No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATACTGTTTCCAGC1644610.10658405758983013No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAC1574800.10205980377868583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)