FastQCFastQC Report
Tue 1 May 2018
H2T2LBGX5_n02_4_14hpc-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2T2LBGX5_n02_4_14hpc-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53503297
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1949380.36434764010898246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG694350.037.56948569
CTCGGTG1754500.033.10701469
TCTCGGT2330000.024.66634268
ATCTCGG3134750.018.09660767
GTAGATA133900.017.64998869
CTAGGTG40750.017.01778269
AGATCTC3473900.016.4081765
TAGATCT3828250.014.81396764
GATCTCG3993050.014.31979966
GAGTGTT91100.014.2019369
AGTGTAT68500.013.24645469
TCTAGGT56150.012.96416168
GTAGATC4407250.012.91726463
TGTAGAT5043550.011.40737162
TCTCGGG291450.010.92627569
ATCTAGG69600.010.06188767
GTGTAGA5878450.09.91043561
AGTGTAC35550.09.80203569
TAGCTCT246650.09.79128364
AGTGTAG6988800.09.74197569
Produced by FastQC (version 0.11.2)