FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n02_Gbc_c81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n02_Gbc_c81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78469
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1180.1503778562234768No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTC150.002218766169.987251
ATCAGTT350.001249749939.99271848
GCCCAAG400.00240791534.993631
TTATACC500.007176506327.99494
GTTATAT500.007176506327.99491
GTTGTTG950.00715988118.4176961
CGACGAA2300.007752677410.65702770
GCTGTCT3551.8366975E-49.863646567
TGACGCT4950.004958337.073928470
CTGACGC5000.00546707037.00318969
ATCTGAC6300.0073163436.11389570
CATCTGA7000.00326132666.002733769
GTCTCTT13504.3018917E-75.70630270
ACACATC8050.00245890585.65474968
ACATCTG7500.00702049075.60255168
CACATCT7650.00873400555.492697267
CTGTCTC14351.3948993E-65.36829868
TGTCTCT13703.4290715E-65.367407369
Produced by FastQC (version 0.11.5)