FastQCFastQC Report
Tue 1 May 2018
H2T2LBGX5_n01_2_14hpc-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2T2LBGX5_n01_2_14hpc-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60765902
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG3627100.5968972533313173TruSeq Adapter, Index 1 (96% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1667480.2744104744795856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG603800.057.23100769
TATGCCG783000.050.0047843
ATGCCGT787900.049.52341544
CGTATGC802050.049.1652241
TGCCGTC794300.048.96016345
GTATGCC806000.048.69333342
GCCGTCT797550.048.43565846
TCGTATG826150.047.3197840
CCGTCTT867850.044.4760947
CTGCTTG881550.043.50140454
AAAGGGG797100.043.2575568
CTCGTAT907650.040.57290339
CGTCTTC963750.040.14829348
CTTCTGC981200.039.42526651
TGCTTGA999700.038.51385555
TCTCGTA915400.037.91864838
ATCTCGT876650.037.8119737
GCTTGAA1031000.037.52036756
TTCTGCT1043400.036.90497652
TCTGCTT1046000.036.81157753
Produced by FastQC (version 0.11.2)