FastQCFastQC Report
Tue 1 May 2018
H2T2LBGX5_n02_2_14hpc-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2T2LBGX5_n02_2_14hpc-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60765902
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7620321.2540454019756013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG549500.034.76561769
TCGGTGG286000.030.593369
TCTCGGT912250.022.54490369
ATCTCGG1114800.018.46336268
GATCTCG1346100.015.39751467
AGTGTAG4596600.014.6418869
GTGTAGA3578950.013.93013569
GAGTGTA5699100.013.81262869
TGTAGAT2933300.012.61137569
CGCCGTA74550.012.588490545
AGATCTC1692150.012.358623566
CGAGTGT6846750.012.32327969
GTGTATG615800.012.0257281
GTAGATC2264350.011.49174869
GCCGTAT82300.011.10990246
GAGTATA627150.010.81897811
GGTCGCC92850.010.813848542
TGAGTAT657750.010.70858610
TTGAGTA681000.010.5759979
TGTATGA695900.010.575142
Produced by FastQC (version 0.11.2)