FastQCFastQC Report
Thu 3 Sep 2020
H2NN2BGXF_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NN2BGXF_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381324256
Sequences flagged as poor quality0
Sequence length91
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG4606250.12079614468584973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4470320.11723146192934551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT11527350.073.879891
AGCAGTG11779050.072.139242
GCAGTGG14070200.060.3652233
CAGTGGT15612050.054.408464
AGTGGTA17666250.048.2348485
GTGGTAT20627250.041.3750766
GTATCAA21458850.039.7890789
TGGTATC25699450.033.5565077
GGTATCA27160000.031.803178
TACATGG17737250.026.99647722-23
CAACGCA17814100.026.99056812-13
CATGGGG7370900.026.892224-25
AGAGTAC18089600.026.5951318-19
ACGCAGA18355000.026.22175814-15
AGTACAT18420200.026.09079620-21
GCAGAGT18472950.026.01668416-17
ATCAACG18857550.025.56788310-11
CGCAGAG17490850.024.34998116-17
CATGGGC2647950.024.26456524-25
CAGAGTA17833550.023.90483318-19
Produced by FastQC (version 0.11.5)