Basic Statistics
Measure | Value |
---|---|
Filename | H2T2LBGX5_n01_3_14hpc-1000.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 123423159 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 771283 | 0.6249094628990982 | RNA PCR Primer, Index 39 (96% over 30bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 341567 | 0.2767446585936113 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGGGGG | 131920 | 0.0 | 56.250355 | 69 |
TATGCCG | 164200 | 0.0 | 51.265877 | 43 |
CGTATGC | 166640 | 0.0 | 50.991177 | 41 |
ATGCCGT | 165585 | 0.0 | 50.606255 | 44 |
GTATGCC | 167285 | 0.0 | 50.43987 | 42 |
GCCGTCT | 164825 | 0.0 | 50.20137 | 46 |
TGCCGTC | 167105 | 0.0 | 50.045727 | 45 |
TCGTATG | 171555 | 0.0 | 49.341022 | 40 |
CCGTCTT | 176075 | 0.0 | 46.85401 | 47 |
CTGCTTG | 183960 | 0.0 | 44.72512 | 54 |
AAAGGGG | 169470 | 0.0 | 43.553097 | 68 |
CGTCTTC | 193140 | 0.0 | 42.892185 | 48 |
CTCGTAT | 194645 | 0.0 | 42.193848 | 39 |
CTTCTGC | 202530 | 0.0 | 40.838207 | 51 |
TGCTTGA | 203375 | 0.0 | 40.588055 | 55 |
GCTTGAA | 208875 | 0.0 | 39.52585 | 56 |
TCTCGTA | 197130 | 0.0 | 39.51566 | 38 |
ATCTCGT | 191060 | 0.0 | 39.18123 | 37 |
TCTGCTT | 214705 | 0.0 | 38.374557 | 53 |
TTCTGCT | 215090 | 0.0 | 38.256348 | 52 |