FastQCFastQC Report
Fri 27 Dec 2024
H2TFGDSXF_n02_D3CRISPR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2TFGDSXF_n02_D3CRISPR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129865608
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC12274306794.51545246683017No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAA4151500.31967663062879587No Hit
CAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA3648640.28095506240574486No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTTCAGC2746240.2114678429719437No Hit
CAAGTTGATACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2692680.207343579371684No Hit
CAAGTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1725680.13288198673816703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1622870.12496534109323232No Hit
AAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1563500.12039369191572259No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATTCTGTTTCCAGC1411090.10865771328772433No Hit
CAAGTTATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1388630.10692823306999032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)