FastQCFastQC Report
Fri 27 Dec 2024
H2TFGDSXF_n02_ES1CRISPR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2TFGDSXF_n02_ES1CRISPR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128163093
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC12153918794.8316587521807No Hit
CAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA3658370.28544645064082524No Hit
CAAGTTGATACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2704150.21099287920587245No Hit
CAAGTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1735520.13541495912555732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1685460.13150899846026656No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTTCAGC1654720.129110492050937No Hit
AAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1573960.12280914599962096No Hit
CAAGTTATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1402040.10939498783787933No Hit
CAAGTTGTAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1287720.10047510323428291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)