FastQCFastQC Report
Tue 1 May 2018
H2T2LBGX5_n01_3_14hpc-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2T2LBGX5_n01_3_14hpc-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123423159
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG7712830.6249094628990982RNA PCR Primer, Index 39 (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3415670.2767446585936113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG1319200.056.25035569
TATGCCG1642000.051.26587743
CGTATGC1666400.050.99117741
ATGCCGT1655850.050.60625544
GTATGCC1672850.050.4398742
GCCGTCT1648250.050.2013746
TGCCGTC1671050.050.04572745
TCGTATG1715550.049.34102240
CCGTCTT1760750.046.8540147
CTGCTTG1839600.044.7251254
AAAGGGG1694700.043.55309768
CGTCTTC1931400.042.89218548
CTCGTAT1946450.042.19384839
CTTCTGC2025300.040.83820751
TGCTTGA2033750.040.58805555
GCTTGAA2088750.039.5258556
TCTCGTA1971300.039.5156638
ATCTCGT1910600.039.1812337
TCTGCTT2147050.038.37455753
TTCTGCT2150900.038.25634852