FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_bc_anc4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_bc_anc4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59382
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG980.16503317503620626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTC252.3726856E-455.9993258
GTATTAG407.321469E-752.5436331
CTGCTCT200.006920089552.4993679
GCCATTG200.006920089552.49936754
GTGGCAT200.006920089552.49936729
GGTATCG200.006920089552.49936728
AACTCAC200.006920089552.49936734
TATAAGG305.83455E-446.6661072
AATCAGT450.00427618731.11073956
GCTCTTC450.00427618731.11073911
TATGCTG500.00715668527.9996625
ATAAGGA657.8708224E-426.9227523
TATACAA1001.3700581E-524.4997042
Produced by FastQC (version 0.11.5)