FastQCFastQC Report
Tue 1 May 2018
H2T2LBGX5_n01_4_14hpc-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2T2LBGX5_n01_4_14hpc-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53503297
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1307080.24429896348256822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG158150.038.18687440
AAGGGGG142100.038.10224569
GTATGCC172600.035.85114342
CGTATGC177100.034.86201541
AAAGGGG156350.034.43122568
AAAAGGG181500.029.85044567
AAAAAGG213600.025.78420366
TGAAAAA242000.025.4372159
TTGAAAA238700.025.35461658
GAAAAAA250550.024.69380860
CCGTCTT250850.024.33770847
CTTGAAA247550.024.3219857
AAAAAAG247050.023.0463965
ATCTCGT652050.022.13221269
GGATCTC1487950.022.08106268
GTCTTCT282250.021.50680549
TCTGCTT280750.021.44653753
TATGCCG300350.020.70581243
TCTTCTG320150.019.09001450
TTCTGCT315150.018.9202952
Produced by FastQC (version 0.11.2)