FastQCFastQC Report
Tue 1 May 2018
H2T2LBGX5_n02_1_14hpc-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2T2LBGX5_n02_1_14hpc-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81009747
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31695153.912510676030133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA173000.028.69894845
CTCGGTG591750.028.05596769
GGTCGCC195400.027.22920642
TGGTCGC202700.026.36778541
GCCGTAT186300.026.33598746
CCGTATC212550.022.98605547
CGTATCA226550.021.8852148
GTCGCCG251800.021.39046543
TCGCCGT248000.020.21456744
GTGGTCG276150.020.11641940
TCTCGGT942650.019.69474869
GGTGGTC286250.019.45489739
AAGGGGG241400.018.57796365
CGGTGGT315100.017.84877838
AGGGGGG277150.017.7499266
ATCTCGG1119400.016.56868268
TCGGTGG375800.016.3969369
GATCTCG1317600.014.15156367
AGTGTAG4011750.013.62945669
GAGTGTA4912150.013.26356669
Produced by FastQC (version 0.11.2)