FastQCFastQC Report
Fri 27 Dec 2024
H2TFGDSXF_n02_D4CRISPR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2TFGDSXF_n02_D4CRISPR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140598870
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC13273938394.40999276878968No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAA5084020.3615975007480501No Hit
CAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA3981260.2831644379503192No Hit
CAAGTTGATACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2918200.20755501093287593No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATACTGTTTCCAGC2604210.1852226835108988No Hit
CAAGTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1893350.13466324444855068No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1813010.12894911602063375No Hit
AAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1695280.12057564900770538No Hit
CAAGTTATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1527590.1086488106198862No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCTAGC1518460.10799944551474702No Hit
CAAGTTGTAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1411200.10037065020508343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)