FastQCFastQC Report
Fri 27 Dec 2024
H2TFGDSXF_n02_D4GEX.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2TFGDSXF_n02_D4GEX.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646293771
Sequences flagged as poor quality0
Sequence length90
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92826801.436294208071518Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16255630.2515207592802252Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9319250.1441952625596325Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6694380.10358106948241035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)