FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_Gbc_c82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_Gbc_c82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7377
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150.20333468889792597No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGA450.00403842931.11110970
GCCGACG500.006761127.99999869
TGCCGAC901.687558E-423.33333270
GCTGCCG952.4348169E-422.10526568
CGCTGCC1054.7907987E-420.00000267
CTGCCGA1054.7907987E-420.00000269
ACGCTGC1106.556524E-419.09090866
GACGCTG1158.844066E-418.26086865
TGACGCT1200.001177277117.50000264
CTGACGC1350.002591067715.55555663
TCTGACG1350.002591067715.55555662
ATCTGAC1500.005227803314.061
CATCTGA1600.0080207513.12560
Produced by FastQC (version 0.11.5)