FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_bc_anc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_bc_anc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77367
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG850.10986596352450011No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGA150.00221704670.067
CTGTACC350.00124852339.9999964
TCCTGGA459.122543E-538.888892
CAGTTGA450.004283900431.11111321
TAGAACT450.004283900431.11111355
GGAATTT500.007169515428.00000213
CATTACA800.00264493921.87500243
ATAGAAC900.00523337119.4444453
ATCTAAA950.007151474718.4210511
GTATATA1000.0096113117.5000021
GCTGCCG1400.003711933714.99999970
TGTCTCT7155.9480866E-46.363636570
CTGTCTC7459.775524E-46.107382369
Produced by FastQC (version 0.11.5)