FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_bc_anc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_bc_anc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48405
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG510.10536101642392315No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCTT150.002212015270.022
AGCGTTT200.00691207252.516
GCTCTAA200.00691207252.51
CTCTATA200.00691207252.52
TGACTGT200.00691207252.56
TATACCG305.823455E-446.6666645
GGTATTA350.001243890740.01
CTCTAAG350.001243890740.02
TATATAG450.004268198731.1111135
TATACAG450.004268198731.1111135
TCTATAC450.004268198731.1111133
Produced by FastQC (version 0.11.5)