FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_Gbc_c84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_Gbc_c84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43875
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG660.15042735042735042No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCTC150.002210621570.000013
CGGGATA200.0069077552.50000412
CGTAATA200.0069077552.50000413
CACCCAA200.0069077552.50000425
CTCTCCT350.001242608440.00000447
ACGCTGC1200.001313398517.50000270
GACGCTG1200.001313398517.50000269
CTGACGC1400.00367779915.00000167
TGACGCT1450.00464420514.48275968
Produced by FastQC (version 0.11.5)