FastQCFastQC Report
Tue 1 May 2018
H2T2LBGX5_n02_3_14hpc-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2T2LBGX5_n02_3_14hpc-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123423159
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16041991.2997552590596064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG1269450.036.88645669
TCGGTGG602050.032.73937269
TCTCGGT2084950.023.72638769
ATCTCGG2606350.019.1034368
GATCTCG3153050.015.84730267
AGTGTAG10354700.014.13588669
GAGTGTA12772700.013.43871669
GTGTAGA8113250.013.39791169
AGATCTC3895200.012.9403166
CGCCGTA154850.012.87860145
GCCGTAT168500.012.12231346
TGTAGAT6684800.012.08287369
CGAGTGT15240300.012.07734569
GTGTATG1049950.012.0758171
GTAGATC5258700.011.04060369
GGTCGCC204350.010.90763942
TGGTCGC221350.010.84935241
GAGTATA1066350.010.519793511
TAGATCT4827450.010.50138765
TGAGTAT1117500.010.45460410
Produced by FastQC (version 0.11.2)