FastQCFastQC Report
Tue 1 May 2018
H2T2LBGX5_n01_1_14hpc-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2T2LBGX5_n01_1_14hpc-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81009747
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG18554502.2904034004698226RNA PCR Primer, Index 27 (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2819590.348055648167868No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG2092890.2583503933174856RNA PCR Primer, Index 27 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG2695150.063.23082769
TATGCCG3438350.055.49230643
TGCCGTC3426750.055.44519445
ATGCCGT3428450.055.44079644
CGTATGC3473850.055.34974341
GCCGTCT3412200.055.30386446
GTATGCC3465350.055.1112542
TCGTATG3497850.054.62598840
CCGTCTT3489700.053.880347
CTGCTTG3521700.053.2137354
AAAGGGG3213600.052.73388368
CGTCTTC3590150.052.46371548
CTCGTAT3507500.052.42009439
ATCTCGT3305950.051.99257337
TCTCGTA3381400.051.61321338
TGCTTGA3657100.051.3527855
CTTCTGC3682250.051.1256951
GCTTGAA3679900.050.9848556
TATCTCG3391800.050.3259736
TCTGCTT3758000.049.8630153
Produced by FastQC (version 0.11.2)