FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20521549
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG880251842.894023253312895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGAG604.998346E-429.155539
ACCGAGC755.607165E-527.989319
CGCCGTA2700.024.62262547
CTCGGTG2900.022.91993338
CCGTATC3050.021.79622849
TCGCCGT3250.021.53176146
CCCTACA2302.8740033E-1019.77293610
GTTCGGC2302.8740033E-1019.77288814
CGGTTCG2302.8740033E-1019.77259812
GCCGTAT3450.019.26964648
TCTCGGT3500.018.99380937
CCTACAC2508.749339E-1018.19101111
TAGGTAG2155.6019417E-817.9080415
CGTATCA3850.017.26773350
GATCTCG3900.017.05528635
TCCCTAC2903.45608E-1016.89019
ACTATGC3301.2551027E-1015.9048778
GTATACG3151.1041266E-915.5628531
CTAGGGT1350.0029473215.5567834
TGAACCG3401.9463187E-1015.45403829
Produced by FastQC (version 0.11.5)