FastQCFastQC Report
Fri 27 Dec 2024
H2TFGDSXF_n02_D1CRISPR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2TFGDSXF_n02_D1CRISPR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103317008
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC9735458994.22900535408458No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAA3561940.34475833833670444No Hit
CAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2887600.2794893169960942No Hit
CAAGTTGATACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2131800.20633582420427815No Hit
CAAGTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1373830.13297229822992937No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1334640.1291791183112852No Hit
AAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1237720.11979828142138998No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATTCTGTTTCCAGC1141550.11049003664527335No Hit
CAAGTTATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1106710.10711789098654502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)