FastQCFastQC Report
Thu 3 Sep 2020
H2NN2BGXF_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NN2BGXF_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381324256
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGACG803050.017.5227181
GCCCGAA655500.016.7915041
ATGGGAG663700.016.3502561
GGAGGAT1035950.015.9043681
AATGGCT806950.015.7129121
GATGACT974300.015.6987931
TACGTCC1116000.015.638211
GTACTCG930100.015.6261388
GAAGCCC1069750.015.4851781
GGAATGG708400.015.3900151
AACGGGA753550.015.3645691
AAACGAA967200.015.3077221
GGCAGCG517300.015.2898936
TGACTAG771500.015.0998823
CACTGCG573450.014.8542148
CCCTGAT899450.014.8212891
CCGATGG626400.014.7835171
CGATGGC704950.014.7332011
GCCAACG1143900.014.7238251
GGAACCC932600.014.6555321
Produced by FastQC (version 0.11.5)