FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_bc_anc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_bc_anc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68171
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG870.12762024907952063No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTCT200.006924148652.59
TGGTTCC200.006924148652.565
ACCGTAG200.006924148652.519
TGGGAGC200.006924148652.538
GACTGGT200.006924148652.537
CCCATCA200.006924148652.539
GTAACAC200.006924148652.53
ACATTAT400.002403552935.06
TGTATAC400.002403552935.03
AACGCTT400.002403552935.065
AATGTGA400.002403552935.063
CGCAAAA450.00428035431.11111364
TCTACCG500.00716361627.9999985
TATTACA500.00716361627.9999982
TATACAC4905.154722E-47.85714370
TTATACA5158.777487E-47.475728569
CTTATAC5400.00145295297.129629668
TCTTATA5750.00281929676.69565267
CTCTTAT5900.00369378926.52542466
TCTCTTA6300.00731723246.111110765
Produced by FastQC (version 0.11.5)